The actin genes of drosophila: a dispersed multigene family

Fyrberg, Eric; Kindle, Karen L.; Davidson, Norman; Sodja, Ann

doi:10.1016/0092-8674(80)90511-5

Cited by 468 publications

(213 citation statements)

References 46 publications

Supporting

Mentioning

205

Contrasting

Unclassified

Order By: Relevance

“…It had been constructed by the ligation of partial AluI HaeIII fragments of Strongylocentrotus purpuratus DNA into the bacteriophage Charon 4 vector by the method of Maniatis et al (9). A hybrid plasmid subclone containing most of the Drosophila meZanogaster actin gene from the XDmA2 recombinant phage (4) was obtained from Dr. N.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus

Schuler

Keller

1981

Nucl Acids Res

View full text Add to dashboard Cite

Four distinct actin genes of the sea urchin Strongylocentrotus purpuratus have been isolated from a recombinant Charon 4 phage library of genomic DNA. The four genes differ considerably from each other in many of their restriction sites. Two of the four genes are closely linked; they are present in the same fragment of cloned DNA. This fragment has been extensively mapped, and some parts of the DNA have been sequenced. The two linked genes are oriented in the same direction, separated by 7.5 kb of DNA. One has an intron following the CAG that codes for the glutamine residue at position 121 in the amino acid sequence of actin. This represents the fifth distinct site at which introns have been found in actin genes, suggesting that the primordial actin gene had at least 6 exons and 5 introns. The actin genes form a distinctive family in which most introns have apparently been precisely excised from the genes.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Actin genes have been cloned from Dictyosteliwn discoidewn, Drosophila, yeast, and rats, and in some cases the DNA has been sequenced (2)(3)(4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus

Schuler

Keller

1981

Nucl Acids Res

View full text Add to dashboard Cite

show abstract

“…Sequencing of the corresponding cDNAs indicated that one encoded a carboxypeptidase (Edwards et al, 1997) and that the other two encoded a cytoplasmic actin, named act1D (Salazar et al, 1994; GenBank accession number XM_001230771). The identity of this cDNA was established by sequencing, its expression pattern and the organization of the gene, all of which were most similar to the characterized cytoskeletal actin, act5C (GenBank accession number P84185) in Drosophila (Fyrberg et al, 1980;Sodja et al, 1982;Fyrberg et al, 1983;Salazar et al, 1994).…”

Section: Resultsmentioning

confidence: 99%

“…They are highly conserved in evolution at both the nucleic and amino acid sequence levels (Fyrberg et al, 1980;Zafar and Sodja, 1983;Mounier et al, 1987;Hadden and Sodja, 1994). In contrast to the high conservation of their amino acid sequence, actins have a broad spectrum of functions.…”

Section: Discussionmentioning

confidence: 99%

“…gambiae actin, characterized in detail by Salazar et al, (1994). Sequencing of this gene, named act1D (GenBank accession number XM_001230771) indicated that it has 99.2% sequence identity with the cytoplasmic/cytoskeletal Drosophila actin, act5C (GenBank accession number P84185) in addition to its gene structure and expression profile (Fyrberg et al, 1980;Sodja et al, 1982;Fyrberg et al, 1983;Salazar et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape

Sodja

Fujioka

Lemos

et al. 2007

Journal of Insect Physiology

Self Cite

View full text Add to dashboard Cite

Ingestion of a blood meal by the female mosquito Anopheles gambiae (L., Diptera: Culicidae), results in a dramatic distention of the midgut epithelium. Here we report that these events correlate with a transient increase of actin mRNA and protein abundance. The newly synthesized actin may provide a pool of actin protein needed to remodel epithelial cell cytoarchitecture. We also document changes in midgut epithelial cell morphology. Upon blood ingestion, the columnar cells flatten accompanied by the loss of microvilli on the lumenal side and the unfolding of the labyrinth on the basal side. These changes correlate with the large increase of epithelial surface area needed to accommodate the blood meal. Actin gene expression, actin synthesis and cell morphology all return to the prefeeding state by 24 h after blood intake.

show abstract

Actin in the Drosophila embryo: Is there a relationship to developmental cue localization?

Bearer¹

1991

BioEssays

View full text Add to dashboard Cite

SummaryRecent genetic manipulations have revealed that the cytoplasm of the early Drosophila embryo contains localized information that specifies the future embryonic axes. It is the restricted distribution or activity of particular gene products, either messenger RNA or protein, that is crucial for this specification. While some of the genes responsible for this information have been sequenced and the nature and distribution of their products examined, it is not known how this localization is established or maintained. The actin-based cytoskeleton is a likely candidate for the formation, of a cytomatrix that would allow such distributions and yet no direct evidence has yet been found that implicates actin in positional cue localization. In this review I summarize what is known about actin filament behavior in Drosophila embryos and compare it to the distribution of positional cues. My purpose is to juxtapose these two bodies of information such that the relationship between them may be revealed.

show abstract

The actin genes of drosophila: a dispersed multigene family

Cited by 468 publications

References 46 publications

The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus

The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus

Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape

Actin in the Drosophila embryo: Is there a relationship to developmental cue localization?

Contact Info

Product

Resources

About