The actinomycetes: their nature, occurrence, activities, and importance

Waksman, Selman A.

doi:10.5962/bhl.title.7278

Cited by 84 publications

(49 citation statements)

References 74 publications

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“…The isolation medium contained (per l distilled water) ; yeast extract (Difco), 0n1 g ; glucose, 0n1 g; K # HPO % , 0n5 g; Na # HPO % , 0n7 g ; KNO $ , 0n1 g ; NaCl, 0n3 g ; MgSO Morphology and pigmentation. For examining the degree of growth, pigmentation and aerial mycelia, isolate LM 042 T was grown on ISP medium 2, oatmeal agar (ISP medium 3) and inorganic salts\starch agar (ISP medium 4) as described by Shirling & Gottlieb (1966), and on glucose\asparagine agar (Waksman, 1950), yeast extract\glucose agar (Asano & Kawamoto, 1986), Bennett agar, Hickey-Tresner agar (Waksman, 1961), yeast extract agar, nutrient agar and tapwater agar. Production of melanin pigment was examined using tyrosine agar (ISP medium 7) and peptone\yeast extract\iron agar (ISP medium 6).…”

Section: Methodsmentioning

confidence: 99%

Catellatospora koreensis sp. nov., a novel actinomycete isolated from a gold-mine cave.

Lee¹,

Kang²,

Hah³

2000

International Journal of Systematic and Evolutionary Microbiology

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A new actinomycete strain, LM 042 T , which was isolated from a gold-mine cave in Kongju, Republic of Korea, is described by phenotypic and genotypic characters. The organism formed short chains of non-motile spores and globose bodies from substrate mycelium. An aerial mycelium was absent. This organism was chemotaxonomically characterized by the presence of mesodiaminopimelic acid, rhamnose, xylose, glucose, mannose and ribose in wholecell hydrolysates (a type II cell wall and a variant of sugar pattern D), a glycolyl type of muramic acid, DNA GMC content of 70 4 mol %, a type PII phospholipid pattern (phosphatidylethanolamine as a diagnostic nitrogenous phospholipid), a tetrahydrogenated menaquinone with 10 isoprene units as a major menaquinone, and fatty acid profiles predominated by iso-branched hexadecanoic acid, iso-branched pentadecanoic acid and heptadcenoic acid. A comparative analysis of 16S rDNA sequences indicated that this organism formed a distinct clade within the evolutionary radiation of the family Micromonosporac eae and clustered with members of the genus Catellatospora. The 16S rDNA similarity values between the isolate and its phylogenetic neighbours, the two subspecies of Catellatospora citrea and Catellatospora tsunoense, were 95 0-95 2 % and 94 9 %, respectively. An equidistant relationship was observed among the isolate, Catellatospora ferruginea and all other members of the Micromonosporac eae genera (levels of similarity 93 0-94 0 %). The combination of physiological, chemotaxonomic and DNA-DNA hybridization data supported that this organism is a novel species of the genus Catellatospora, for which the name Catellatospora koreensis sp. nov. is proposed. The type strain is LM 042 T (l IMSNU 50729 T ).

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Section: Methodsmentioning

confidence: 99%

Catellatospora koreensis sp. nov., a novel actinomycete isolated from a gold-mine cave.

Lee¹,

Kang²,

Hah³

2000

International Journal of Systematic and Evolutionary Microbiology

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“…Streptomycetes are widely distributed in soils, especially in those which are dry and not too acid, and those rich in organic matter; here, in propagule numbers, they frequently exceed the combined counts of all other bacteria (Alexander, 1961 ;Waksman, 1959). They decompose plant and animal residues, for the addition of these residues to soil greatly increases the activity and size of the streptomycete population (Alexander, 1961 ;Waksman, 1959).…”

Section: Introductionmentioning

confidence: 99%

Behaviour of Streptomycetes in Soil

Lloyd

1969

Journal of General Microbiology

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“…For cultural characterization, the isolates were grown for 21 days at 30 u C on various agar media as described by Waksman (1950Waksman ( , 1961, Shirling & Gottlieb (1966) and Asano & Kawamoto (1986). Names and designations of colony colour were based on Jacobson et al (1958).…”

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confidence: 99%

Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov.

Ara

Matsumoto

Bakir

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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The taxonomic position of two soil isolates, 3-46-b(3) T and 3-45-a(11) T , was clarified based on data from a polyphasic study. The organisms showed a combination of chemotaxonomic and morphological properties typical of the genus Actinomadura. They formed distinct phyletic lines in the Actinomadura 16S rRNA gene tree and were closely associated with the type strains of Actinomadura meyerae (sequence similarity of 98.3-98.5 %), Actinomadura napierensis (98.1-98.3 %) and Actinomadura latina (96.4-96.8 %). The level of 16S rRNA gene sequence similarity between the new isolates was 99.1 %. The level of DNA-DNA hybridization between strains 3-46-b(3) T and 3-45-a(11) T was 43.6 % and levels of relatedness between the two new isolates and the type strains of A. meyerae and A. napierensis were 21.0-27.3 %. On the basis of phenotypic and genotypic properties, the new isolates could be differentiated from each other and from their closest phylogenetic relatives. It is proposed that the organisms be classified as representing two novel species of the genus Actinomadura. The names proposed for these taxa are Actinomadura bangladeshensis sp. nov. [type strain 3-46-b(3) T 5JCM 13933 T 5MTCC8057 T ] and Actinomadura chokoriensis sp. nov. [type strain 3-45-a(11) T 5JCM 13932 T 5MTCC8056 T ].The application of chemosystematic, numerical phenetic and molecular systematic methods has clarified the taxonomy of the genus Actinomadura and related taxa (Poscher et al., 1985;Kroppenstedt et al., 1990;Ochi et al., 1991;Trujillo & Goodfellow, 1997Quintana et al., 2003a;Lee & Jeong, 2006;Le Roes & Meyers, 2007 (Henssen, 1957;Zhang et al., 2001). Members of these genera form distinct phyletic lines in trees based on 16S rRNA gene sequences (Zhang et al., 1998(Zhang et al., , 2001) and can be distinguished from one another by using a combination of biochemical, chemical, morphological and physiological features (Zhang et al., 1998(Zhang et al., , 2001Trujillo & Goodfellow, 2003). At the time of writing, the genus Actinomadura encompasses 37 recognized species, which can be delineated based on a combination of genotypic and phenotypic data (Kroppenstedt & Goodfellow, 1991;Zhang et al., 2001;Lu et al., 2003;Lee & Jeong, 2006;Le Roes & Meyers, 2007).As part of a screening programme for antibiotic-producing actinomycetes, strains 3-46-b(3) T and 3-45-a(11) T were isolated from sandy soil collected at Chokoria, Cox's Bazar, Bangladesh. The two strains showed morphological characteristics typical of the genera Actinomadura and Nonomuraea. Here we describe the characterization and classification of these strains and propose that they represent two novel species of the genus Actinomadura.Bacterial strains were isolated by using the standard dilution plate method and were grown on humic acid-vitamin agar (Hayakawa & Nonomura, 1987) supplemented with cycloheximide (50 mg l 21 ), nystatin (50 mg l 21 ) and A neighbour-joining tree based on nearly-complete 16S rRNA gene sequences showing the relationships between strains 3-46-b(3) T , 3-45-a(11) T a...

show abstract

The actinomycetes: their nature, occurrence, activities, and importance

Cited by 84 publications

References 74 publications

Catellatospora koreensis sp. nov., a novel actinomycete isolated from a gold-mine cave.

Catellatospora koreensis sp. nov., a novel actinomycete isolated from a gold-mine cave.

Behaviour of Streptomycetes in Soil

Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov.

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