The action and mechanism of myrislignan on A549 cells in vitro and in vivo

Abstract

Myrislignan is a natural compound with little pharmacological study. In our investigation, we investigated the effect of myrislignan in the induction of apoptosis in A549 cells in vitro and in vivo. Myrislignan inhibited the proliferation of A549 cells in a dose- and time-dependent manner assayed by MTT. In addition, Hoechst flow cytometry showed that myrislignan significantly induced apoptosis and cell cycle arrest in A549 cells. The apoptosis and anti-cell proliferation was mediated by the activation of mitogen-activated protein kinase and the inhibition of epidermal growth factor receptor signal pathway, change of mitochondrial membrane potential, the releasing of c-Myc, the downregulation of the level of the anti-apoptotic protein Bcl-2, and the upregulation of the level of the pro-apoptotic protein Bax. In conclusion, those results reveal a potential mechanism for the anti-cancer effect of myrislignan on human lung cancer, while suggesting that myrislignan may be a promising compound for the treatment of lung cancer.

Chemical Compounds and Biologic Activities: A Review of Cedrela Genus

“…With the purpose of evaluating the anti-cancer capacity of compound 127, Wan et al [49] verified through western blot assay based on Lu et al [88] the regulatory roles of compound 127 in ROS/mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling pathways and effects of these on the autophagy, migration, and invasion. N-acetyl-l-cysteine (NAC) was used as a ROS remover was able to suppress ROS production, and the role of compound 127 in MEK/ERK signaling.…”

GC-MS Analysis of the Composition of the Extracts and Essential Oil from Myristica fragrans Seeds Using Magnesium Aluminometasilicate as Excipient

“…Elemicin has antibacterial effects [29] and α-phellandrene, anti-inflammatory [30], so using maceration with water is more effective if a bigger effect is needed. Myrislignan has anti-inflammatory [31] and anti-cancer [32] effects. This compound was not extracted using ethanol [8].…”

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“…[78] A study of myrislignan on A549 cells in vitro and in vivo reveal a potential mechanism for the anticancer effect of myrislignan on human lung cancer, while suggesting that myrislignan may be a capable compound for the management of lung cancer. [79] …”

“…Antibacterial activity [62][63][64][65][66][67] Trimyristin and myristic acid, [66] macelignan [67] Insecticidal and repellent activity [64,68] Essential oil [64] Antiamebic activity [69,70] Essential oil [70] Antifungal activity [64,72] Lignans-EA6, MDA and NB, [71] sabinene, terpinen-4-ol, safrole, α-pinene, β-phellandrene, and γ-terpinene [64] Hypolipidemic activity [73] Ethanolic extract [73] Antioxidant activity [60,74,75] Eugenol [74] Anticancer activity [76][77][78][79] Myrislignan [79] Aphrodisiac activity [80,81] Ethanolic extract [80,81] Anxiogenic, analgesic and sedative activities [82,83] N-hexane extract and trimyristin [82] Antidepressant activity [84] N-hexane extract [84] Anti-inflammatory activity [85,86] 8-O-4' type neolignans, named myrifralignan A-E (1-5) [85] Hepatoprotective activity [87,88] Myristicin, [87] macelignan [88]...…”