The action of antidiuretic hormone on cell membranes. Voltage transient studies

Cuthbert, A. W.; Painter, Elisabeth

doi:10.1111/j.1476-5381.1969.tb07965.x

Cited by 14 publications

(7 citation statements)

References 25 publications

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“…In the second (Fig. 7b) (Cuthbert & Painter, 1969). This factor together with the slowly developing response to ADH and the low and unsteady skin resistance make it difficult to demonstrate convincingly a change in resistance with ADH in the current clamped skin.…”

Section: Resultsmentioning

confidence: 99%

“…pumping by an action on phosphorylase, so that the intracellular Na+ concentration became low enough to account for the increased skin resistance. It is possible to divide the skin resistance into the cell membrane resistance (Rp) and the cytoplasmic resistance (Rs) (Cuthbert & Painter, 1969). In Fig.…”

Section: Adh Action On Frog Skinmentioning

confidence: 99%

“…It is possible to divide the skin resistance into the cell membrane resistance (Rp) and the cytoplasmic resistance (Rs) (Cuthbert & Painter, 1969). In Fig.…”

Section: Adh Action On Frog Skinmentioning

confidence: 99%

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Independent action of antidiuretic hormone, theophylline and cyclic 3′,5′‐adenosine monophosphate on cell membrane permeability in frog skin

Cuthbert¹,

Painter²

1968

The Journal of Physiology

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1. The effects of antidiuretic hormone (ADH), theophylline and cyclic 3′,5′‐adenosine monophosphate (AMP) on membrane potentials in frog skin have been investigated. 2. Membrane potentials across the outer and inner facing membranes were recorded in both normal and current clamped skins. In the latter condition active transport of sodium had been abolished by ouabain or metabolic inhibitors, but ionic gradients were maintained by passing current through the skin from the inside. 3. ADH increases the potential across the outer facing membranes and reduces the skin resistance. The results are consistent with ADH causing an increase in permeability of the outer facing membranes to sodium ions. 4. Theophylline reduces the skin potential by reducing specifically the potential across the outer facing membranes. At the same time the skin resistance is reduced. Theophylline acts by increasing the permeability of the outer facing membranes to chloride ions. 5. Cyclic 3′,5′‐AMP causes a biphasic potential change accompanied by an increase in skin resistance. 6. Metabolic inhibitors block the response of the skin to ADH but not to theophylline. 7. Separate explanations for the increase in sodium transport by ADH, theophylline and cyclic 3′,5′‐AMP are discussed. It is not necessary to involve cyclic AMP in order to explain the effects of either ADH or theophylline.

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Section: Resultsmentioning

confidence: 99%

Section: Adh Action On Frog Skinmentioning

confidence: 99%

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Independent action of antidiuretic hormone, theophylline and cyclic 3′,5′‐adenosine monophosphate on cell membrane permeability in frog skin

Cuthbert¹,

Painter²

1968

The Journal of Physiology

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“…Teorell [24] and Cuthbert and Painter [5,6] determined the parameters of the network from an approximate reconstruction of the frequency response by Fourier analysis [1], and a similar procedure was used here. Calculations were carried out with the aid of the University of Liverpool's (5) and (6) became zero.…”

Section: Analysis Ofmentioning

confidence: 99%

“…In these experiments, therefore, the capacitance was determined from the response of the p.d. across the skin to the passage of a step of current [5,6,24] which can be measured much more quickly.…”

Section: Measurement Of Capacitancementioning

confidence: 99%

Aldosterone-induced moulting in amphibian skin and its effect on electrical capacitance

Smith

1975

J. Membrain Biol.

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The resistance and capacitance of the isolated amphibian skin have been determined from measurements of the response of the voltage across the skin to small steps to current. Previous work indicates that the electrical impedance of frog skin, when the skin is bathed with Ringer's solution on both sides, is largely determined by the properties of the functional outward-facing membrane of the skin, the outer membrane of the stratum granulosum (P.G. Smith, 1971, Acta Physiol. Scand. 81:355). This membrane can be represented by a resistance and capacitance in parallel. Aldosterone, which induces conversion of the s. granulosum into a cornified cell layer and transformation of the cell layer below into a new s. granulosum, also causes a transient rise in resistance and a short-lived decrease in capacitance to about one-half its initial value. It is suggested that these electrical changes are caused by the transitory presence of two functional outward-facing membranes in series. The method of determining resistance and capacitance from the voltage response is discussed in the Appendix.

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Rapid determination of intraepithelial resistance barriers by alternating current spectroscopy

Kottra

Frömter

1984

Pflugers Arch.

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The impedance of Necturus gallbladder epithelium was measured with transepithelial and intracellular microelectrodes in different transport states. The data are analysed with five electrical equivalent circuits, which differ with respect to the configuration of the paracellular shunt path (lumped vs distributed model of the lateral space), and of the apical cell membrane (non-ideal capacitance or surface amplification by micro-tubular infoldings). Least square fits indicate: 1. that the lumped model cannot represent the epithelium properly, even under control conditions; 2. that the distributed model, which considers the lateral intracellular space separately as a cable-like structure, describes the data well, both under control conditions and during collapse of the lateral spaces; and 3. that the above indicated variations of the apical membrane configuration improve the fits, but have little effect on the magnitude of the calculated circuit parameters. Quantitatively the analysis of 214 measurements on 25 gallbladders under control conditions yields the following results: The resistances of the tight junctions, of the lateral intercellular space, and of the apical and basal cell membrane are Rj = 123, Rlis = 35.5, Ra approximately 3,500, and Rb = 225 (all in omega cm2), and the capacitances of the cell membranes are Ca = 4.95 and Cbl = 26.5 (mu F/cm2). In oxygen deficiency and after cessation of chamber perfusion transport decreased, the spaces collapsed, and Rlis increased to approximately 130 omega cm2. Although the accuracy of the estimated Ra values is still limited, the analysis shows that it is possible to determine Rj and Rlis separately and to follow their changes in response to experimental maneuvers.

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The action of antidiuretic hormone on cell membranes. Voltage transient studies

Cited by 14 publications

References 25 publications

Independent action of antidiuretic hormone, theophylline and cyclic 3′,5′‐adenosine monophosphate on cell membrane permeability in frog skin

Independent action of antidiuretic hormone, theophylline and cyclic 3′,5′‐adenosine monophosphate on cell membrane permeability in frog skin

Aldosterone-induced moulting in amphibian skin and its effect on electrical capacitance

Rapid determination of intraepithelial resistance barriers by alternating current spectroscopy

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