The Action of Atrazine Herbicide as an Inhibitor of Cell Division on Chromosomes and Nucleic Acids Content in Root Meristems of Allium cepa and Vicia faba.

El-Ghamery, Abbas A.; El-Nahas, A. I.; Mansour, Mahmoud

doi:10.1508/cytologia.65.277

Cited by 121 publications

(67 citation statements)

References 19 publications

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“…Anaphase bridges could happen during the translocation of the unequal chromatid exchange, due to dicentric chromosome presence, or due to the breakage and fusion of chromosomes and chromatids. (El-Ghamery et al 2000;Luo et al 2004). In addition, bridges may arise due to chromosome breaks, stickiness and breakage and reunion of the broken ends (Badr et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC 50 value was determined 50 ppm and 1/29 EC 50 (25 ppm), EC 50 (50 ppm) and 2 9 EC 50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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Section: Discussionmentioning

confidence: 99%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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“…Bridges were found to be the result of stickiness of chromosome (El-Ghamery et al 2000). Chromosomal bridges mainly arises due to the non disjunction of sticky chromosome or breakage and reunion during separation at anaphase (Koduru and Rao 1981).…”

Section: Resultsmentioning

confidence: 99%

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

et al. 2016

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2,4-Dichlorophenoxyacetic acid (2,4-D) is a synthetic plant growth regulator that is highly toxic to most broad leaved plants and relatively nontoxic to monocotyledonous plants; is frequently used as weed killer. The study aimed to investigate cytogenetic effects of different concentrations of 2,4-D (0.67, 1.34, 2.01, 2.68, 3.35 and 4.02 mg/L) on Allium cepa bulblets' root tips treated for 24 and 48 h. The results showed six types of structural aberrations: C-mitosis, stickiness, laggards, bridges, fragments and multipolarity that varied numerically compared to control. It significantly affected mitotic index (MI) at 24 and 48 h treatment. In the Allium test, MI increased significantly at three lower concentrations (0.67, 1.34, 2.01 mg/L) after treatment with 2,4-D for 24 h and decreased significantly at higher concentration. Whereas, 2,4-D treatment for 48 h increased MI at all concentrations with significantly decreased MI at the highest concentration. The experiment was extended using comet test that did not reveal significant difference among treatments except for application of 4.02 mg/L 2,4-D for 48 h; where cell damages were verified by comet test. Rest of the concentrations for any duration of time were not damaging and toxic to cells. The results showed, visible mitodepressive action of 4.02 mg/L 2,4-D when treated for 48 h that had tendency to become toxic if the roots had been in touch with 2,4-D for a longer time.

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“…Anaphase bridges could happen during the translocation of the unequal chromatid exchange or due to dicentric chromosome presence or due to the breakage and fusion of chromosomes and chromatids. This bridges cause structural chromosome mutations (El-Ghamery et al, 2000;Luo et al, 2004). Disturbed anaphase-telophase (especially at 15 g/L in 12 h and at 7.5 g/L in 72 h) and chromosome laggards could occur by the effect of Lg-ext on microtubule formations (Amer & Ali, 1986;Kumari et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Liman¹,

Gökçe²,

Akyıl³

et al. 2012

Rev. bras. farmacogn.

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Genotoxic and mutagenic effects of aqueous extract from aerial parts Linaria genistifolia (L.) Mill. subsp. genistifolia, Plantaginaceae (Lg-ext) were investigated by using both Allium cepa root meristematic cells and bacterial reverse mutation assay in Salmonella typhimurium TA98 and TA100 with or without metabolic activation system (S9), respectively. In Allium root growth inhibition test, EC50 value was determined approximately 15 g/L and 0.5xEC50, EC50 and 2xEC50 concentrations of Lg-ext were introduced to onion tuber roots and distilled water and methyl methane sulfonate (MMS, 10 ppm) used as a negative and positive control, respectively. The characteristic effect caused by tested preparations was an increase of mitotic index (MI) in 7.5 g/L and 15 g/L (except 24 and 96 h) and simultaneous decrease of MI in 30 g/L and in MMS. While stickiness, bridges, chromosome laggards and disturbed anaphase-telophase were observed in anaphase-telophase cells, c-metaphase, pro-metaphase, polyploidy and binuclear cells were observed in other cells. Lg-ext was not found to be mutagenic on S. typhimurium TA 98 and TA100 with or without S9. The results were also analyzed statistically by using SPSS for Windows, and Duncan's multiple range tests were performed respectively. These results indicate that Lg-ext exhibits genotoxic activity in A. cepa root meristematic cells but not mutagenic activity in Ames test system.

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The Action of Atrazine Herbicide as an Inhibitor of Cell Division on Chromosomes and Nucleic Acids Content in Root Meristems of Allium cepa and Vicia faba.

Cited by 121 publications

References 19 publications

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

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