The Action of Efflux Pump Genes in Conferring Drug Resistance to Klebsiella Species and Their Inhibition

Ashwath, Priyanka; Akhila, Dharnappa Sannejal

doi:10.1055/s-0041-1731914

Cited by 5 publications

(2 citation statements)

References 81 publications

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“…Gramnegative bacteria evade drugs via multidrug efflux pumps. Efflux systems make microorganisms more resistant (6) . K. pneumoniae use resistance-nodulationdivision (RND) efflux pumps OqxAB and AcrAB to become antibiotic-resistant (7) .…”

Section: Introductionmentioning

confidence: 99%

Effect the Natural Efflux Pump Inhibitor (Berberine) in Multidrug Resistant Kleibsiella pneumoniae Isolated from Urinary Tract Infections in Several Baghdad Hospitals

Khalid¹,

Ghaima²

2022

The Egyptian Journal of Hospital Medicine

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Background: Kleibsiella pneumoniae in urinary tract infections (UTIs) have grown for years. Antibiotic-resistant microorganisms make infection control and treatment difficult for clinicians. Efflux pumps contribute to K. pneumoniae's multidrug resistance.Objective: This study aimed to test Phenylalanine-Arginine β-Naphthylamide (PAβN) and Berberine as antibacterial agents against multidrug-resistant bacteria (UTI K. pneumonia isolates). Subjects and Methods: Between December 2021 and April 2022, five Baghdad hospitals collected 260 urine samples from outpatients and inpatients with urinary tract infections, both genders, aged 15 to 72. Results: We isolated 76 of the cultures (65.5%) and employed selective media, biochemical assays, and the VITEK2 system to identify bacteria as K. pneumoniae. Using the disc diffusion technique, it was demonstrated that clinical isolates of K. pneumoniae were moderately resistant to the majority of drugs. K. pneumoniae isolates were extremely resistant to Amoxicillin (96.1%), Trimethoprim (80.3%), Gentamicin, Amikacin and Meropenem (55.1%), and Ciprofloxacin (53.9%). The efflux pump inhibitor (PAβN) greatly boosted strain susceptibility to Ciprofloxacin. PAβN lowered MICs by 4-64-fold. The minimum inhibitory concentrations (MICs) of Berberine against ten multidrug-resistant K. pneumoniae isolates ranged from 3.9 to 500 μg/ml, indicating that Berberine affects K. pneumoniae growth at very low doses. Conclusion:In this work, Berberine was found to be an efflux pump inhibitor (EPI) and antibacterial drug that could overcome bacterial resistance mechanisms.

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Section: Introductionmentioning

confidence: 99%

Effect the Natural Efflux Pump Inhibitor (Berberine) in Multidrug Resistant Kleibsiella pneumoniae Isolated from Urinary Tract Infections in Several Baghdad Hospitals

Khalid¹,

Ghaima²

2022

The Egyptian Journal of Hospital Medicine

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“…Three 1 2 3 have some diagnostic care component and the subject of the other three is the laboratory diagnosis of COVID-19. 4 5 6 Since JHAS-NU began in 2016, there have been 2 publications on biofilms, 7 8 3 publications on the microbiome, 9 10 11 2 publications on nanotechnology, 12 13 and 1 publication on probiotics. 14…”

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confidence: 99%

New Research Areas in Clinical Microbiology

Gurtler

2023

Journal of Health and Allied Sciences NU

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I would like to outline a few areas in Clinical Microbiology that have become major research disciplines over the last 10 years that may now impact Health and Allied Sciences. They encompass (1) COVID-19, (2) biofilms, (3) microbiomes, (4) nanotechnology, and (5) probiotics. As well as being direct research areas, they overlap with each other, and they are beginning to overlap in important ways with Health and Allied Sciences-hence the importance of contributing papers on these subjects to the Journal of Health and Allied Sciences NU (JHAS-NU).There have been 43 publications on COVID-19 in JHAS-NU since the journal began in 2016, but the subject of all except for 6 of these articles is direct patient care. Three 1-3 have some diagnostic care component and the subject of the other three is the laboratory diagnosis of COVID-19. [4][5][6] Since JHAS-NU began in 2016, there have been 2 publications on biofilms, 7,8 3 publications on the microbiome, 9-11 2 publications on nanotechnology, 12,13 and 1 publication on probiotics. 14 The following are some examples of current research impacting Health and Allied Sciences in the above-mentioned areas:

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