The action of free radicals on Deinococcus radiodurans carotenoids

Carbonneau, Marie‐Annette; Melin, Anne-Marie; Perromat, Annie; Clerc, M

doi:10.1016/0003-9861(89)90370-6

Cited by 67 publications

(43 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For these authors and Mathews and Sistrom (33), carotenoid pigments appeared to contribute to the resistance to UV irradiation. Carotenoids were found to protect microorganisms from UV and visible light damage by quenching triplet-state photosensitizers and ROS (7,32). In the surface microlayer of the Black Sea, the number of pigmented cells, primarily yellow, often exceeded that in underlying waters (41).…”

Section: Discussionmentioning

confidence: 99%

Resistance of Marine Bacterioneuston to Solar Radiation

Agogué

Joux

Obernosterer

et al. 2005

Appl Environ Microbiol

137

106

View full text Add to dashboard Cite

A total of 90 bacterial strains were isolated from the sea surface microlayer (i.e., bacterioneuston) and underlying waters (i.e., bacterioplankton) from two sites of the northwestern Mediterranean Sea. The strains were identified by sequence analysis, and growth recovery was investigated after exposure to simulated solar radiation. Bacterioneuston and bacterioplankton isolates were subjected to six different exposure times, ranging from 0.5 to 7 h of simulated noontime solar radiation. Following exposure, the growth of each isolate was monitored, and different classes of resistance were determined according to the growth pattern. Large interspecific differences among the 90 marine isolates were observed. Medium and highly resistant strains accounted for 41% and 22% of the isolates, respectively, and only 16% were sensitive strains. Resistance to solar radiation was equally distributed within the bacterioneuston and bacterioplankton. Relative contributions to the highly resistant class were 43% for ␥-proteobacteria and 14% and 8% for ␣-proteobacteria and the Cytophaga/Flavobacterium/Bacteroides (CFB) group, respectively. Within the ␥-proteobacteria, the Pseudoalteromonas and Alteromonas genera appeared to be highly resistant to solar radiation. The majority of the CFB group (76%) had medium resistance. Our study further provides evidence that pigmented bacteria are not more resistant to solar radiation than nonpigmented bacteria.

show abstract

Section: Discussionmentioning

confidence: 99%

Resistance of Marine Bacterioneuston to Solar Radiation

Agogué

Joux

Obernosterer

et al. 2005

Appl Environ Microbiol

137

106

View full text Add to dashboard Cite

show abstract

“…Note that, as even the wild-type C. melassecola ATCC 17965 strain cannot grow in M63, the control experiment was not possible. Carotenoid pigments can play a role in singlet oxygen and also superoxide scavenging, (8,12,49). Carotenoids have been identified in some Corynebacterium species (54,77).…”

Section: Discussionmentioning

confidence: 99%

Cloning of the sodA Gene from Corynebacterium melassecola and Role of Superoxide Dismutase in Cellular Viability

Merkamm

Guyonvarch

2001

J Bacteriol

View full text Add to dashboard Cite

The sodA gene encoding the Corynebacterium melassecola manganese-cofactored superoxide dismutase (SOD) has been cloned in Escherichia coli and sequenced. The gene is transcribed monocistronically; the predicted polypeptide is 200 amino acids long and associates in a homotetrameric, manganese-dependent form, able to complement an SOD-deficient E. coli mutant. A second open reading frame, coding for a putative 217-aminoacid protein with high homology to peptide methionine sulfoxide reductases from various origins, has been identified immediately upstream of sodA in the opposite transcription orientation. The sodA gene was inactivated by insertion of an integrative vector carrying a kanamycin resistance gene. The growth rate of the SOD-deficient integrant was only slightly affected in BHI rich medium as well as in BMCG chemically defined medium, but was strongly affected by the presence of the redox-cycling agent paraquat. The SOD deficiency had, on the other hand, a deleterious effect on viability as soon as the culture entered the stationary phase of growth in BHI medium. Surprisingly, SOD deficiency was able to rescue the dramatic loss of viability observed for the wild-type strain in BMCG synthetic medium when glucose was not the limiting growth factor.Reactive oxygen species are formed in aerobic organisms as by-products of normal metabolism, as a consequence of partial reduction of molecular oxygen to superoxide anion (O 2 ⅐Ϫ ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical ( ⅐ OH). To protect cells from damage by these reactive species to DNA, proteins, and lipids, aerobic organisms have evolved detoxification and repair systems (69). Superoxide dismutase (SOD; EC 1.15.1.1) is considered a key enzyme in oxygen defense systems by catalyzing the dismutation of O 2 ⅐Ϫ into oxygen and H 2 O 2 (22), the latter being broken in turn to water by catalase or peroxidase.SODs are classified in different groups depending on the type of metal cofactors: copper-and zinc-containing (Cu,Zn-SOD) (46), iron-containing (Fe-SOD) (83), manganese-containing (Mn-SOD) (39), and nickel-containing (Ni-SOD) (84) enzymes. Another type of small metalloprotein with superoxide dismutase activity, unrelated to classical SODs, has also been recently identified in the sulfate-reducing bacterium Desulfovibrio (65). Most bacteria possess an Mn-SOD or an Fe-SOD in their cytoplasm, while Cu,Zn-SODs have been identified in the periplasm or periphery of pathogenic and endosymbiotic bacteria (4, 5, 80). The Mn-and Fe-SODs have highly similar sequences and structures, usually have a strict metal selectivity, and are evolutionarily unrelated to other SODs. On the other hand, some SODs have been found to be active with either manganese or iron incorporated into the same apoprotein (47,63,82). These enzymes were named cambialistic. Some bacteria, like Escherichia coli and Pseudomonas aeruginosa, have both an Fe-SOD and an Mn-SOD, which differ not only in their primary sequences but also in their regulation (29,72) and, at least for E. coli, in the...

show abstract

“…this, DRA0050, which is orthologous to the cyanobacterial and plant phytochromes, has been shown to be a photoreceptor involved in the regulation of pigment biosynthesis (55), which is likely to affect resistance to DNA-damaging agents (35). Genes encoding two proteins that consist of a sensory transduction histidine kinase and a receiver domain (DRB0028 and DRB0029) appear to be coregulated with an s B operon (DRB0024 to DRB0027).…”

Section: Dra0145mentioning

confidence: 99%

Genome of the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans Viewed from the Perspective of Comparative Genomics

Makarova

Aravind

Wolf

et al. 2001

Microbiol Mol Biol Rev

656

612

View full text Add to dashboard Cite

The bacterium Deinococcus radiodurans shows remarkable resistance to a range of damage caused by ionizing radiation, desiccation, UV radiation, oxidizing agents, and electrophilic mutagens. D. radiodurans is best known for its extreme resistance to ionizing radiation; not only can it grow continuously in the presence of chronic radiation (6 kilorads/h), but also it can survive acute exposures to gamma radiation exceeding 1,500 kilorads without dying or undergoing induced mutation. These characteristics were the impetus for sequencing the genome of D. radiodurans and the ongoing development of its use for bioremediation of radioactive wastes. Although it is known that these multiple resistance phenotypes stem from efficient DNA repair processes, the mechanisms underlying these extraordinary repair capabilities remain poorly understood. In this work we present an extensive comparative sequence analysis of the Deinococcus genome. Deinococcus is the first representative with a completely sequenced genome from a distinct bacterial lineage of extremophiles, the Thermus-Deinococcus group. Phylogenetic tree analysis, combined with the identification of several synapomorphies between Thermus and Deinococcus, supports the hypothesis that it is an ancient group with no clear affinities to any of the other known bacterial lineages. Distinctive features of the Deinococcus genome as well as features shared with other free-living bacteria were revealed by comparison of its proteome to the collection of clusters of orthologous groups of proteins. Analysis of paralogs in Deinococcus has revealed several unique protein families. In addition, specific expansions of several other families including phosphatases, proteases, acyltransferases, and Nudix family pyrophosphohydrolases were detected. Genes that potentially affect DNA repair and recombination and stress responses were investigated in detail. Some proteins appear to have been horizontally transferred from eukaryotes and are not present in other bacteria. For example, three proteins homologous to plant desiccation resistance proteins were identified, and these are particularly interesting because of the correlation between desiccation and radiation resistance. Compared to other bacteria, the D. radiodurans genome is enriched in repetitive sequences, namely, IS-like transposons and small intergenic repeats. In combination, these observations suggest that several different biological mechanisms contribute to the multiple DNA repair-dependent phenotypes of this organism

show abstract

The action of free radicals on Deinococcus radiodurans carotenoids

Cited by 67 publications

References 22 publications

Resistance of Marine Bacterioneuston to Solar Radiation

Resistance of Marine Bacterioneuston to Solar Radiation

Cloning of the sodA Gene from Corynebacterium melassecola and Role of Superoxide Dismutase in Cellular Viability

Genome of the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans Viewed from the Perspective of Comparative Genomics

Contact Info

Product

Resources

About