The action of phyltoxin on red cell membrane

Chhatwal, G.S.; Hessler, H.-J.; Béress, L.; Habermann, E.

doi:10.1016/0041-0101(83)90155-1

Cited by 1 publication

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mammalian neurotoxins are known to inhibit specifically the Na+ channel, and they have been used to study that Na+ channel (Ovchinnikov & Grishin, 1982). A proteinase inhibitor that specifically inhibits pepsin and kallikrein, and a histamine-releasing factor that is composed of 33 amino acid residues, have been purified (Chhatwal & Habermann, 1982). Separation and partial characterization of smooth-muscle-contractile material in the venom of the scorpion Heterometrus bengalensis has also been reported (Kar et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Purification and N-terminal partial sequence of anti-epilepsy peptide from venom of the scorpion Buthus martensii Karsch

Zhou

Yang

Zhang

et al. 1989

Biochemical Journal

View full text Add to dashboard Cite

An anti-epilepsy peptide (AEP) was isolated and purified from venom of the scorpion Buthus martensii Karsch. The purification procedure included CM-Sephadex C-50 chromatography, gel filtration on Sephadex G-50 and DEAE-Sephadex A-50 chromatography. Its homogeneity was demonstrated by pH 4.3 polyacrylamide-disc-gel electrophoresis, focusing electrophoresis and SDS/polyacrylamide-disc-gel electrophoresis. The Mr of this peptide, calculated from measurements in SDS/15%-polyacrylamide-disc-gel and SDS/20%-polyacrylamide-disc-gel electrophoresis, is 8300. The isoelectric point is 8.52 by pH 8-9.5-range isoelectric focusing. No haemorrhagic or toxic activities were found. No toxicity was found even after the dose reached 28 mg/kg. The pharmacological tests showed that the AEP had no effect on heart rate, blood pressure or electrocardiogram, but strongly inhibited epilepsy induced by coriaria lactone and cephaloridine. The fluorescence spectrum showed that the peptide has a strong emission peak at 337 nm. Amino acid analysis suggested that the AEP is composed of 66 residues from 18 amino acids and has an Mr of 8290. The sequence of the first 50 N-terminal residues is as follows: Asp-Gly-Tyr-Ile-Arg-Gly-Ser-Asp-Asn-Cys-Lys-Val-Ser-Cys-Leu-Leu-Gly-Asn- Glu-Gly - Cys-Asn-Lys-Glu-Cys-Arg-Ala-Tyr-Gly-Ala-Ser-Tyr-Gly-Tyr-Cys-Trp-Thr-Val- Lys-Leu - Ala-Gln-Asp-Cys-Glu-Gly-Leu-Pro-Asp-Thr-.

show abstract

Section: Introductionmentioning

confidence: 99%