THE ACTIONS OF 5‐HYDROXYTRYPTAMINE AND HISTAMINE ON THE ISOLATED ILEUM OF THE TREE SHREW (<i>Tupaia glis</i>)

Sakai, Kazushige; Shiraki, Yasuyuki; Tatsumi, Tetsuo; Tsuji, Koji

doi:10.1111/j.1476-5381.1979.tb10845.x

Cited by 22 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was suggested that both neuronal and non-neuronal 5-HT receptors in the lon gitudinal muscle of guinea pig ileum were involved in the 5-HT-induced contraction (1)(2)(3)(4)(5)(6)(7)(8). Although the nature of the non-neuronal 5-HT receptor remains unclear, the neuronal 5-HT receptor was defined as the 5-HT3 and 5 HT4 receptor subtypes which mediated acetylcholine re lease (7).…”

Section: Resultsmentioning

confidence: 99%

“…In the gastrointestinal tract, 5-HT is reported to contribute to the regulation of the motility and secretion via several 5-HT-receptor subtypes. The in volvement of the 5-HT-receptor subtype in 5-HT-induced intestinal contraction has been well-examined in the guinea pig small and large intestines (1)(2)(3)(4)(5)(6)(7)(8). In contrast, the effect of 5-HT on the regulation of intestinal motility has not been established in species other than guinea pigs.…”

Section: -Hydroxytryptaminementioning

confidence: 99%

See 1 more Smart Citation

Species Differences in the 5-Hydroxytryptamine-Induced Contraction in the Isolated Distal Ileum.

Yamano¹,

Ito²,

Miyata³

1997

Jpn.J.Pharmacol

View full text Add to dashboard Cite

ABSTRACT-We investigated the mechanism of 5-hydroxytryptamine (5-HT)-induced contraction in the longitudinal muscle of isolated distal ileum from ferrets, piglets and rats. 5-HT and 5-methoxytryptamine concentration-dependently contracted the ileum of ferrets, piglets and rats. 2-Methyl-5-HT and m-chloro phenylbiguanide concentration-dependently contracted the ferret ileum, whereas they had no effect in piglets and rats. In ferrets, the 5-HT-induced contraction was inhibited by methysergide and by ramosetron, but not by ketanserin or GR113808. Atropine and tetrodotoxin suppressed contractions elicited by 5-HT, 2-methyl-5-HT and m-chlorophenylbiguanide in ferrets, but not that elicited by 5-methoxytryptamine. In piglets, 5-HT-induced contraction was inhibited by methysergide and by tetrodotoxin, but not by ketan serin, ramosetron, GR113808 or atropine. In rats, 5-HT-induced contraction was inhibited by methysergide and by ketanserin, but not by ramosetron or tetrodotoxin. In contrast, GR113808 enhanced contractions elicited by 5-HT or 5-methoxytryptamine. These results suggest that 5-HT-induced contraction in ferrets is mediated via 5-HT1 receptors on the muscle and by release of acetylcholine via 5-HT3 receptors. In piglets, 5-HT-induced contraction appears to be mediated by release of neurotransmitters other than acetylcholine via 5-HT1 receptors. 5-HT-induced contraction in rats is evoked via 5-HT1 and 5-HT2 receptors on the muscle. Furthermore, 5-HT4 receptors may participate in the relaxation elicited by 5-HT in rats.Keywords: Species difference, 5-HT receptor, Distal ileum, Longitudinal muscle 5-Hydroxytryptamine (5-HT) is known to be distri buted in blood platelets, the nervous system and entero chromaffin cells. In the gastrointestinal tract, 5-HT is reported to contribute to the regulation of the motility and secretion via several 5-HT-receptor subtypes. The in volvement of the 5-HT-receptor subtype in 5-HT-induced intestinal contraction has been well-examined in the guinea pig small and large intestines (1-8). In contrast, the effect of 5-HT on the regulation of intestinal motility has not been established in species other than guinea pigs.Defecation and intestinal transit in rats are frequently utilized as an in vivo system for the evaluation of the ac tion of 5-HT on the motility of the small and large intes tine. However, the involvement of 5-HT receptor sub types in 5-HT-elicited responses has been less extensively investigated in vitro by the use of selective 5-HT receptor antagonists in rat isolated intestine. In the present study, we examined the participation of 5-HT1-, 5-HT2-, 5-HT3 and 5-HT4-receptor subtypes in 5-HT-induced contrac tion in rat isolated distal ileum.Since the ferret and piglet have been shown to have similarities with respect to anatomical and physiological characteristics of the gut to that of humans (9, 10), the intestinal responses in ferret and piglet are suggested to be good models of some of the physiologic and pharma cologic processes in humans. Furthermore, 5-HT-con ta...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: -Hydroxytryptaminementioning

confidence: 99%

Species Differences in the 5-Hydroxytryptamine-Induced Contraction in the Isolated Distal Ileum.

Yamano¹,

Ito²,

Miyata³

1997

Jpn.J.Pharmacol

View full text Add to dashboard Cite

show abstract

“…2). In the 9 rectum, 5-HT caused contraction (70% of rats) which was likewise dose dependent ( Fig. 1).…”

Section: Effects Of 5-ht and Tryptaminementioning

confidence: 68%

Effects of serotonin on the internal anal sphincter: in vivo manometric study in rats.

Goldberg¹,

Hanani²,

Nissan³

1986

Gut

View full text Add to dashboard Cite

SUMMARY The effects of serotonin (5-hydroxytryptamine, 5-HT) on the internal anal sphincter were studied in anaesthesised rats. Serotonin induced a dose dependent relaxation of the internal anal sphincter. Methysergide blocked this relaxation, but did not affect the rectoanal reflex. Methysergide did not antagonise the actions of cholinergic and adrenergic agonists on the internal anal sphincter. Other 5-HT antagonists such as cyproheptadine, ketanserin, chlorpromazine, amitriptyline and ergotamine failed to reduce or block the internal anal sphincter relaxation due to 5-HT, nor did they alter the rectoanal reflex. Adrenergic and cholinergic antagonists had no effect on the 5-HT-induced relaxation of the internal anal sphincter, suggesting that 5-HT acts upon the internal anal sphincter via a non-adrenergic, non-cholinergic mechanism. Tetrodotoxin reduced or blocked the relaxation of the internal anal sphincter produced by 5-HT, implying that 5-HT acts through neural pathways rather than directly on the muscle. It is concluded that although 5-HT relaxes the internal anal sphincter, it does not participate in the rectoanal reflex. Serotonin also activates non-adrenergic, noncholinergic intrinsic inhibitory neurones, and is therefore able to relax the gut if excitatory neuromuscular transmission is blocked.8 These results suggest that the role of 5-HT in the enteric nervous system is to serve as the transmitter of interneurones. reported that 5-HT relaxed the internal anal sphincter muscle strip of the vervet monkey in organ bath.Serotonin has been implicated in disturbances of gastrointestinal motility in patients with carcinoid syndrome. In a study of patients with Hirschsprung's disease, Rogawski et a113 reported the absence of serotonergic neurones in the aganglionic colon.The pharmacology of the internal anal sphincter has been studied in vivo using animal models.12 14No information is available, however, on the actions of 5-HT on the internal anal sphincter in vivo.

show abstract

“…This discrepancy could be due to the fact that histamine, when tested on rabbit and rat tissues, was devoid of a contractile effect; hence non-histaminergic stimulant factors in the extract were unmasked in these smooth muscle preparations. Histamine is known to be devoid of a spasmogenic potential in rabbit intestinal (Botting, 1975) and rat gastric (Matuszek et al, 1992;1994), intestinal (Sakai et al, 1979) and uterine tissues (Bertaccini et al, 1979). It is also known that the guineapig is an ideal model for studying histaminergic mechanisms (Levi et al, 1982) while tissues from rat and rabbit in comparison with the guinea-pig are less sensitive to ligands acting at the histaminergic receptors (McLeod et al, 1994).…”

Section: Discussionmentioning

confidence: 97%

Gastrointestinal stimulatory and uterotonic activities of dietary radish leaves extract are mediated through multiple pathways

Ghayur

Gilani

2005

Phytother. Res.

View full text Add to dashboard Cite

Raphanus sativus, commonly known as radish, is a food plant known worldwide for its culinary and medicinal properties especially as a laxative and abortifacient. This study reports the gastrointestinal and uterine tone modulatory activities of the crude extract (Rl.Cr) of radish leaves. Rl.Cr, showing the presence of saponins and alkaloids, exhibited a spasmogenic effect (0.03-10 mg/mL) in isolated rabbit jejunum, rat stomach fundus and uterus which was partially blocked by atropine. In contrast, Rl.Cr was found to be devoid of any stimulatory effect in rat ileum, instead showed an inhibitory effect (0.1 mg/mL) on the ACh dose-response curves. A mild relaxant effect was also observed in rabbit jejunum at the lower doses (0.1-0.3 mg/mL) but not against K(+)-induced contractions, ruling out a calcium channel blocking effect. In guinea-pig ileum, Rl.Cr exhibited a stimulant effect resistant to atropine while sensitive to pyrilamine pretreatment. The aqueous fraction, showing a strong presence of saponins, was found to be more efficacious than the non-polar fractions in its spasmogenic effect. This study shows the presence of species-dependent gastrointestinal effects of radish mediated partially through cholinergic receptors in rabbit and rat tissues, but through histaminergic activation in the guinea-pig, providing a scientific basis for its use in gut and uterine affections while also giving a wider picture of the activity profile of radish by using different species of animals.

show abstract

THE ACTIONS OF 5‐HYDROXYTRYPTAMINE AND HISTAMINE ON THE ISOLATED ILEUM OF THE TREE SHREW (Tupaia glis)

Cited by 22 publications

References 12 publications

Species Differences in the 5-Hydroxytryptamine-Induced Contraction in the Isolated Distal Ileum.

Species Differences in the 5-Hydroxytryptamine-Induced Contraction in the Isolated Distal Ileum.

Effects of serotonin on the internal anal sphincter: in vivo manometric study in rats.

Gastrointestinal stimulatory and uterotonic activities of dietary radish leaves extract are mediated through multiple pathways

Contact Info

Product

Resources

About