The actions of calcitonin gene related peptide and vasoactive intestinal peptide as vasodilators in man in vivo and in vitro.

Thom, S; Hughes, Alun D.; Goldberg, P; Martin, Gladys; Schächter, M; Sever, PS

doi:10.1111/j.1365-2125.1987.tb03154.x

Cited by 78 publications

(25 citation statements)

References 29 publications

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“…In rings of rat aorta and mesenteric artery, human a-CGRP acts in an endothelial dependent manner like acetylcholine (Brain et al, 1985;Kubota et al, 1985;Al-Kazwini et al, 1987). In human radial, coronary and gastric arteries the relaxation evoked by human a-CGRP was also endothelium dependent (Thom et al, 1987). In contrast to these observations, in rings of cat middle cerebral artery the response was independent of the endothelium (Hanko et al, 1985).…”

Section: Discussioncontrasting

confidence: 48%

Human alpha‐calcitonin gene‐related peptide (CGRP) is a potent vasodilator in human mesenteric vasculature.

Marshall¹,

Al-Kazwini²,

Holman³

et al. 1988

Brit J Clinical Pharma

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Section: Discussioncontrasting

confidence: 48%

Human alpha‐calcitonin gene‐related peptide (CGRP) is a potent vasodilator in human mesenteric vasculature.

Marshall¹,

Al-Kazwini²,

Holman³

et al. 1988

Brit J Clinical Pharma

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“…In the original description of its cardiovascular activity (Brain et al, 1985), vasodilatation induced by CGRP in the rat aorta was endothelium-dependent, and similar findings were obtained with human vessels (Thom et al, 1987). However, in the bovine coronary artery vasodilatation in response to CGRP did not require the presence of endothelium (Greenberg et al, 1987).…”

Section: Introductionsupporting

confidence: 54%

Action of calcitonin gene‐related peptide upon bovine vascular endothelial and smooth muscle cells grown in isolation and co‐culture

Crossman¹,

Dashwood²,

Brain³

et al. 1990

British J Pharmacology

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1 Bovine aortic endothelial cells (BAE) and smooth muscle cells (BASM) were grown separately and in co-culture. 2 Calcitonin gene-related peptide (CGRP) caused dose-dependent activation of adenylate cyclase in each cell type when grown in isolation. The concentration of CGRP causing half-maximal activation in BAE and BASM was 200nM and 310nm, respectively. 3 In cells grown in co-culture exposure to bradykinin produced dose-dependent elevations in cyclic GMP content which were maximal 1 min after application of the agonist.4 CGRP (1 nM-i gM) did not stimulate a rise in cyclic GMP in co-cultures.5 Displaceable CGRP binding was identified throughout the wall of the bovine aorta. 6 We conclude that CGRP receptors coupled to adenylate cyclase are present on BAE and BASM, but there is no coupling of these receptors to the release of any agent (such as endothelium-derived relaxing factor) that activates guanylate cyclase.

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“…The vasodilator effect of calcitonin gene-related peptide (CGRP) (Amara et al, 1982) has been extensively studied in the coronary circulation of several mammals Greenberg et al, 1987;Shoji et al, 1987;Franco-Cereceda, 1988;Beny et al, 1989) including man (McEwan et al, 1986;Thom et al, 1987). CGRP-immunoreactivity is present in high concentration in cardiac tissue and in nerve fibres associated with coronary blood vessels (Mulderry et al, 1985).…”

Section: Introduction Methodsmentioning

confidence: 99%

Heterogeneous involvement of endothelium in calcitonin gene‐related peptide‐induced relaxation in coronary arteries from rat

Prieto

Benedito

Nyborg

1991

British J Pharmacology

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1 The effects of rat-and human-CGRP and capsaicin were studied in isolated rings of rat proximal epicardial (PC) and distal intramyocardial (DC) coronary arteries. 2 The relaxing effect of rat-CGRP was dependent on the level of vessel tone induced by prostaglandin F2a (PGF20) in PC but not in DC arteries. Submaximally contracted DC and PC arteries were more sensitive to rat-than human-CGRP. There was no difference in sensitivity to rat-and human-CGRP between PC and DC arteries. 3 Substance P elicited a small relaxation only in 4 of the 6 PC arteries tested. PC and DC arteries were concentration-dependently relaxed by capsaicin. The relaxation was partly inhibited by ruthenium red, thus suggesting that capsaicin causes specific release of CGRP from sensory nerve endings in rat coronary arteries. 4 The relaxant effect of rat-CGRP was antagonized by endothelium removal and indomethacin but not methylene blue in endothelium-intact PC arteries. The relaxation in DC arteries was not affected by any of these treatments, indicating a heterogeneous involvement of the endothelium in CGRP-mediated coronary vasodilatation and the release of a cyclo-oxygenase product in PC arteries in rats. 5 Glibenclamide had no inhibitory effect on the CGRP-induced relaxation of PC and DC arteries, thus excluding the involvement of glibenclamide-sensitive K+-channels in the mechanism of action of CGRP in rat coronary arteries.

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The actions of calcitonin gene related peptide and vasoactive intestinal peptide as vasodilators in man in vivo and in vitro.

Cited by 78 publications

References 29 publications

Human alpha‐calcitonin gene‐related peptide (CGRP) is a potent vasodilator in human mesenteric vasculature.

Human alpha‐calcitonin gene‐related peptide (CGRP) is a potent vasodilator in human mesenteric vasculature.

Action of calcitonin gene‐related peptide upon bovine vascular endothelial and smooth muscle cells grown in isolation and co‐culture

Heterogeneous involvement of endothelium in calcitonin gene‐related peptide‐induced relaxation in coronary arteries from rat

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