The activation of K-ras gene at an early stage of lung tumorigenesis in mice

Ichikawa, Tomio; Yano, Yoshihisa; Uchida, Mikako; Otani, Shuzo; Hagiwara, Kiyokazu; Yano, Tomohiro

doi:10.1016/0304-3835(96)04351-0

Cited by 25 publications

(15 citation statements)

References 9 publications

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“…More specifically, if we further dissect these results by analysing both genes separately, we saw that while p16 INK4A inactivation was more frequently recognised at an advanced tumor stage, K-ras did not, as it was more frequently detected in tumors at an early stage. These findings are in keeping with studies of Ng et al [38] showing a correlation between p16 INK4A methylation and an advanced tumor stage and with the studies of Mao et al [10], Graziano et al [39], Ichikawa et al [40] and Yano et al [41] showing K-ras mutations at an early stage of lung carcinogenesis.…”

Section: Discussionsupporting

confidence: 93%

K-ras and p16INK4Aalterations in sputum of NSCLC patients and in heavy asymptomatic chronic smokers

et al. 2004

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Section: Discussionsupporting

confidence: 93%

K-ras and p16INK4Aalterations in sputum of NSCLC patients and in heavy asymptomatic chronic smokers

et al. 2004

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“…From these results it may be argued that KRAS mutations are a very early event in carcinogenesis of pulmonary adenocarcinomas and this is in agreement with animal model studies suggesting that KRAS mutations occur early in lung tumour [16][17][18][19][20][21].…”

Section: Discussionsupporting

confidence: 84%

Smoking history and lung carcinoma: KRAS mutation is an early hit in lung adenocarcinoma development

et al. 2012

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“…the maSa method is one of the most highly sensitive methods to detect mutation alleles in the presence of a very large excess of normal alleles (6)(7)(8). it is based on the inability of taq dna polymerase to elongate a primer when the 3' end does not match the template dna.…”

Section: Discussionmentioning

confidence: 99%

Sensitive quantitative assay for point mutations in the rat H-ras gene based on single nucleotide primer extension

Yano

Yano²,

Kinoshita³

et al. 2010

Experimental and Therapeutic Medicine

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Abstract. point mutations in oncogenes and tumor suppressor genes occur at early stages in the carcinogenic process. point mutations in ras family oncogenes are the most common mutational events in several types of human cancer, and are available as molecular markers for the detection of cancer cells in carcinogenicity bioassay systems as well as in clinical samples. although several techniques are utilized to detect point mutations in carcinogenicity bioassay systems, the sensitivity is too low to determine a small number of mutations. in order to overcome the disadvantage and to sensitively determine gene mutation rates for in vivo carcinogenicity bioassays of presumptive carcinogens, we established a thermosequenase cycle End labeling (tcEl) method, a sensitive approach based on single nucleotide primer extension. One of the characteristics of the method is a high sensitivity of 1:100,000, ten times the sensitivity of the mutant allele-specific amplification now commonly employed. Using TCEL, we here quantified h-ras mutations in the livers of rats treated with a genotoxic carcinogen, 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. Our findings suggest that this method may be applied for many genetic targets as a component in vivo.

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The activation of K-ras gene at an early stage of lung tumorigenesis in mice

Cited by 25 publications

References 9 publications

K-ras and p16INK4Aalterations in sputum of NSCLC patients and in heavy asymptomatic chronic smokers

K-ras and p16INK4Aalterations in sputum of NSCLC patients and in heavy asymptomatic chronic smokers

Smoking history and lung carcinoma: KRAS mutation is an early hit in lung adenocarcinoma development

Sensitive quantitative assay for point mutations in the rat H-ras gene based on single nucleotide primer extension

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