The Activin-like ligand Dawdle regulates innate immune responses through modulating NF-κB signaling in mud crab Scylla paramamosain

Zhou, Yi; Li, Bo; Xu, Ya Ping; Wang, Lan Zhi; Gu, Wen Bin; Liu, Ze Peng; Wang, Dong; Miao, Shuying

doi:10.1016/j.dci.2019.103450

Cited by 9 publications

(3 citation statements)

References 33 publications

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“…Recently, a cDNA encoding a Dawdle-like factor was characterized in S. paramansosain . Sp-Daw is expressed primarily in embryos and larvae, suggesting that it plays a role in developmental processes ( 153 ). It may also be involved in the innate immune response in adults ( 153 ).…”

Section: Transforming Factor β/Activin Signalingmentioning

confidence: 99%

Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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A pair of Y-organs (YOs) are the molting glands of decapod crustaceans. They synthesize and secrete steroid molting hormones (ecdysteroids) and their activity is controlled by external and internal signals. The YO transitions through four physiological states over the molt cycle, which are mediated by molt-inhibiting hormone (MIH; basal state), mechanistic Target of Rapamycin Complex 1 (mTORC1; activated state), Transforming Growth Factor-β (TGFβ)/Activin (committed state), and ecdysteroid (repressed state) signaling pathways. MIH, produced in the eyestalk X-organ/sinus gland complex, inhibits the synthesis of ecdysteroids. A model for MIH signaling is organized into a cAMP/Ca2+-dependent triggering phase and a nitric oxide/cGMP-dependent summation phase, which maintains the YO in the basal state during intermolt. A reduction in MIH release triggers YO activation, which requires mTORC1-dependent protein synthesis, followed by mTORC1-dependent gene expression. TGFβ/Activin signaling is required for YO commitment in mid-premolt. The YO transcriptome has 878 unique contigs assigned to 23 KEGG signaling pathways, 478 of which are differentially expressed over the molt cycle. Ninety-nine contigs encode G protein-coupled receptors (GPCRs), 65 of which bind a variety of neuropeptides and biogenic amines. Among these are putative receptors for MIH/crustacean hyperglycemic hormone neuropeptides, corazonin, relaxin, serotonin, octopamine, dopamine, allatostatins, Bursicon, ecdysis-triggering hormone (ETH), CCHamide, FMRFamide, and proctolin. Contigs encoding receptor tyrosine kinase insulin-like receptor, epidermal growth factor (EGF) receptor, and fibroblast growth factor (FGF) receptor and ligands EGF and FGF suggest that the YO is positively regulated by insulin-like peptides and growth factors. Future research should focus on the interactions of signaling pathways that integrate physiological status with environmental cues for molt control.

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Section: Transforming Factor β/Activin Signalingmentioning

confidence: 99%

Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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“…In crustaceans, the TGF-β superfamily members display multifunctional characteristics in regulating growth, metabolism, immune response, and appendage regeneration ( Klinbunga et al, 2018 ; Zhou et al, 2018 ; Shinji et al, 2019 ; Zhou et al, 2019 ). In particular, myostatin, which is a negative growth regulator in vertebrates, is involved in complex and potentially multimodal actions in crustaceans ( Lee et al, 2015 ; Mykles & Medler, 2015 ; Abuhagr et al, 2016 ; Zhuo et al, 2017 ; Yue et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis

Wang

Zhang

Hou

et al. 2020

PeerJ

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Activin receptor IIB (ActRIIB) is a serine/threonine-kinase receptor binding with transforming growth factor-β (TGF-β) superfamily ligands to participate in the regulation of muscle mass in vertebrates. However, its structure and function in crustaceans remain unknown. In this study, the ActRIIB gene in Eriocheir sinensis (Es-ActRIIB) was cloned and obtained with a 1,683 bp open reading frame, which contains the characteristic domains of TGF-β type II receptor superfamily, encoding 560 amino acids. The mRNA expression of Es-ActRIIB was the highest in hepatopancreas and the lowest in muscle at each molting stage. After injection of Es-ActRIIB double-stranded RNA during one molting cycle, the RNA interference (RNAi) group showed higher weight gain rate, higher specific growth rate, and lower hepatopancreas index compared with the control group. Meanwhile, the RNAi group displayed a significantly increased content of hydrolytic amino acid in both hepatopancreas and muscle. The RNAi group also displayed slightly higher contents of saturated fatty acid and monounsaturated fatty acid but significantly decreased levels of polyunsaturated fatty acid compared with the control group. After RNAi on Es-ActRIIB, the mRNA expressions of five ActRIIB signaling pathway genes showed that ActRI and forkhead box O (FoxO) were downregulated in hepatopancreas and muscle, but no significant expression differences were found in small mother against decapentaplegic (SMAD) 3, SMAD4 and mammalian target of rapamycin. The mRNA expression s of three lipid metabolism-related genes (carnitine palmitoyltransferase 1β (CPT1β), fatty acid synthase, and fatty acid elongation) were significantly downregulated in both hepatopancreas and muscle with the exception of CPT1β in muscles. These results indicate that ActRIIB is a functionally conservative negative regulator in growth mass, and protein and lipid metabolism could be affected by inhibiting ActRIIB signaling in crustacean.

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“…In crustaceans, the TGF-β superfamily members display multifunctional characteristics in regulating growth, metabolism, immune response, and appendage regeneration (Klinbunga et al, 2018;Shinji et al, 2019;Zhou et al, 2019). In particular, myostatin, which is a negative growth regulator in vertebrates, is involved in complex and potentially multimodal actions in crustaceans (Lee et al, 2015;Mykles & Medler, 2015;Abuhagr et al, 2016;Zhuo et al, 2017;Yue et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis (v0.1)"

2020

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Activin receptor IIB (ActRIIB) is a serine/threonine-kinase receptor binding with transforming growth factor-β (TGF-β) superfamily ligands to participate in the regulation of muscle mass in vertebrates. However, its structure and function in crustaceans remain unknown. In this study, the ActRIIB gene in Eriocheir sinensis (Es-ActRIIB) was cloned and obtained with a 1683 bp open reading frame, which contains the characteristic domains of TGF-β type II receptor superfamily, encoding 560 amino acids. The mRNA expression of Es-ActRIIB was the highest in hepatopancreas and the lowest in muscle at each molting stage. After injection of Es-ActRIIB double-stranded RNA during one molting cycle, the RNA interference (RNAi) group showed higher weight gain rate, higher specific growth rate, and lower hepatopancreas index compared with the control group. Meanwhile, the RNAi group displayed a significantly increased content of hydrolytic amino acid in both hepatopancreas and muscle. The RNAi group also displayed slightly higher contents of saturated fatty acid and monounsaturated fatty acid but significantly decreased levels of polyunsaturated fatty acid compared with the control group. After RNAi on Es-ActRIIB, the mRNA expressions of five ActRIIB signaling pathway genes showed that ActRI and forkhead box O (FoxO) were downregulated in hepatopancreas and muscle, but no significance expression differences were found in small mother against decapentaplegic (SMAD) 3, SMAD4 and mammalian target of rapamycin. The mRNA expression s of three lipid metabolism-related genes (carnitine palmitoyltransferase 1β (CPT1β), fatty acid synthase, and fatty acid elongation) were significantly downregulated in both hepatopancreas and muscle with the exception of CPT1β in muscles. These results indicate that ActRIIB is a functionally conservative negative regulator in growth mass, and protein and lipid metabolism could be affected by inhibiting ActRIIB signaling in crustacean.

show abstract

The Activin-like ligand Dawdle regulates innate immune responses through modulating NF-κB signaling in mud crab Scylla paramamosain

Cited by 9 publications

References 33 publications

Signaling Pathways That Regulate the Crustacean Molting Gland

Signaling Pathways That Regulate the Crustacean Molting Gland

Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis

Peer Review #1 of "Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis (v0.1)"

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