The Activities of the Aqueous Crude Extracts of the Leaf, Root and Bark of Combretum Molle Against Selected Test Organisms

Kulawe, D.; Abubakar, AS; Abubakar, Z.

doi:10.29322/ijsrp.9.10.2019.p9428

Cited by 2 publications

(3 citation statements)

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“…Penelitian ini ingin mengetahui efektivitas dari Ekstrak daun ketapang (Terminalia catappa L.) dengan larutan metanol 98% yang akan diujikan pada bakteri patogen ikan lele (Clarisa batrachus L.). Ekstrak Daun ketapang diperoleh dengan menggunakan metode maserasi (Kulawe et al, 2019).…”

Section: Metodeunclassified

Potency of Ketapang (Terminalia catappa L.) Leaves Methanol Extract Against Pathogenic Bacteria of Catfish (Clarias batrachus L.)

Wibowo

Darwis²,

Sipriyadi³

et al. 2022

JPBN

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Ikan lele (Clarias batrachus L.) merupakan salah satu spesies penting sebagai komoditas air tawar di Indonesia dengan produksi yang terus meningkat setiap tahunnya. Namun, ikan lele rentan terhadap beberapa infeksi bakteri, terutama bila dipelihara dalam kondisi kepadatan tinggi, salah satunya oleh bakteri patogen Bacillus cereus. Banyak sumber bahan baku lokal yang dapat dimanfaatkan sebagai pakan ikan untuk mengatasi masalah tersebut dan mengatasi penyakit yang muncul pada budidaya ikan, salah satunya adalah tanaman tradisional ketapang (Terminalia catappa L.) yang merupakan tanaman yang memiliki potensi sebagai zat antibakteri. Tujuan penelitian ini untuk mengetahui dan menguji aktivitas antibakteri ekstrak daun ketapang (T. catappa L.) terhadap bakteri patogen B. cereus dari isolat hati ikan lele (Clarias batrachus L.) penyebab penyakit pada budidaya. Metode yang digunakan yaitu maserasi dengan pelarut metanol perbandingan 1:10 yang dimaserasi 7 x 24 jam. Uji aktivitas antibakteri dengan metode cakram difusi dengan konsentrasi 45%, 52,5%, 60%, 67,5%, dan 75%. Hasil uji aktivitas antibakteri ekstrak daun ketapang mampu menghambat pertumbuhan bakteri B. cereus pada konsentrasi 45% dengan rata-rata diameter zona hambat sebesar 13,51 mm (kategori kuat). Dari hasil yang diperoleh, dapat disimpulkan bahwa ekstrak daun ketapang memiliki kemampuan dalam menghambat pertumbuhan bakteri patogen B. cereus penyebab penyakit pada hati ikan lele (C. batrachus L.)

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Section: Metodeunclassified

Potency of Ketapang (Terminalia catappa L.) Leaves Methanol Extract Against Pathogenic Bacteria of Catfish (Clarias batrachus L.)

Wibowo

Darwis²,

Sipriyadi³

et al. 2022

JPBN

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“…The inocula of the test organisms were standardized by the method of Bauer and Thornsberry [14]. This was done by suspending each test organism in 5ml of nutrient broth and the turbidity was compared with that of 0.5 McFarland standard [15]. McFarland standard was prepared by adding 0.6 ml of 1% barium chloride (BaCl 2 ) to 99.4 ml of 1% sulphuric acid (H 2 S0 4 ) solution.…”

Section: Source and Preparation Of Test Microorganismsmentioning

confidence: 99%

“…The positive control performed better than the extracts in terms of zone of inhibition. The results of the minimum Kulawe et al; AJB2T, 5(4): 1-7, 2019; Article no.AJB2T.52124 2 inhibitory concentration (MIC) indicated that the leaf ethanolic extract had the greatest activity against K. pneumoniae with MIC value of 12.5 mg/ml, while the results from the minimum bactericidal concentration (MBC) showed that the leaf ethanolic extract had the greatest activity against K. pneumoniae with an MBC value of 12.5 mg/ml.…”

mentioning

confidence: 99%

The Ethanolic Extracts of the Root, Stem and Leaves of Balanites aegyptiaca against Selected Microbes

Kulawe

Ahidjo

Hena³

2019

AJB2T

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Balanites aegyptiaca has been used in many traditional treatments of microbial infections. This work was carried out with the aim of determining the phytochemical compounds present in the ethanolic extracts of the root, stem and leaves of Balanites aegyptiaca and their effects against selected microorganisms. Phytochemical screening was undertaken to determine presence of secondary metabolites in the stem-bark, roots and leaves of the test plant. Well diffusion method was used to determine sensitivity of test organisms to the test plant. Result indicated the presence of alkaloids, tannins, flavonoids, saponins, steroids, and anthraquinones, although anthraquinones were absent in ethanolic extracts of root and stem bark of Balanites aegyptiaca but present in the ethanolic extract of the leaf. Antibacterial effects were tested against Staphylococcus aureus and Klebsiella pneumoniae. Results obtained from the ethanolic extract of the root stem and leaves showed significant zones of inhibition against Klebsiella pneumoniae and Staphylococcus aureus at all concentrations of 100 mg/ml, 50 mg/ml, 25 mg/ml, 12.5 mg/ml and 6.125 mg/ml. The positive control performed better than the extracts in terms of zone of inhibition. The results of the minimum Kulawe et al.; AJB2T, 5(4): 1-7, 2019; Article no.AJB2T.52124 2 inhibitory concentration (MIC) indicated that the leaf ethanolic extract had the greatest activity against K. pneumoniae with MIC value of 12.5 mg/ml, while the results from the minimum bactericidal concentration (MBC) showed that the leaf ethanolic extract had the greatest activity against K. pneumoniae with an MBC value of 12.5 mg/ml. Original Research Article

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The Activities of the Aqueous Crude Extracts of the Leaf, Root and Bark of Combretum Molle Against Selected Test Organisms

Cited by 2 publications

References 0 publications

Potency of Ketapang (Terminalia catappa L.) Leaves Methanol Extract Against Pathogenic Bacteria of Catfish (Clarias batrachus L.)

Potency of Ketapang (Terminalia catappa L.) Leaves Methanol Extract Against Pathogenic Bacteria of Catfish (Clarias batrachus L.)

The Ethanolic Extracts of the Root, Stem and Leaves of Balanites aegyptiaca against Selected Microbes

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