The activities of two Ets-related transcription factors required for drosophila eye development are modulated by the Ras/MAPK pathway

O’Neill, Elizabeth; Rebay, Ilaria; Tjian, Robert; Rubin, Gerald M.

doi:10.1016/0092-8674(94)90580-0

Cited by 673 publications

(624 citation statements)

References 33 publications

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“…Recent data has shown that ETS1 and ETS2 require phosphorylation of a speci®c residue in the Nterminal domain by the MAP kinase pathway for full transactivational activity (Yang et al, 1996). This phosphorylation site found in ETS1 and ETS2 is conserved with Drosophila ETS family member pointed, which plays a critical role in photoreceptor downstream of the tyrosine kinase receptor sevenless (O'Neill et al, 1994). ELF3 does not appear to have the pointed phosphorylation domain but does contain a number of consensus phosphorylation sites for Figure 8 Transactivation by ELF3.…”

Section: Discussionmentioning

confidence: 99%

“…In Drosophila, ETS family members play important developmental roles: E74 plays a role in early gene regulation following ecdysone triggering (Burtis et al, 1990;Fletcher et al, 1995); pointed P2 and yan play critical roles in eye development (O'Neill et al, 1994); and D-elg plays a role in developmental programming (Schulz et al, 1993). In humans and mice ETS proteins may function in a wide range of biological processes such as hematopoiesis (Scott et al, 1994a;Muthusamy et al, 1995), organogenesis and branching morphogenesis (Kola et al, 1993), and skeletal/cartilage development (Sumarsono et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer

et al. 1997

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The ETS family of genes are implicated in cancers such as Ewings sarcoma, acute myeloid leukemia and chronic myelomoncytic leukemia. Further, they have important functions in embryonic development. Hence, identi®ca-tion and characterization of members of this family are important. We identify a novel ETS family member, ELF3, and report its human and murine cDNA sequences. The mouse cDNA has an alternatively spliced transcript with an extra 60 bp inserted. Hence we present the organization of the murine Elf3 gene together with its exon/intron structure. This gene consists of 9 exons and 8 introns spanning 4.8 kb. ELF3 binds and transactivates ETS sequences and interestingly also shows the ability to bind a GGAT-like purine core, a preferential ETS1/ETS2 type binding site. The expression of ELF3, unlike most other ETS family members, is absent in hematopoietic cells and hematopoietic organs in humans and mice. Intriguingly, the gene is speci®cally expressed in cell lines of epithelial origin and in organs such as lung, stomach, intestine, kidney that have specialized epithelial cells. We localize the human gene to 1q32.2, a region that is ampli®ed in epithelial tumors of the breast, lung and prostate. Finally, we show that ELF3 expression is increased in a lung carcinoma and adenocarcinoma, as compared to normal tissue. ELF3 is also expressed in cell lines derived from lung cancers. These results suggest that this novel ETS gene may be involved in lung tumorigenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer

et al. 1997

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“…Additional characteristics, such as the MAPK sites within PEA3 [21], the phosphorylation site within Ets-1 [22], or the Pointed (PNT) domain (a protein-binding domain) in Ets-1 [23], aid in target gene recognition and/or protein-protein interactions.…”

Section: Introductionmentioning

confidence: 99%

Epithelium-specific ets transcription factor 2 upregulates cytokeratin 18 expression in pulmonary epithelial cells through an interaction with cytokeratin 18 intron 1

YANIW,

2005

Cell Res

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The role of Ese-2, an Ets family transcription factor, in gene regulation is not known. In this study, the interaction between Ese-2 and cytokeratin 18 (K18) intron 1 was characterized in lung epithelial cells. Reporter gene assays showed Ese-2 was able to upregulate K18 intron 1 enhanced reporter gene expression by approximately 2-fold. We found that full length Ese-2 did not bind DNA strongly, therefore truncated versions of the protein, containing the ETS domain or Pointed domain, were created and tested in electrophoresis mobility shift assays. Multiple interactions between the ETS domain and putative DNA binding sites within K18 intron 1 were observed, which led to the determination of a possible Ese-2 DNA binding consensus sequence. These experiments suggest that Ese-2 could play a role in the regulation of K18 expression in lung epithelial cells.

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“…Pointed P2 and yan play critical roles in Drosophila eye development (O'Neill et al, 1994). ETS2 is involved in skeletal/cartilage development (Sumarsono et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

A novel transcription factor, ELF5, belongs to the ELF subfamily of ETS genes and maps to human chromosome 11p13–15, a region subject to LOH and rearrangement in human carcinoma cell lines

Zhou

Tymms

et al. 1998

Oncogene

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The ETS transcription factors are a large family implicated in the control of cellular proliferation and tumorigenesis. In addition, chromosomal translocations involving ETS family members are associated with a range of di erent human cancers. Given the extensive involvement of ETS factors in tumorigenesis, it becomes important to identify any additional ETS genes that may also play oncogenic roles. We identify a novel gene, ELF5, that appears to belong to the ELF (E74-likefactor) subfamily of the ETS transcription factor family, based upon similarity within the`ETS domain'. ELF5 displays a similar, but more restricted, expression pattern to that of the newly isolated epithelium-speci®c ETS gene, ELF3. Unlike most other ETS family members, ELF5 is not expressed in hematopoietic compartments, but is restricted to organs such as lung, stomach, kidney, prostate, bladder and mammary gland. ELF5 is localized to human chromosome 11p13 ± 15, a region that frequently undergoes loss of heterozygosity (LOH) in several types of carcinoma, including those of breast, kidney and prostate. We ®nd that ELF5 expression is not detectable in a number of carcinoma cell lines, some of which display loss or rearrangement of an ELF5 allele. Similar to other ETS family members, ELF5 displays speci®c binding to DNA sequences containing a GGAA-core. In addition, ELF5 is able to transactivate through these ETS sequences, present upstream from a minimal promoter. Our data suggest that ELF5 may play roles in mammary, lung, prostate and/or kidney function, and possibly also in tumorigenesis.

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The activities of two Ets-related transcription factors required for drosophila eye development are modulated by the Ras/MAPK pathway

Cited by 673 publications

References 33 publications

A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer

A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer

Epithelium-specific ets transcription factor 2 upregulates cytokeratin 18 expression in pulmonary epithelial cells through an interaction with cytokeratin 18 intron 1

A novel transcription factor, ELF5, belongs to the ELF subfamily of ETS genes and maps to human chromosome 11p13–15, a region subject to LOH and rearrangement in human carcinoma cell lines

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