The activity of class I, II, III and IV alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in renal cell carcinoma

Orywal, Karolina; Jelski, Wojciech; Werel, Tadeusz; Szmitkowski, Maciej

doi:10.1016/j.yexmp.2015.03.012

Cited by 16 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both the serum and tissue expression level of class I and total ADH were significantly increased in liver,27,28 colorectal,29,30 and brain cancers,31,32 while ALDH levels were not statistically significant different between cancer patients and healthy subjects. The upregulation trend of class I ADH between cancer and healthy tissue can also be found in cervical,33 ovarian,34 endometrial,35 and renal cell cancers,36 whereas the upregulation trend of class I ADH for cancer serum has been reported in bladder and esophageal cancers 37,38. However, the expression of class I ADH in breast cancer was significantly decreased compared to the tissue of healthy subjects, and the expression of ALDH was unchanged 39.…”

Section: Discussionmentioning

confidence: 95%

Distinct prognostic values of alcohol dehydrogenase mRNA expression in pancreatic adenocarcinoma

Liao¹,

Huang²,

Liu³

et al. 2017

OTT

View full text Add to dashboard Cite

BackgroundAlcohol dehydrogenase (ADH) isoenzymes have been reported as a potential diagnostic marker for pancreatic cancer, but their prognostic value in pancreatic cancer remains unclear. The aim of this investigation was to identify the prognostic value of ADH genes in human patients with pancreatic adenocarcinoma (PAAD).Materials and methodsAn RNA sequencing dataset and corresponding survival profiles of PAAD were obtained from The Cancer Genome Atlas. Survival analysis and gene set enrichment analysis were used to investigate the prediction value and potential mechanism of ADH genes in PAAD prognosis.ResultsSurvival analysis of ADH genes suggests that a high expression of ADH1A (adjusted P=0.037, adjusted hazard ratio [HR] =0.627, 95% CI =0.404–0.972) and ADH6 (adjusted P=0.018, adjusted HR =0.588, 95% CI =0.378–0.914) were associated with a significantly decreased risk of death, while a high expression of ADH5 was associated with a significantly increased risk of death (adjusted P=0.043, adjusted HR =1.564, 95% CI =1.013–2.414). Joint effects analysis of three ADH gene prognostic markers suggests that the prognosis difference for any marker combination was more significant than that for any individual marker. The potential mechanism of ADH1A and ADH6 in PAAD prognosis was that a high expression of ADH1A and ADH6 was involved in the P450 pathway and biological processes, while high ADH5 expression was involved in transforming growth factor β regulation-related pathways and biological processes, Wnt, the cell cycle, ErbB, and mitogen-activated protein kinase signaling pathways.ConclusionOur data suggest that ADH1A, ADH5, and ADH6 expression may be potential prognostic markers of PAAD and in combination have a strong interaction and better predictive value for PAAD prognosis.

show abstract

Section: Discussionmentioning

confidence: 95%

Distinct prognostic values of alcohol dehydrogenase mRNA expression in pancreatic adenocarcinoma

Liao¹,

Huang²,

Liu³

et al. 2017

OTT

View full text Add to dashboard Cite

show abstract

“…Similarly, as in the serum results of patients with malignant neoplasms, ADH isoenzyme activity has been identified in tumor tissues ( 37 ). Studies have additionally reported that class I ADH isoenzymes are significantly increased in the tumor tissue of cervical cancer ( 47 ), ovarian cancer ( 48 ), endometrial cancer ( 49 ), brain cancer ( 50 ), liver cancer ( 44 , 51 ), CRC ( 52 , 53 ) and renal cell carcinoma ( 54 ) compared with healthy tissue, whereas ALDH isoenzymes in these cancer types did not demonstrate a statistically significant difference between cancer and healthy tissue. However, a downregulation of class I ADH was observed in breast cancer tissues ( 55 ), whereas the expression of other ADH isoenzymes remained unchanged.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of alcohol dehydrogenase mRNA expression in gastric cancer

et al. 2018

View full text Add to dashboard Cite

Previous studies have reported that alcohol dehydrogenase (ADH) isoenzymes possess diagnostic value in gastric cancer (GC). However, the prognostic value of ADH isoenzymes in GC remains unclear. The aim of the present study was to identify the prognostic value of ADH genes in patients with GC. The prognostic value of ADH genes was investigated in patients with GC using the Kaplan-Meier plotter tool. Kaplan-Meier plots were used to assess the difference between groups of patients with GC with different prognoses. Hazard ratios (HR) and 95% confidence intervals (CI) were used to assess the relative risk of GC survival. Overall, 593 patients with GC and 7 ADH genes were included in the survival analysis. High expression of ADH 1A (class 1), α polypeptide (ADH1A; log-rank P=0.043; HR=0.79; 95% CI: 0.64–0.99), ADH 1B (class 1), β polypeptide (ADH1B; log-rank P=1.9×10−05; HR=0.65; 95% CI: 0.53–0.79) and ADH 5 (class III), χ polypeptide (ADH5; log-rank P=0.0011; HR=0.73; 95% CI: 0.6–0.88) resulted in a significantly decreased risk of mortality in all patients with GC compared with patients with low expression of those genes. Furthermore, protective effects may additionally be observed in patients with intestinal-type GC with high expression of ADH1B (log-rank P=0.031; HR=0.64; 95% CI: 0.43–0.96) and patients with diffuse-type GC with high expression of ADH1A (log-rank P=0.014; HR=0.51; 95% CI: 0.3–0.88), ADH1B (log-rank P=0.04; HR=0.53; 95% CI: 0.29–0.98), ADH 4 (class II), π polypeptide (log-rank P=0.033; HR=0.58; 95% CI: 0.35–0.96) and ADH 6 (class V) (log-rank P=0.037; HR=0.59; 95% CI: 0.35–0.97) resulting in a significantly decreased risk of mortality compared with patients with low expression of those genes. In contrast, patients with diffuse-type GC with high expression of ADH5 (log-rank P=0.044; HR=1.66; 95% CI: 1.01–2.74) were significantly correlated with a poor prognosis. The results of the present study suggest that ADH1A and ADH1B may be potential prognostic biomarkers of GC, whereas the prognostic value of other ADH genes requires further investigation.

show abstract

“…Since the amount of some lipids in cancer cells membranes is greater than in normal cells, it can be assumed that the increase in aldehyde levels in RCC cell lines may be linked to changes in the membrane lipid composition subsequent to increased oxidative stress [30][31][32]. Furthermore, it is also known that RCC cells have a significantly higher activity of total ADH and ADH class I that is exacerbated in patients with a more advanced stage [39,40], meaning that tumoral cells have an increased ability to produce aldehydes, which can intensify carcinogenesis. In agreement, a significant enhancement of aldehydes has been previously shown in urine from RCC patients compared to healthy controls [27].…”

Section: Discussionmentioning

confidence: 99%

Volatilomics Reveals Potential Biomarkers for Identification of Renal Cell Carcinoma: An In Vitro Approach

et al. 2020

View full text Add to dashboard Cite

The identification of noninvasive biomarkers able to detect renal cell carcinoma (RCC) at an early stage remains an unmet clinical need. The recognition that altered metabolism is a core hallmark of cancer boosted metabolomic studies focused in the search for cancer biomarkers. The present work aims to evaluate the performance of the volatile metabolites present in the extracellular medium to discriminate RCC cell lines with distinct histological subtypes (clear cell and papillary) and metastatic potential from non-tumorigenic renal cells. Hence, volatile organic compounds (VOCs) and volatile carbonyl compounds (VCCs) were extracted by headspace solid-phase microextraction (HS-SPME) and analyzed by gas chromatography–mass spectrometry (GC–MS). Multivariate and univariate analysis unveiled a panel of metabolites responsible for the separation between groups, mostly belonging to ketones, alcohols, alkanes and aldehydes classes. Some metabolites were found similarly altered for all RCC cell lines compared to non-tumorigenic cells, namely 2-ethylhexanol, tetradecane, formaldehyde, acetone (increased) and cyclohexanone and acetaldehyde (decreased). Furthermore, significantly altered levels of cyclohexanol, decanal, decane, dodecane and 4-methylbenzaldehyde were observed in all metastatic RCC cell lines when compared with the non-metastatic ones. Moreover, some alterations in the volatile composition were also observed between RCC histological subtypes. Overall, our results demonstrate the potential of volatile profiling for identification of noninvasive candidate biomarkers for early RCC diagnosis.

show abstract

The activity of class I, II, III and IV alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in renal cell carcinoma

Cited by 16 publications

References 27 publications

Distinct prognostic values of alcohol dehydrogenase mRNA expression in pancreatic adenocarcinoma

Distinct prognostic values of alcohol dehydrogenase mRNA expression in pancreatic adenocarcinoma

Prognostic value of alcohol dehydrogenase mRNA expression in gastric cancer

Volatilomics Reveals Potential Biomarkers for Identification of Renal Cell Carcinoma: An In Vitro Approach

Contact Info

Product

Resources

About