The activity of nonspecific esterases and glutathione-S-transferase in Locusta migratoria larvae infected with the fungus Metarhizium anisopliae (Ascomycota, Hypocreales)

Dubovskiy, Ivan M.; Slyamova, N. D.; Kryukov, V. Yu.; Yaroslavtseva, O. N.; Levchenko, M. V.; Belgibaeva, A. B.; Adilkhankyzy, A.; Глупов, В. В.

doi:10.1134/s0013873812010022

Cited by 39 publications

(34 citation statements)

References 18 publications

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“…Molting fluid is produced in response to pathogens in the ecdysis, between the layers of the cuticle and functions as immunity protection associated with melanisation. Bombyx mori 60,85 Nasonia species 86 Cellular immune Fungal infection of locus larvae enhances activity of GST activity Locusta migratoria 87 C1q-like venom protein GB43580 ↑ Venom but not allergen in wasp, C1q VP may be able to inhibit the phenoloxidase pathway, preventing encapsulation. This is a likely function of C1p VP in wasp venom.…”

Section: Resultsmentioning

confidence: 98%

Infections with the Sexually Transmitted Pathogen Nosema apis Trigger an Immune Response in the Seminal Fluid of Honey Bees (Apis mellifera)

et al. 2016

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Honey bee (Apis mellifera) males are highly susceptible to infections with the sexually transmitted fungal pathogen Nosema apis. However, they are able to suppress this parasite in the ejaculate using immune molecules in the seminal fluid. We predicted that males respond to infections by altering the seminal fluid proteome to minimize the risk to sexually transmit the parasite to the queen and her colony. We used iTRAQ isotopic labeling to compare seminal fluid proteins from infected and noninfected males and found that N. apis infections resulted in significant abundance changes in 111 of the 260 seminal fluid proteins quantitated. The largest group of proteins with significantly changed abundances consisted of 15 proteins with well-known immune-related functions, which included two significantly more abundant chitinases in the seminal fluid of infected males. Chitinases were previously hypothesized to be involved in honey bee antifungal activity against N. apis. Here we show that infection with N. apis triggers a highly specific immune response in the seminal fluid of honey bee males.

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Section: Resultsmentioning

confidence: 98%

Infections with the Sexually Transmitted Pathogen Nosema apis Trigger an Immune Response in the Seminal Fluid of Honey Bees (Apis mellifera)

et al. 2016

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“…However, our study showed that the activities of GST and β-EST were notably higher in larvae infected with blastospores when compared to conidial infections at 24 hr pi. This elevated activity is probably an attempt to inactivate toxic fungal metabolites or the pathogen [39]. Reduced EST and GST activity observed 36 h pi appears to be linked with EPF inhibition of the host defence system [40].…”

Section: Discussionmentioning

confidence: 99%

“…The host’s cells can protect themselves against these fluctuations by producing enzymatic and non-enzymatic antioxidants [36,37]. Enhanced activity of esterases has been observed in the fat body and hemolymph of Leptinotarsa decemlineata and Locusta migratoria infected with M. anisopliae [38,39] These enzymes play an important role in host protection against pathogens, where increased activity of these enzymes results in the degradation of toxic molecules produced during EPF infection [39,40]. …”

Section: Introductionmentioning

confidence: 99%

Highly specific host-pathogen interactions influence Metarhizium brunneum blastospore virulence against Culex quinquefasciatus larvae

et al. 2018

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Entomopathogenic fungi are potential biological control agents of mosquitoes. Our group observed that not all mosquitoes were equally susceptible to fungal infection and observed significant differences in virulence of different spore types. Conidiospores and blastospores were tested against Culex quinquefasciatus larvae. Blastospores are normally considered more virulent than conidia as they form germ tubes and penetrate the host integument more rapidly than conidia. However, when tested against Cx. quinquefasciatus, blastospores were less virulent than conidia. This host-fungus interaction was studied by optical, electron and atomic force microscopy (AFM). Furthermore, host immune responses and specific gene expression were investigated. Metarhizium brunneum (formerly M. anisopliae) ARSEF 4556 blastospores did not readily adhere to Culex larval integument and the main route of infection was through the gut. Adhesion forces between blastospores and Culex cuticle were significantly lower than for other insects. Larvae challenged with blastospores showed enhanced immune responses, with increased levels of phenoloxidase, glutathione-S-transferase, esterase, superoxide dismutase and lipid peroxidase activity. Interestingly, M. brunneum pathogenicity/stress-related genes were all down-regulated in blastospores exposed to Culex. Conversely, when conidia were exposed to Culex, the pathogenicity genes involved in adhesion or cuticle degradation were up-regulated. Delayed host mortality following blastospore infection of Culex was probably due to lower adhesion rates of blastospores to the cuticle and enhanced host immune responses deployed to counter infection. The results here show that subtle differences in host-pathogen interactions can be responsible for significant changes in virulence when comparing mosquito species, having important consequences for biological control strategies and the understanding of pathogenicity processes.

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“…These data suggest that the parasite may inhibit or decrease the relative activity of esterases. This might be related to the suppression of the insect's immune system against infection, a finding similar to what was observed in Locusta migratoria infected with Metarhizium anisopliae (Dubovskiy et al, 2012).…”

Section: Q3mentioning

confidence: 51%

Morpho-functional characterization and esterase patterns of the midgut of Tribolium castaneum Herbst, 1797 (Coleoptera: Tenebrionidae) parasitized by Gregarina cuneata (Apicomplexa: Eugregarinidae)

Gigliolli

Lapenta

Rúvolo-Takasusuki

et al. 2015

Micron

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The activity of nonspecific esterases and glutathione-S-transferase in Locusta migratoria larvae infected with the fungus Metarhizium anisopliae (Ascomycota, Hypocreales)

Cited by 39 publications

References 18 publications

Infections with the Sexually Transmitted Pathogen Nosema apis Trigger an Immune Response in the Seminal Fluid of Honey Bees (Apis mellifera)

Infections with the Sexually Transmitted Pathogen Nosema apis Trigger an Immune Response in the Seminal Fluid of Honey Bees (Apis mellifera)

Highly specific host-pathogen interactions influence Metarhizium brunneum blastospore virulence against Culex quinquefasciatus larvae

Morpho-functional characterization and esterase patterns of the midgut of Tribolium castaneum Herbst, 1797 (Coleoptera: Tenebrionidae) parasitized by Gregarina cuneata (Apicomplexa: Eugregarinidae)

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