The activity of paraoxonase type 1 (PON‐1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility

Abstract

SUMMARYParaoxonase 1 (PON-1) is a hydrolytic enzyme present in body fluids, capable of protecting cells against oxidative stress. The hypothesis was hereby to test that PON-1, present in seminal plasma (SP), acts protecting boar spermatozoa when showing a reasonable high activity in the ejaculate. SP-PON-1 activity differed (p < 0.001) among boars (from 0.10 to 0.29 IU/mL). Intra-boar variability was also observed (p < 0.05), but only in two of the 15 boars. SP-PON-1 activity differed among ejaculate portions, showing the spermatozoa-peak portion of spermatozoa-rich ejaculate fraction the highest levels (0.35 AE 0.03 IU/mL, ranging from 0.12 to 0.69) and the post-sperm ejaculate fraction the lowest levels (0.12 AE 0.01 IU/mL, ranging from 0.03 to 0.21). SP-PON-1 activity was positively correlated with the percentage of spermatozoa with rapid and progressive movement (p < 0.01) and negatively correlated with the generation of intracellular reactive oxygen species (p < 0.01) in semen samples after 72 h of liquid storage. SP-PON-1 activity was highest (p < 0.01) in boars with highest farrowing rates. In conclusion, SP-PON-1 activity differed among boars and ejaculate fractions/portions. SP-PON-1 activity was positively correlated with sperm quality and functionality of liquid-stored semen samples and it evidenced a positive association with in vivo fertility.

Will AI in pigs become more efficient?

“…This handling is now switching towards a growing interest to scrutinize SP-composition and evaluate its relevance for sperm functionality, even fertility. Recent findings support the complex composition of boar SP [50][51][52] and also demonstrate the relevance of some SP-components, such as Glutathione Peroxidase 5 [53] or Paraoxonase-I [54], for promoting sperm fertility of liquid stored semen IA-doses.…”

Profile and reproductive roles of seminal plasma melatonin of boar ejaculates used in artificial insemination programs

“…nents (Strzezek et al, 2005;Barranco et al, 2015Barranco et al, , 2016. Differences among boars could have been ascribed to the genetic origin that was more closely related to individuals than to breed (Thurston et al, 2001).…”

“…Therefore, this study will focus mainly on the investigation of the putative antioxidant properties of SP MLT, evaluating its impact on quality and sperm functionality. Some SP components with antioxidant capacities, such as glutathione peroxidase-5 and paraoxonase 1, have shown a direct relationship with in vivo fertility outcomes of boar semen AI doses (Novak et al, 2010;Barranco et al, 2015Barranco et al, , 2016. Accordingly, the study also aims to evaluate whether SP MLT is associated with the in vivo fertility of boar semen doses used in commercial AI programs.…”

Boar Differences In Artificial Insemination Outcomes: Can They Be Minimized?

“…; Barranco et al. ). These findings open for the quantification of certain components of SP and their use to identify the fertile potential of specific sires.…”

“…Moreover, SP peptide components, such as cytokines and chemokines, interact with the uterine epithelium inducing an active immune tolerance essential for fertilization and further embryo development (Rodriguez- ). Furthermore, some SP functional proteins with antioxidant properties, such as glutathione peroxidase 5 (GPX5) and paraoxonase-I, have proven to be related to boar fertility, allowing for discriminating boars per fertility levels, introducing them as potential fertility markers (Dyck et al 2011;Barranco et al 2015). These findings open for the quantification of certain components of SP and their use to identify the fertile potential of specific sires.…”