The activity of plant inner membrane anion channel (PIMAC) can be performed by a chloride channel (CLC) protein in mitochondria from seedlings of maize populations divergently selected for cold tolerance

Tampieri, Elisabetta; Baraldi, Elena; Carnevali, Francesco; Frascaroli, Elisabetta; Santis, Adelmo De

doi:10.1007/s10863-011-9386-z

Cited by 15 publications

(6 citation statements)

References 36 publications

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“…As previously observed for IMAC of mammalian mitochondria [97], evidence has been reported in seedlings of maize that a chloride channel protein, namely the ZmCLCc, can perform the PIMAC activity [42]. …”

Section: Pimac Carriers and Anion Transport In Energized And De-enermentioning

confidence: 66%

“…Consistently, evidence for a role of PIMAC in plant response to abiotic stress has been also reported in maize seedlings; the analysis of three maize populations differing in terms of cold tolerance highlighted a relationship between the PIMAC activity kinetics and the level of cold tolerance [38]; in addition, in the same plant material, the level of the ZmCLCc protein, responsible for the PIMAC activity in maize seedling, was found to be higher in cold stressed than in non-stressed growing conditions [42]. …”

Section: Pimac Carriers and Anion Transport In Energized And De-enermentioning

confidence: 99%

“…Recently, a major mitochondrial iron transporter, essential for plant growth and development, has been identified and characterized in rice [36,37]. With respect to anion conductance pathways, the occurrence of a plant inner membrane anion channel (PIMAC), catalyzing the electrophoretic uniport of different anions, has been functionally demonstrated in mitochondria from a few species [38–40]; a chloride channel activity has been described in potato [41], as well as chloride channel protein performing PIMAC activity has been identified in maize [42]. …”

Section: Introductionmentioning

confidence: 99%

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Transport Pathways—Proton Motive Force Interrelationship in Durum Wheat Mitochondria

Trono¹,

Laus

Soccio

et al. 2014

IJMS

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In durum wheat mitochondria (DWM) the ATP-inhibited plant mitochondrial potassium channel (PmitoKATP) and the plant uncoupling protein (PUCP) are able to strongly reduce the proton motive force (pmf) to control mitochondrial production of reactive oxygen species; under these conditions, mitochondrial carriers lack the driving force for transport and should be inactive. However, unexpectedly, DWM uncoupling by PmitoKATP neither impairs the exchange of ADP for ATP nor blocks the inward transport of Pi and succinate. This uptake may occur via the plant inner membrane anion channel (PIMAC), which is physiologically inhibited by membrane potential, but unlocks its activity in de-energized mitochondria. Probably, cooperation between PIMAC and carriers may accomplish metabolite movement across the inner membrane under both energized and de-energized conditions. PIMAC may also cooperate with PmitoKATP to transport ammonium salts in DWM. Interestingly, this finding may trouble classical interpretation of in vitro mitochondrial swelling; instead of free passage of ammonia through the inner membrane and proton symport with Pi, that trigger metabolite movements via carriers, transport of ammonium via PmitoKATP and that of the counteranion via PIMAC may occur. Here, we review properties, modulation and function of the above reported DWM channels and carriers to shed new light on the control that they exert on pmf and vice-versa.

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Section: Pimac Carriers and Anion Transport In Energized And De-enermentioning

confidence: 66%

Section: Pimac Carriers and Anion Transport In Energized And De-enermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transport Pathways—Proton Motive Force Interrelationship in Durum Wheat Mitochondria

Trono¹,

Laus

Soccio

et al. 2014

IJMS

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“…The maize CLCs , ZmCLC‐c , and ZmCLC‐d are induced by cold treatment, and the expression of ZmCLC‐c is reported higher in the cold‐tolerant population under control conditions (Tampieri et al ., 2011; Yang et al ., 2011). ZmCLC‐c is localized in the mitochondria where Cl − and I − fluxes were inhibited when treated with anti‐ZmCLC‐c antibody, suggesting that ZmCLC‐c is a plant inner membrane anion channel (PIMAC; Tampieri et al ., 2011). The induction of ZmCLC‐d expression, in addition to cold, has also been observed in drought, heat, NaCl, ABA, and H 2 O 2 treatments (Wang et al ., 2015).…”

Section: Clcs In Plantsmentioning

confidence: 99%

The chloride channels: Silently serving the plants

Subba

Tomar

Pareek

et al. 2020

Physiologia Plantarum

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Chloride channels (CLCs), member of anion transporting proteins, are present ubiquitously in all life forms. Diverging from its name, the CLC family includes both channel and exchanger (proton‐coupled) proteins; nevertheless, they share conserved structural organization. They are engaged in diverse indispensable functions such as acid and fluoride tolerance in prokaryotes to muscle stabilization, transepithelial transport, and neuronal development in mammals. Mutations in genes encoding CLCs lead to several physiological disorders in different organisms, including severe diseases in humans. Even in plants, loss of CLC protein function severely impairs various cellular processes critical for normal growth and development. These proteins sequester Cl− into the vacuole, thus, making them an attractive target for improving salinity tolerance in plants caused by high abundance of salts, primarily NaCl. Besides, some CLCs are involved in NO3− transport and storage function in plants, thus, influencing their nitrogen use efficiency. However, despite their high significance, not many studies have been carried out in plants. Here, we have attempted to concisely highlight the basic structure of CLC proteins and critical residues essential for their function and classification. We also present the diverse functions of CLCs in plants from their first cloning back in 1996 to the knowledge acquired as of now. We stress the need for carrying out more in‐depth studies on CLCs in plants, for they may have future applications towards crop improvement.

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“…Nonetheless, at least some hypotheses have been put forward on what proteins mediate the activities observed either by electrophysiology or by classical bioenergetics. For example, the CLCNt chloride channel of tobacco cells has been located to the IMM by biochemical tools (Lurin et al, 2000) and ClC from Zea mays seems to account for plant inner membrane anion channel (PIMAC) activity observed by classical bioenergetics (Tampieri et al, 2011). For the plant BK channel, based on biochemical indications, the authors proposed that an AtKC1-like channel might be involved (Koszela-Piotrowska et al, 2009).…”

Section: Mitochondrial Ion Channels Electrophysiology and Molecular Playersmentioning

confidence: 99%

Ion Channels in Plant Bioenergetic Organelles, Chloroplasts and Mitochondria: From Molecular Identification to Function

et al. 2016

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Recent technical advances in electrophysiological measurements, organelle-targeted fluorescence imaging, and organelle proteomics have pushed the research of ion transport a step forward in the case of the plant bioenergetic organelles, chloroplasts and mitochondria, leading to the molecular identification and functional characterization of several ion transport systems in recent years. Here we focus on channels that mediate relatively high-rate ion and water flux and summarize the current knowledge in this field, focusing on targeting mechanisms, proteomics, electrophysiology, and physiological function. In addition, since chloroplasts evolved from a cyanobacterial ancestor, we give an overview of the information available about cyanobacterial ion channels and discuss the evolutionary origin of chloroplast channels. The recent molecular identification of some of these ion channels allowed their physiological functions to be studied using genetically modified Arabidopsis plants and cyanobacteria. The view is emerging that alteration of chloroplast and mitochondrial ion homeostasis leads to organelle dysfunction, which in turn significantly affects the energy metabolism of the whole organism. Clear-cut identification of genes encoding for channels in these organelles, however, remains a major challenge in this rapidly developing field. Multiple strategies including bioinformatics, cell biology, electrophysiology, use of organelle-targeted ion-sensitive probes, genetics, and identification of signals eliciting specific ion fluxes across organelle membranes should provide a better understanding of the physiological role of organellar channels and their contribution to signaling pathways in plants in the future.

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The activity of plant inner membrane anion channel (PIMAC) can be performed by a chloride channel (CLC) protein in mitochondria from seedlings of maize populations divergently selected for cold tolerance

Cited by 15 publications

References 36 publications

Transport Pathways—Proton Motive Force Interrelationship in Durum Wheat Mitochondria

Transport Pathways—Proton Motive Force Interrelationship in Durum Wheat Mitochondria

The chloride channels: Silently serving the plants

Ion Channels in Plant Bioenergetic Organelles, Chloroplasts and Mitochondria: From Molecular Identification to Function

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