The Activity of Thymidine Phosphorylase in the Uterine Myomas and the Myometrium in Perimenopausal Women

Abstract

The activity of thymidine phosphorylase (dThdPase) in the myometrium and uterine myomas has been investigated in perimenopausal women. Differences in the activity of dThdPase have been found depending on the myoma type, menopause stage and the phase of the menstrual cycle in which the surgery was performed. The enzyme in the cytoplasmatic soluble fraction obtain ed at 50 000 x g was the most active in cellular leiomyomas of the follicular phase, the least in adenomyom as of the luteal phase of the menstrual cycle, whereas its activity in myometrium was always unchanged. Greater differences can be observed in the activity of dThdPase after a partial purification of the enzyme from myomas. It seems that the increase in dThdPase activity may point to its correlation with transient, premalignant tumor which may later transform into malignant forms.

6-Aminouracil: Geometries and spectra in the isolated state and in the solid state simulation. A comparison with 5-aminouracil

“…6-aminouracils find wide application as starting materials for the synthesis of many fused uracils of biological significance, for example, phenylazo- [16], pyrano-, pyrido-, pyrazolo-, pyrimido-and pyridazino-pyrimidines [17]. 6-amino substituted derivatives of thymine have been found to be the competitive inhibitors of human thymidine phosphorylase [18,19]. Other derivatives of 6-aminoU were also studied as possible muscle reluctant [20].…”

The cytosol activity of thymidine phosphorylase in endometrial cancer

“…We obtained similar observations for cytosol TP activity from ovarian [ 17 ] and breast carcinoma [ 16 ]. In benign and mixed uterine tumors [ 9 ] we also found a significantly higher mean TP activity (in the cytoplasmatic soluble fraction obtained at 50 000 × g and in partially purified fraction after DEAE-Sepharose) from only those women who were in the follicular phase of the cycle during surgery as compared to the control (myometrium), although the obtained TP activity values did not differ in the normal myometrium from women who were in luteal and in follicular phase of the menstrual cycle during surgery [ 9 ]. In our present data no statistically significant dependence has been found between the cytosol TP activities in normal endometrium in both phases of the menstrual cycle, either (data not published).…”

“…The increase of cytosol TP activity and a higher PD-ECGF/TP protein and PD-ECGF/TP gene expression as compared to the adjacent non-neoplastic tissues were reported in other gynecologic malignancies [9,16-18]. The data on PD-ECGF/TP protein expression and its correlation with microvessel density (MD) in endometrial cancer are controversial [19-21].…”

“…TP is identical with platelet-derived endothelial cell growth factor (PD-ECGF) which promotes angiogenesis [ 1 - 6 ]. TP activity, PD-ECGF/TP protein and PD-ECGF/TP gene expression are significantly increased in tumor tissues as compared to the adjacent non-neoplastic tissues in a variety of human carcinomas [ 7 - 9 ]. In certain tumors a high TP activity, as well as a high of PD-ECGF/TP protein and PD-ECGF/TP gene expression are associated with unfavorable prognosis [ 10 - 12 ].…”