The Acyl Homoserine Lactone Receptor, SdiA, of Escherichia coli and Salmonella enterica Serovar Typhimurium Does Not Respond to Indole

Sabag-Daigle, Anice; Soares, Jitesh A.; Smith, Jenée N.; Elmasry, Mohamed E.; Ahmer, Brian M. M.

doi:10.1128/aem.00046-12

Cited by 51 publications

(33 citation statements)

References 40 publications

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“…We observed that the type I triazole HLs with alkyl chains extending from the triazole ring ( i.e. , I-A – I-D ) displayed trends parallel to previously reported 3-oxo acyl-HLs; 23, 30 that is, triazole HLs with short alkyl chains were LasR antagonists, while triazole HLs with longer chains were LasR agonists (see below). As the alkyl chain increased in length from propyl ( I-A , 55%) to butyl ( I-B , 75%, IC 50 value = 17.6 μM), inhibitory activity increased from moderate to strong (Table 1).…”

Section: Resultssupporting

confidence: 86%

Synthesis and biological evaluation of triazole-containing N-acyl homoserine lactones as quorum sensing modulators

et al. 2013

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Many bacterial species are capable of assessing their local population densities through a cell-cell signaling mechanism termed quorum sensing (QS). This intercellular communication process is mediated by small molecule or peptide ligands and their cognate protein receptors. Numerous pathogens use QS to initiate virulence once they achieve a threshold cell number on a host. Consequently, approaches to intercept QS have attracted considerable attention as potential anti-infective therapies. Our interest in the development of small molecule tools to modulate QS pathways motivated us to evaluate triazole-containing analogs of natural N-acyl L-homoserine lactone (AHL) signals as non-native QS agonists and antagonists in Gram-negative bacteria. We synthesized 72 triazole derivatives of five broad structure types in high yields and purities using efficient Cu(I)-catalyzed azide-alkyne couplings. These compounds were evaluated for their ability to activate or inhibit two QS receptors from two prevalent pathogens – LasR from Pseudomonas aeruginosa and AbaR from Acinetobacter baumannii – using bacterial reporter strains. Several triazole derivatives were identified that were capable of strongly modulating the activity of LasR and AbaR. These compounds represent a new and synthetically accessible class of AHL analogs, and could find utility as chemical tools to study QS and its role in bacterial virulence.

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Section: Resultssupporting

confidence: 86%

Synthesis and biological evaluation of triazole-containing N-acyl homoserine lactones as quorum sensing modulators

et al. 2013

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“…Another gene potentially regulated by SdiA is srgE, which was acquired by horizontal transfer and has an unknown function (Sabag-Daigle et al, 2012). However, according to in silico nucleotide sequence analyses performed by the same authors, the SrgE protein may be involved in type III secretion systems (Sabag-Daigle et al, 2012).…”

Section: Quorum Sensing In Salmonella Enteritidis Pt4 578mentioning

confidence: 99%

“…However, according to in silico nucleotide sequence analyses performed by the same authors, the SrgE protein may be involved in type III secretion systems (Sabag-Daigle et al, 2012).…”

Section: Quorum Sensing In Salmonella Enteritidis Pt4 578mentioning

confidence: 99%

A new repertoire of informations about the quorum sensing system in Salmonella enterica serovar Enteritidis PT4

Campos-Galvão¹,

Leite²,

Ribon³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Salmonella spp are among the main causative agents of foodborne diseases. Some phenotypes associated with increased drug resistance and virulence are regulated by quorum sensing (QS). In the present study, the autoinducer (AI)-1-and -2-mediated QS mechanisms were characterized in Salmonella enterica serovar Enteritidis PT4 for the first time. Salmonella Enteritidis did not produce AI-1. Phylogenetic analysis of nucleotides encoding the SdiA protein, the response regulator of AI-1-mediated QS, and comparative alignment of its amino acids showed that the gene and protein are conserved within the same bacterial genus. Thus, bacteria of the same genus respond to the same AIs. However, this finding did not preclude the possibility that Salmonella Enteritidis might respond to AIs released from bacteria of a different genus, which might confer a competitive advantage to this pathogen. We found that the regulation of AI-2-mediated QS in Salmonella Enteritidis is similar to that in serovar Typhimurium. The elucidation of 4069 Quorum sensing in Salmonella ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 4068-4084 (2015) the AI-1-and AI-2-mediated QS mechanisms in Salmonella Enteritidis will contribute to the development of new control strategies for this pathogen by indicating new targets for antimicrobial drugs.

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“…However, another group determined that an indole associated decrease in biofilm formation in S. Typhimurium and E. coli was not dependent on SdiA. Instead, high indole concentrations were found to inhibit AHL detection by SdiA (Sabag-Daigle et al, 2012). …”

Section: Multidirectional Signaling In the Gut And Effect On S Typhimentioning

confidence: 99%

Salmonella Typhimurium and Multidirectional Communication in the Gut

et al. 2016

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The mammalian digestive tract is home to trillions of microbes, including bacteria, archaea, protozoa, fungi, and viruses. In monogastric mammals the stomach and small intestine harbor diverse bacterial populations but are typically less populated than the colon. The gut bacterial community (microbiota hereafter) varies widely among different host species and individuals within a species. It is influenced by season of the year, age of the host, stress and disease. Ideally, the host and microbiota benefit each other. The host provides nutrients to the microbiota and the microbiota assists the host with digestion and nutrient metabolism. The resident microbiota competes with pathogens for space and nutrients and, through this competition, protects the host in a phenomenon called colonization resistance. The microbiota participates in development of the host immune system, particularly regulation of autoimmunity and mucosal immune response. The microbiota also shapes gut–brain communication and host responses to stress; and, indeed, the microbiota is a newly recognized endocrine organ within mammalian hosts. Salmonella enterica serovar Typhimurium (S. Typhimurium hereafter) is a food-borne pathogen which adapts to and alters the gastrointestinal (GI) environment. In the GI tract, S. Typhimurium competes with the microbiota for nutrients and overcomes colonization resistance to establish infection. To do this, S. Typhimurium uses multiple defense mechanisms to resist environmental stressors, like the acidic pH of the stomach, and virulence mechanisms which allow it to invade the intestinal epithelium and disseminate throughout the host. To coordinate gene expression and disrupt signaling within the microbiota and between host and microbiota, S. Typhimurium employs its own chemical signaling and may regulate host hormone metabolism. This review will discuss the multidirectional interaction between S. Typhimurium, host and microbiota as well as mechanisms that allow S. Typhimurium to succeed in the gut.

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The Acyl Homoserine Lactone Receptor, SdiA, of Escherichia coli and Salmonella enterica Serovar Typhimurium Does Not Respond to Indole

Cited by 51 publications

References 40 publications

Synthesis and biological evaluation of triazole-containing N-acyl homoserine lactones as quorum sensing modulators

Synthesis and biological evaluation of triazole-containing N-acyl homoserine lactones as quorum sensing modulators

A new repertoire of informations about the quorum sensing system in Salmonella enterica serovar Enteritidis PT4

Salmonella Typhimurium and Multidirectional Communication in the Gut

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