The acylated (AG) to unacylated (UAG) ghrelin ratio in esterase inhibitor‐treated blood is higher than previously described

Abstract

AG and UAG levels measured in AEBSF-stabilized plasma indicate that the AG/UAG ratio is markedly higher than previously described and that UAG is a physiological component of the circulation. This highlights the importance of immediately stabilizing blood samples on collection for determination of both AG and UAG concentrations and provides a valuable tool for their measurement in physiological and interventional studies.

The Use of Oxytocin to Improve Feeding and Social Skills in Infants With Prader–Willi Syndrome

“…Measurements of acylated (AG) and unacylated ghrelin (UAG) and OXT in blood were performed as described. 15 …”

Des-acyl ghrelin attenuates pilocarpine-induced limbic seizures via the ghrelin receptor and not the orexin pathway

“…It could be that administration of ghrelin 30 min prior to pilocarpine administration, as opposed to 10 min as described by Biagini et al (2011) and Lucchi et al (2013), allowed ghrelin more time to reach the central ghrelin receptors to desensitize/internalize the receptors, resulting in an anticonvulsant effect in our case. Although it is known that acylated peptides cross the blood-brain barrier more quickly than their unacylated counterparts (Pardridge, 2012), it still remains a matter of debate whether the anticonvulsant effect following systemic ghrelin administration is in fact due to ghrelin acting on the central ghrelin receptors or due to the fact that ghrelin can be quickly converted to DAG (Delhanty et al, 2015;Satou et al, 2011). Whether DAG acts similarly to ghrelin or else activates different pathways when bound to the ghrelin receptor remains to be scrutinized.…”

Circulating acylghrelin levels are suppressed by insulin and increase in response to hypoglycemia in healthy adult volunteers

“…Furthermore, the analytical methods for measuring ghrelin concentrations have recently been updated (14). We therefore aimed to reassess the AG and UAG response to insulin-induced euglycemia and hypoglycemia using the most recent assay procedures (15).…”