The adaptation of lipid profile of human fibroblasts to alginate 2D films and 3D printed scaffolds

Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells

“…The goal of this work was the development of an ALG-based self-crosslinking bioink to be used in a 3D-printing extrusion-based system. Thus, fine control on the gelation speed is fundamental to obtain homogeneous formulations with such initial viscosities (8–40 kcP) to be printed by the custom low-temperature extrusion 3D-printing system developed for this work [ 8 , 29 , 30 , 31 ]. Increasing the viscosity of the initial solution (working at high alginate concentration or preparing blends of polymers) has been a strategy often used to obtain ALG bioinks suitable for 3D printing.…”

3D Printing Technologies in Biosensors Production: Recent Developments

“…As a consequence, these active materials have been used for different applications, such as regenerative medicine, drug delivery, treatment of infected wounds, and eco-friendly water purification systems [70][71][72][73]. Moreover, printable biomaterials can interact with cells by physical and chemical binding at different levels, from individual cells up to a single molecule as a function of time and system dimension [74]. In this scenario, the investigation of the interactions between liv- Regarding the employment of FDM technology for the manufacture of point-of-care (POC) devices, Pantazis et al [24] developed a 3D-printed bioreactor able to perform loop-mediated isothermal amplification (LAMP) on DNA collected from saliva samples to monitor the CYP2C19×2 mutation.…”

“…As a consequence, these active materials have been used for different applications, such as regenerative medicine, drug delivery, treatment of infected wounds, and eco-friendly water purification systems [70][71][72][73]. Moreover, printable biomaterials can interact with cells by physical and chemical binding at different levels, from individual cells up to a single molecule as a function of time and system dimension [74]. In this scenario, the investigation of the interactions between living cells and biomaterials could represent a valuable tool to better understand the mechanisms behind different molecular pathways of biology.…”

“…In this scenario, the investigation of the interactions between living cells and biomaterials could represent a valuable tool to better understand the mechanisms behind different molecular pathways of biology. Zanotti et al [74] investigated the adaptation of lipid the profile of human fibroblasts to alginate 2D films and 3D-printed scaffolds. The 3D alginate scaffold was constructed using a homemade 3D printer.…”

“…The behavior of human fibroblasts in contact with alginate hydrogels was demonstrated to be influenced by both architectures (2D and 3D). Intriguingly, 3D geometry can add an unknown physiologically relevant aspect compared with 2D [74]. Mainly in the biosensing field, but not only, the inkjet 3D printing techniques such as direct ink writing (DIW) and drop-on-demand (DOD), have also been widely employed for the fabrication of biosensors due to their advantages of contactless printing, reduction in waste, and rapid deposition [25,33,34,39].…”