The adhesive properties of coacervated recombinant hybrid mussel adhesive proteins

The Effect of Salt on the Complex Coacervation of Vinyl Polyelectrolytes

“…Consideration of these variables is critical in the design of self-assembled materials. For instance, electrostatic complexation between oppositely-charged polyelectrolytes has been used in drug and gene delivery [7,[14][15][16][17][18], thin film coatings [19][20][21], processed food [22], and underwater adhesives [23][24][25][26]. Clearly, the functionality of the material must be considered in the context of the operational environment.…”

“…As an example, the stability requirements for a material to be used in seawater (i.e., ~700 mM NaCl and pH ~7. 4-8.4) are significantly different from the requirements for stability under physiological conditions (i.e., ~150 mM KCl, ~20%-40% proteins by mass, and pH ~7.4 in the cytosol) [25,[27][28][29][30][31]. In order to achieve efficient materials design, it is necessary to develop a broader understanding of the role of individual parameters on materials self-assembly, rather than relying on application-specific investigations.…”

Linear viscoelasticity of complex coacervates

“…The self-assembly of these materials is driven by entropy, where the initial electrostatic attraction between oppositely-charged macro-ions results in the release of small, bound counter-ions and the restructuring of water molecules [1][2][3][4]. Complex coacervates have a long history of use in the food [5][6][7][8][9][10][11][12][13] and personal care [14,15] industries, and have found increasing utility as a platform for drug and gene delivery [1][2][3][4], as well as underwater adhesives [5][6][7][8][9][10][11][12][13][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62]. Coacervation has also recently been implicated in the formation of various biological assemblies [1,[14][15][16]55,…”

Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

“…15,[21][22][23] For in vitro DOPA modication, 2 g L À1 of puried original MAP was dissolved in 200 mM acetate buffer (pH 5.5) with 20 mM ascorbic acid and 20 mM boric acid. Mushroom tyrosinase (Sigma-Aldrich, St. Louis, MO) was then added to the solution to a nal concentration of 50 mg L…”

“…DOPA conversion of tyrosine residues was assessed by nitroblue tetrazolium (NBT) staining and amino acid composition analysis as followed in previous studies. 23,24 The mMAP was freeze dried for further procedures.…”