The Administration of Garlic Extract on Eimeria stiedai Oocysts and the Hematological Profile of the Coccidia Infected Rabbits

Abstract

This research aimed to examine the potential of garlic as the coccidiosis control in rabbits either in vitro or in vivo. During in vitro, observed variables were rabbits oocysts that were sporulated, unsporulated, and abnormal in incubation for 3 days with the addition of garlic extract. The treatments were doses of garlic extract administration (0%, 1%, 2%, 4%, and 8%) and sulfaquinoxalline as a standard anticoccidiosis. Meanwhile during in vivo, the variables observed were the hematological profile of the experimental rabbits naturally infected with coccidia. The doses of garlic extract was administered orally to the experimental rabbits infected with coccidia were 0 mg, 10 mg, 20 mg, 40 mg, and 80 mg/ rabbit. As a standard coccidiosis drugs, the combination of sulfadiazine and trimethoprim was used. The treatments were given for 6 days. The variables observed were the hematological profile of the coccidiosis rabbits, including the erythrocytes, hemoglobin, hematocrits, MCV, MCH, MCHC, and thrombocytes. The research employed a completely randomized design, with 5 repetitions. The data were further analyzed using the honestly significant difference test. The results showed that garlic extract administration significantly decreased (P<0.01) both the number of the sporulated and unsporulated oocysts (P<0.05), yet did not significantly influence the abnormal oocysts, but there was no significant difference within the entire hematological variables except in thrombocytes (P<0.05). Garlic extract administration decreased the excretion number of oocysts in the feces either in vitro or in vivo and influenced some hematological variables which provided a new propect for controlling coccidiosis naturally in rabbits.Keywords: coccidia, rabbits, garlic extract, oocyst, hematology ABSTRAK Penelitian ini bertujuan untuk menguji potensi bawang putih sebagai anti koksidiosis pada kelinci secara in vitro dan in vivo. Secara in vitro, peubah yang diamati adalah ookista kelinci yang bersporulasi, tidak bersporulasi, dan abnormal pada inkubasi selama 3 hari dengan penambahan ekstrak bawang putih. Perlakuan yang digunakan adalah 0%, 1%, 2%, 4%, dan 8% bawang putih, serta penggunaan sulfaquinoxalline sebagai obat standar koksidiosis. Pada penelitian in vivo, peubah yang diamati adalah profil hematologi kelinci yang terinfeksi koksidia secara alami. Ekstrak bawang putih diberikan secara per oral pada kelinci yang terinfeksi koksidia sebesar 0 mg, 10 mg, 20 mg, 40 mg, dan 80 mg/ekor, serta kombinasi sulfadiazin dan trimethoprim sebagai obat standar koksidiosis. Perlakuan diberikan selama 6 hari. Peubah yang diamati adalah profil darah kelinci koksidiosis yang meliputi sel darah merah (eritrosit), hemoglobin (Hb), hematokrit, MCV, MCH, MCHC, dan trombosit. Rancangan yang digunakan adalah rancangan acak lengkap (RAL), dengan pengulangan sebanyak 5 kali. Analisis data menggunakan analisis variansi dengan uji lanjut beda nyata jujur (BNJ). Hasil analisis varians menunjukkan bahwa pemberian ekstrak bawang putih berpengaruh sangat ...

Influence of allicin administration on reproductive efficiency, immunity and lipid peroxidation of rabbit does under high ambient temperature

“…Antioxidants from natural sources play a vital role in improving reproductive efficiency, immune response and health status of rabbit does (El‐Ratel et al, ). Allicin as a natural antioxidant was reported to have many biological activities for good health status, including anti‐microbial, anti‐inflammation, immunomodulatory and hypoglycaemic (Indrasanti, Indradji, Hastuti, Aprilliyani, & Rosyadi, ). According to the obtained results, oral administration of rabbit does with both allicin levels improved their reproductive efficiency (Table ).…”

Body Weight, Oocyte Elimination and Blood Profile of Rabbit After Challenge Test Using Eimeria stiedai

“…Blood profile represents hematologic status of animals, either plasma or blood plasma that essentially contributes to physiological process (Indrasanti et al, 2017). Blood profile of rabbits infected with various doses and subjected to challenge test with 10 3 Eimeria stiedai is presented in Table 3.…”

“…Two ml srynge was filled with 0,30 μl EDTA and injected through vena aucularis lateral (ears) to take 2 ml blood, then placed in a box filled with ice that had been prepared (Rukayah, 2008;;Indrasanti et al, 2017).…”

“…Oocyste fecal excretion is one of the indicators of coccidiosis infection. Blood tests are one of the parameters to determine the physiological conditions of livestock (Indrasanti et al, 2017). Rabbits infected with Eimeria spp.…”

Effect of turmeric and garlic supplementation to fermented Sauropus androgynus-bay leaves containing diet on fat deposition and broiler meat composition

“…Daneshyar et al (2011) reported that the inclusion of turmeric reduced thigh triglyceride, and saturated fatty acid content in the broiler. Garlic powder contains saponins, tannins, alkaloids, and flavonoids (Indrasanti et al, 2017). Kim and Kim (2011) reported that the inclusion of garlic reduced epididymal fat tissue in mice fed high-fat diet.…”