The Adrenal Cortex

Bertholf, Roger L.; Jialal, Ishwarlal; Winter, William E.

doi:10.1016/b978-1-4160-6164-9.00054-8

Cited by 6 publications

(5 citation statements)

References 319 publications

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“…Hence, without knowledge of the specific approach, it is difficult to speculate on individual assays. Nevertheless, it has been reported that manufacturers may displace cortisol from CBG by adjusting temperature, lowering pH or alternatively using agents such as salicylate or 8-anilino-1-napthalene sulfonic acid (ANS) (19 ). However, the efficiency of ANS is influenced by the concentration of binding proteins.…”

Section: Discussionmentioning

confidence: 99%

“…However, the efficiency of ANS is influenced by the concentration of binding proteins. Hence, the concentration of ANS required for healthy male and nonpregnant female samples may be inadequate for samples with increased CBG (19 ). Therefore, it is conceivable that immunoassays may rely on agents such as ANS that, when present in insufficient quantities, fail to completely free cortisol from CBG.…”

Section: Discussionmentioning

confidence: 99%

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Serum Cortisol: An Up-To-Date Assessment of Routine Assay Performance

Hawley¹,

Owen

Lockhart³

et al. 2016

Clinical Chemistry

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serum Cortisol: An Up-To-Date Assessment of Routine Assay Performance

Hawley¹,

Owen

Lockhart³

et al. 2016

Clinical Chemistry

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“…Little is known regarding the methods used by immunoassays to strip cortisol from CBG. The few published immunoassay methods that exist are from pre-commercial iterations, however, these mention using changes in temperature and pH or protein binding agents, such as 8-anilino-1-napthalene sulfonic acid (ANS) or salicylate 38,39,40 . Immunoassays relying on ANS may struggle with complete cortisol recovery in patients with raised CBG, since its efficiency is inversely proportional to binding globulin concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Immunoassays relying on ANS may struggle with complete cortisol recovery in patients with raised CBG, since its efficiency is inversely proportional to binding globulin concentration. 40 Further, these immunoassays must be stringent in their use of ANS, since it is known to inactivate antibodies. 41 This necessitates conservative use and makes under recovery hard to avoid.…”

Section: Discussionmentioning

confidence: 99%

Serum cortisol assay performance following the 1 mg overnight dexamethasone suppression test

et al. 2023

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Background: The 1 mg overnight dexamethasone suppression test (ONDST) is recommended for the differential diagnosis of Cushing’s syndrome and the investigation of adrenal incidentalomas. Despite documented variation in serum cortisol immunoassay performance, little has been published regarding its effect on the ONDST. Aims: Assess the performance of three immunoassay platforms (Roche Elecsys II, Abbott Alinity & Siemens Centaur) when compared to a liquid chromatography tandem mass spectrometry (LC-MS/MS) method. Methods: Samples (n=77) sent to the laboratory as part of an ONDST were retrieved prior to disposal, anonymised, and analysed on all platforms. Samples with factors impacting immunoassay analysis quality were excluded. Results were statistically compared to an LC-MS/MS method that previously demonstrated excellent comparability to a candidate reference method. Results: The Roche gen II showed a mean bias of -2.4 nmol/L and a Passing-Bablok fit of y = -0.9 + 0.97x. This was not affected by sex. The Abbott showed a mean bias -18.8 nmol/L, and a fit of y = -11.3 + 0.88x. This bias was -20.7 nmol/L in females vs -17.2 nmol/L in males. The Siemens had a mean bias of 2.3 nmol/L and a fit of y = 1.4 + 1.07x. This bias was 5.7 nmol/L in males vs -1.0 nmol/L in females. Conclusions: Clinicians should be aware of the method-dependent variation that exists within serum cortisol analysis during the ONDSTs. Roche and Siemens aligned more closely with LC-MS/MS while the Abbot may cause a reduction in ONDST sensitivity. This data supports assay-specific cut-offs for the ONDST.

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“…[20] Estimation of serum cortisol was done by solid-phase, competitive chemiluminescent enzyme immunoassay (IMMULITE® 1000 Immunoassay system) using kits manufactured by SIEMENS (USA). [21] Serum testosterone level was estimated on chemiluminescent immunoassay system (ADVIA Centaur CP) using kits manufactured by SIEMENS (USA).…”

Section: Introductionmentioning

confidence: 99%

Endogenous Cortisol and Testosterone Level in Central Serous Chorioretinopathy: A Case Control Study

Sachdeva¹

2017

WJPR

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Central serous chorioretinopathy (CSCR) is characterized by anexudative neurosensory layer detachment of retina. Male gender, type-A personality, emotional stress, pregnancy, infections, hormonal regulatory factors and several immunological reactions have all been implicated in causing CSCR. Although several hypotheses have tried to establish a link between endocrinal abnormalities and CSCR, still none is able to explain the true etiopathogenesis of CSCR. This study was designed to estimate serum cortisol and testosterone levels in patients of CSCR and study their potential role in etiopathogenesis of the disease. In this study, 25 patients of CSCR satisfying the inclusion and exclusion criteria were enrolled as cases and 25 age and sex matched patients with an acute unilateral rhegmatogenous retinal detachment (RD) were enrolled as controls. Levels of serum cortisol and testosterone were estimated in both groups. Serum cortisol measurement was done twice, due to diurnal variation in its levels. Data analysis was done by Pearson's correlation analysis and independent student t-test. The 8:00 AM mean serum cortisol value in the cases (20.21 ± 4.86 μg/dl) was significantly (p = 0.046) higher than controls (17.74 ± 3.53 μg/dl). Although the 11:00 PM mean serum cortisol value of cases (8.13 ± 3.52 μg/dl)

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The Adrenal Cortex

Cited by 6 publications

References 319 publications

Serum Cortisol: An Up-To-Date Assessment of Routine Assay Performance

Serum Cortisol: An Up-To-Date Assessment of Routine Assay Performance

Serum cortisol assay performance following the 1 mg overnight dexamethasone suppression test

Endogenous Cortisol and Testosterone Level in Central Serous Chorioretinopathy: A Case Control Study

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