The adsorption kinetics of cephalosporin-c on non-ionic polymeric macropore Amberlite XAD-16 resin

Yang, Se-Il; Pyle, D.L.

doi:10.1002/(sici)1097-4660(199903)74:3<216::aid-jctb44>3.0.co;2-j

Cited by 18 publications

(15 citation statements)

References 8 publications

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“…The fresh resin was determined to have an epothilone/resin loading capacity of approximately 80 mg/g. This loading capacity is similar to the 125-mg/g saturation loading at the same resin concentration reported by Yang and Pyle [23] in their study of cephalosporin-C binding to fresh XAD-16 resin. The M. xanthus spent resin has a saturation capacity which is approximately 20% lower than the fresh resin (63 mg/g), while the spent resin from the S. cellulosum cultivation has a saturation capacity approximately 50% lower than the fresh resin (41 mg/g).…”

Section: Fouling Of Product Adsorption Resin During a Microbial Cultimentioning

confidence: 99%

“…There is still excess epothilone binding capacity on both types of spent resins, but the reduction in their binding capacities will result in a decrease in the capture rate of further product. It is also possible that a number of the pores in these spent resin beads have become plugged with cells or other matter, decreasing the pore diffusivity rate of the resin and its contribution to the overall binding rate of the epothilone [23]. This decreased capture rate could then result in an increase in the unbound concentration of epothilone D, possibly leading to product repression and enhanced product degradation rates.…”

Section: Fouling Of Product Adsorption Resin During a Microbial Cultimentioning

confidence: 99%

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Characterization of product capture resin during microbial cultivations

Frykman¹,

Tsuruta²,

Galazzo³

et al. 2006

J IND MICROBIOL BIOTECHNOL

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Various bioactive small molecules produced by microbial cultivation are degraded in the culture broth or may repress the formation of additional product. The inclusion of hydrophobic adsorber resin beads to capture these products in situ and remove them from the culture broth can reduce or prevent this degradation and repression. These product capture beads are often subjected to a dynamic and stressful microenvironment for a long cultivation time, affecting their physical structure and performance. Impact and collision forces can result in the fracturing of these beads into smaller pieces, which are difficult to recover at the end of a cultivation run. Various contaminating compounds may also bind in a non-specific manner to these beads, reducing the binding capacity of the resin for the product of interest (fouling). This study characterizes resin bead binding capacity (to monitor bead fouling), and resin bead volume distributions (to monitor bead fracture) for an XAD-16 adsorber resin used to capture epothilone produced during myxobacterial cultivations. Resin fouling was found to reduce the product binding capacity of the adsorber resin by 25-50%. Additionally, the degree of resin bead fracture was found to be dependent on the cultivation length and the impeller rotation rate. Microbial cultivations and harvesting processes should be designed in such a way to minimize bead fragmentation and fouling during cultivation to maximize the amount of resin and associated product harvested at the end of a run.

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Section: Fouling Of Product Adsorption Resin During a Microbial Cultimentioning

confidence: 99%

Section: Fouling Of Product Adsorption Resin During a Microbial Cultimentioning

confidence: 99%

Characterization of product capture resin during microbial cultivations

Frykman¹,

Tsuruta²,

Galazzo³

et al. 2006

J IND MICROBIOL BIOTECHNOL

View full text Add to dashboard Cite

show abstract

“…They fitted the effective Sherwood number employed by the agitation method against the experiments. Yang et al 12 identified the significance of pore and surface diffusions and estimated the values by the nonlinear regression and "the half-time" methods. However, the diffusion coefficient needs to be further simplified in engineering application.…”

Section: ■ Introductionmentioning

confidence: 99%

Adsorption of Cefocelis Hydrochloride on Macroporous Resin: Kinetics, Equilibrium, and Thermodynamic Studies

Xue

Yingting

et al. 2016

J. Chem. Eng. Data

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The adsorptions of Cefocelis hydrochloride in aqueous solution on macroporous resin (HP-20) were studied. On the basis of static experiments with HP-20 resin, the adsorption kinetics, isotherms, and thermodynamics of Cefocelis hydrochloride in aqueous solution on macroporous resin (HP-20) were investigated. Adsorption equilibrium data were correlated with the Langmuir and Freundlich equations. Adsorption data of kinetic were modeled using the Crank equation and fitting for the diffusion coefficient (D e ), and the value of the liquid film mass transfer coefficient (k f ) was estimated from Carberry equation. Dynamic adsorption was performed on HP-20 resin packed in a glass column to obtain optimal parameters. The initial concentration, bed height, and residence time were considered to determine the dynamic test for the breakthrough curve. The values of thermodynamic parameters including the isosteric adsorption enthalpy (ΔH), free energy (ΔG), entropy (ΔS), and adsorption activation energy (E a ) demonstrated the process of adsorption was spontaneous and exothermic. The adsorption process was controlled by a physical mechanism.

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“…The intraparticle mass-transfer resistance is often dominant compared with external processes, especially when the particle size is relatively large (e.g. 0.3-1.0 mm) (Yang and Pyle 1999;Ma et al 1996). An understanding of the significance of diffusion processes and accurate estimates of the diffusivities within the adsorbent particles are both fundamental for modelling adsorption processes.…”

Section: Diffusion-controlled Kinetic Modelsmentioning

confidence: 99%

Adsorption Kinetics for the Removal of Nitrite Ions from Aqueous Solutions by an Ion-Exchange Resin

Kobya

Demirbaş

Yeşilot

et al. 2006

Adsorption Science & Technology

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ABSTRACT:The adsorption kinetics of nitrite ions in aqueous solutions onto an anion-exchange resin A-250 was explored in a well-stirred tank. The capacity of the ion-exchange resin for the removal of nitrite ions from aqueous solution was investigated under different conditions, viz. initial concentration (10-50 mg/l), pH (2-8), particle size (565-850 µm) and resin dosage (2-8 g/l). A number of kinetic models such as those of external and intraparticle diffusion were applied to the results from this study in order to identify the adsorption mechanism.The results correlated well with the intraparticle diffusion model. The experimental parameters had an effect on the effective pore and surface diffusivities. Adsorption of nitrite ions onto the resin showed that the latter could be used as an efficient adsorbent material for the removal of nitrite ions from aqueous solutions.

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The adsorption kinetics of cephalosporin-c on non-ionic polymeric macropore Amberlite XAD-16 resin

Cited by 18 publications

References 8 publications

Characterization of product capture resin during microbial cultivations

Characterization of product capture resin during microbial cultivations

Adsorption of Cefocelis Hydrochloride on Macroporous Resin: Kinetics, Equilibrium, and Thermodynamic Studies

Adsorption Kinetics for the Removal of Nitrite Ions from Aqueous Solutions by an Ion-Exchange Resin

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