The adsorption of globular proteins onto a fluorinated PDMS surface

Wang, Dan; Douma, Michelle; Swift, Brenna E; Oleschuk, Richard D.; Horton, J. Hugh

doi:10.1016/j.jcis.2008.11.010

Cited by 27 publications

(18 citation statements)

References 48 publications

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“…86 Several models of protein absorption on surfaces identify two main steps in the process. The first step could involve the arrival of the protein at the interface, through a diffusion process following the Brownian law of motion, and its further collision with the solid surface.…”

Section: Dovepressmentioning

confidence: 99%

Overview of the main methods used to combine proteins with nanosystems: absorption, bioconjugation, and encapsulation

Marco¹

2009

IJN

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Section: Dovepressmentioning

confidence: 99%

Overview of the main methods used to combine proteins with nanosystems: absorption, bioconjugation, and encapsulation

Marco¹

2009

IJN

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“…21,22 In addition, Wang, et al demonstrated solution phase grafting of perfluoronated alkoxysilane on PDMS microchannels prior to device bonding, but found that the silanization step produced weaker, reversible PDMS sealing. 23,24 To overcome this drawback, others have demonstrated solution phase silanization after PDMS bonding (where the bond strength of plasma-exposed PDMS is irreversible) through a similar two step process with octadecyltrichlorosilane (ODTS) 25,26 in PDMS devices used to produce aqueous plugs in flowing oil within the channels. The combination of methods and materials described herein specifically expands the use of PDMS materials for obtaining a hydrophobic surface suitable for stabilizing water-in-fluorocarbon oil assemblies in zero-flow applications.…”

Section: Fabrication and Silanization Of Microstructured Substratesmentioning

confidence: 99%

Physical encapsulation and controlled assembly of lipid bilayers within flexible substrates

Sarles

Leo

2010

Active and Passive Smart Structures and Integrated Systems 2010

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Biomolecular networks formed from droplet interface bilayers (DIB) use principles of phase separation and molecular self-assembly to create a new type of functional material. The original DIB embodiment consists of lipid-encased aqueous droplets surrounding by a large volume of oil contained in a shallow well. However, recent results have shown that, by reducing the amount of oil that separates the droplets from the supporting substrate, physically-encapsulated DIBs display increased durability and portability. In this paper we extend the concept of encapsulated biomolecular networks to one in which phase separation and molecular self-assembly occur entirely within internally-structured reservoirs of a solid material. Flexible substrates with 200μm wideby-200μm deep internal microchannels for holding the aqueous and oil phases are fabricated from Sylgard 184 polydimethylsiloxane (PDMS) using soft-lithography microfabrication techniques. Narrowed apertures along the microchannels enable the use of the regulated attachment method (RAM) to subdivide and reattach lipid-encased aqueous volumes contained within the material with an applied external force. The use of perfluorodecalin, a fluorocarbon oil, instead of hexadecane eliminates absorption of the oil phase into the PDMS bulk while a silanization surface treatment of the internal channel walls maximizes wetting by the oil phase to retain a thin layer of oil within the channels to provide a fluid oil/water interface around the aqueous volumes. High-quality 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPHPC) lipid bilayers formed within the prototype substrates have electrical resistance between 1 − 100GΩ, enabling the measurement of single and few-channel recordings of alpha-hemolysin (αHL) and alamethicin proteins incorporated into the bilayers.

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“…For instance, it is easy to fabricate, optically transparent, biocompatible, and inexpensive. Despite these advantages, applications of microfluidic devices fabricated in PDMS have not been applicable to bioanalysis for the use of small molecules which are absorbed and dispersed into PDMS due to its porous property (Toepke and Beebe 2006;Ou et al 2009;Wang et al 2009). The absorption property of PDMS makes it difficult to conduct intensity-based bioanalysis using hydrophobic fluorescent dyes ranging from Nile red (Toepke and Beebe 2006) to Rhodamine B (RhB) (Glawdel et al 2009) due to the increased background noise signals of the targeted molecules (Toepke and Beebe 2006).…”

Section: Introductionmentioning

confidence: 99%

Stable nonpolar solvent droplet generation using a poly(dimethylsiloxane) microfluidic channel coated with poly-p-xylylene for a nanoparticle growth

Lim

Moon

2015

Biomed Microdevices

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Applications of microfluidic devices fabricated in poly(dimethylsiloxane) (PDMS) have been limited to water-based analysis rather than nonpolar solvent based chemistry due to a PDMS swelling problem that occurs by the absorption of the solvents. The absorption and swelling causes PDMS channel deformation in shape, and changes the cross sectional area making it difficult to control the flow rate and concentrations of solution in PDMS microfluidic channels. We propose that poly-p-xylylene polymers (parylenes) are chemical vapors deposited on the surfaces of PDMS channels that alleviate the effect of solvents on the absorption and swelling. The parylene coated surface sustains 3 h with a small volumetric change (less than 22 % of PDMS swelling ratio). By generating an air-nonpolar solvent interface based on droplets in PDMS channel, we confirmed poly-p-xylylene coated PDMS microfluidic channels have the potential to be applicable to nanocrystal growth using nonpolar solvents.

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The adsorption of globular proteins onto a fluorinated PDMS surface

Cited by 27 publications

References 48 publications

Overview of the main methods used to combine proteins with nanosystems: absorption, bioconjugation, and encapsulation

Overview of the main methods used to combine proteins with nanosystems: absorption, bioconjugation, and encapsulation

Physical encapsulation and controlled assembly of lipid bilayers within flexible substrates

Stable nonpolar solvent droplet generation using a poly(dimethylsiloxane) microfluidic channel coated with poly-p-xylylene for a nanoparticle growth

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