“…Biodiesel production has three generations: (a) in the first generation the raw material is food, especially conventional sources of oil such as rapeseed, rapeseed, soy, castor beans, coconut, palm, sunflower and corn; (b) in the second generation, the raw material is inedible plants, for example: cotton seed, linseed, rubber seed and tobacco; and, (c) in the third generation, the raw material is used cooking oil, residual animal fats and from lipids produced by microalgae -the focus of this research (TAYLOR et al, 2013;PIKULA et al, 2020;MIZIK;GYARMATI, 2021).…”