The AE765, AK423, AK692, AO233 and AO234 antibodies recognize human actin by immunofluorescence in HEK cells

Abstract

The AE765, AK692, AO233 and AO234 antibodies recognize human actin in PAF-fixed HEK cells by immunofluorescence. Phalloidin, which recognizes polymerized actin, is more adequate to label actin filamentous structures. The AK423 antibody recognizes human actin in methanol-fixed HEK cells, but in these conditions the actin network is poorly preserved.

The AW288 and AX067 antibodies recognize human actin by immunofluorescence in HEK cells

“…1B, pinheads). As discussed previously (Marchetti, 2022a), this cytosolic staining might represent nonpolymerized actin.…”