The Affinity for Methane and Methanol of Mixed Cultures Grown on Methane in Continuous Culture

Wilkinson, T. G.; Harrison, David E.

doi:10.1111/j.1365-2672.1973.tb04107.x

Cited by 32 publications

(13 citation statements)

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“…It has been suggested that the increased stability of mixed cultures of methane-utilizing bacteria and heterotrophs is due to the utilization of inhibitory intermediate oxidation products of methane by the heterotrophic bacteria (Pirt, 1972;Wilkinson & Harrison, 1973). In our study methanol and formate were not detected in culture supernatants of the methaneutilizing bacterium Methylococcus sp.…”

Section: Discussionmentioning

confidence: 53%

“…Samples with low activity were incubated for 2 h. Measurement of& values. The effect of various concentrations of methane and methanol on the respiration rate of samples taken from chemostat cultures were determined using an oxygen electrode cell (Harrison, 1973) and K, values for methane and methanol were calculated.…”

Section: Lipolytic Activity Was Detected Using Oxoid Tributyrin Agar mentioning

confidence: 99%

“…The instability of pure cultures growing on methane may be due to the accumulation of inhibitory intermediate oxidation products of methane which are removed by the heterotrophic bacteria present in mixed cultures (Wilkinson & Harrison, 1973). Indeed, growth of a methane-utilizing pseudomonad in pure culture is inhibited by the accumulation of methanol (Wilkinson & Harrison, 1973). In a stable mixed culture system, the methanol produced by the methane-utilizing pseudomonad was utilized by a Hyphomicrobium sp.…”

Section: Introductionmentioning

confidence: 99%

“…Mixed cultures growing on methane as the sole source of carbon and energy grow better and exhibit greater stability than pure cultures (Sheehan & Johnson, 1971 ;Harrison et al, 1975). The instability of pure cultures growing on methane may be due to the accumulation of inhibitory intermediate oxidation products of methane which are removed by the heterotrophic bacteria present in mixed cultures (Wilkinson & Harrison, 1973). Indeed, growth of a methane-utilizing pseudomonad in pure culture is inhibited by the accumulation of methanol (Wilkinson & Harrison, 1973).…”

Section: Introductionmentioning

confidence: 99%

“…and a FZavobacterium sp. was to remove complex biological growth or cell lysis products (Wilkinson & Harrison, 1973 ;Wilkinson, Topiwala & Hamer, 1974). This paper reports a study of the interactions between component species of a different mixed culture grown on methane as the sole source of carbon and energy.…”

Section: Introductionmentioning

confidence: 99%

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Interactions in a Methane-utilizing Mixed Bacterial Culture in a Chemostat

Linton

Buckee

1977

Journal of General Microbiology

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219The interactions between the component species of a mixed bacterial culture grown on methane were investigated. The culture contained the methane-utilizing bacterium Methylococcus sp. and four heterotrophic bacteria which were unable to grow on methane but utilized products released by lysis of the Methylococcus sp. Products of methane oxidation such as methanol, formaldehyde and formate did not constitute a significant amount of the organic carbon found in the culture supernatant of the pure methane-utilizing bacterium grown in chemostat culture. The organic carbon was accounted for mainly as protein and nucleic acid and originated from a growth-dependent lysis of the methane-utilizing bacterium. In the mixed culture the level of the organic carbon was reduced and it is suggested that extracellular enzymes produced by the heterotrophic bacteria play a role in the degradation and utilization of these compounds. The major heterotrophic bacterium Pseudomonas sp. NCIBI 13 LO produced an extracellular neutral protease that degraded the proteins present in the culture supernatant of the methane-utilizing bacterium.

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Section: Discussionmentioning

confidence: 53%

Section: Lipolytic Activity Was Detected Using Oxoid Tributyrin Agar mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interactions in a Methane-utilizing Mixed Bacterial Culture in a Chemostat

Linton

Buckee

1977

Journal of General Microbiology

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Numerical modeling and uncertainties in rate coefficients for methane utilization and TCE cometabolism by a methane-oxidizing mixed culture

Smith

Kitanidis

McCarty

1997

Biotechnol. Bioeng.

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The rates of methane utilization and trichloroethylene (TCE) cometabolism by a methanotrophic mixed culture were characterized in batch and pseudo‐steady‐state studies. Procedures for determination of the rate coefficients and their uncertainties by fitting a numerical model to experimental data are described. The model consisted of a system of differential equations for the rates of Monod kinetics, cell growth on methane and inactivation due to TCE transformation product toxicity, gas/liquid mass transfer of methane and TCE, and the rate of passive losses of TCE. The maximum specific rate of methane utilization (k CH 4) was determined by fitting the numerical model to batch experimental data, with the initial concentration of active methane‐oxidizing cells (X0a) also used as a model fitting parameter. The best estimate of k CH 4 was 2.2 g CH4/g cells‐d with excess copper available, with a single‐parameter 95% confidence interval of 2.0–2.4 mg/mg‐d. The joint 95% confidence region for k CH 4 and X0a is presented graphically. The half‐velocity coefficient (K italicS,CH 4) was 0.07 mg CH4/L with excess copper available and 0.47 mg CH4/L under copper limitation, with 95% confidence intervals of 0.02–0.11 and 0.35–0.59 mg/L, respectively. Unique values of the TCE rate coefficients kTCE and KS,TCE could not be determined because they were found to be highly correlated in the model fitting analysis. However, the ratio kTCE/KS,TCE and the TCE transformation capacity (TC) were well defined, with values of 0.35 L/mg‐day and 0.21 g TCE/g active cells, respectively, for cells transforming TCE in the absence of methane or supplemental formate. The single‐parameter 95% confidence intervals for kTCE/KS,TCE and TC were 0.27–0.43 L/mg‐d and 0.18–0.24 g TCE/g active cells, respectively. The joint 95% confidence regions for kTCE/KS,TCE and TC are presented graphically. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 320–331, 1997.

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Interactions in a mixed bacterial population growing on methane in continuous culture

Wilkinson

Topiwala

Hamer

1974

Biotech & Bioengineering

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143

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SummaryThe growth of a mixed methane-utilizing culture in a continuous flow fermenter has been studied under both methane and oxygen limitation. Small additions of methanol have been shown to inhibit the methane-utilizing moiety in the culture and it has been shown that the Hyphomicrobium sp. in the mixed culture removes any inhibitory methanol. The interaction between the methane-utilizing Pseudomonas sp., and the Hyphomicrobium sp. has been explained and a model of the continuous mixed culture under oxygen limitation has been formulated. Qualitative predictions of transient phenomena by the model have been verified experimentally.

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The Affinity for Methane and Methanol of Mixed Cultures Grown on Methane in Continuous Culture

Cited by 32 publications

References 6 publications

Interactions in a Methane-utilizing Mixed Bacterial Culture in a Chemostat

Interactions in a Methane-utilizing Mixed Bacterial Culture in a Chemostat

Numerical modeling and uncertainties in rate coefficients for methane utilization and TCE cometabolism by a methane-oxidizing mixed culture

Interactions in a mixed bacterial population growing on methane in continuous culture

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