The Aflibercept-Induced MicroRNA Profile in the Vitreous of Proliferative Diabetic Retinopathy Patients Detected by Next-Generation Sequencing

Guo, Ju; Zhou, Pengyi; Liu, Zhenhui; Dai, Fangfang; Pan, Meng; An, Guangqi; Han, Jinfeng; Du, Liping; Jin, Xuemin

doi:10.3389/fphar.2021.781276

Cited by 5 publications

(4 citation statements)

References 48 publications

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“…Evidence has accumulated that ncRNAs, including miRNAs, long ncRNAs (lncRNAs), and circRNAs, have an essential role in the occurrence and development of DR ( 19 ). Several miRNAs, such as hsa-miR-3184-3p, hsa-miR-24-3p, and hsa-miR-197-3p, are overexpressed in the vitreous humor of PDR patients, and intravitreal injection of anti-VEGF drugs inhibits the increase of these miRNAs ( 20 ). Despite the direct regulatory actions of target genes on mRNA expression levels, miRNAs also serve as targets of the competitive endogenous RNA (ceRNA) network, which is constructed using lncRNAs or circRNAs ( 21 ).…”

Section: Discussionmentioning

confidence: 99%

Altered Expressions of Transfer RNA-Derived Small RNAs and microRNAs in the Vitreous Humor of Proliferative Diabetic Retinopathy

et al. 2022

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PurposeWe sought to reveal the expression profiles of transfer RNA-derived small RNAs (tsRNAs) and microRNAs (miRNAs) in the vitreous humor of patients with proliferative diabetic retinopathy (PDR).MethodsVitreous humor samples were obtained from PDR patients and a control group for this study. Sequencing of small RNAs was conducted to assess the expression profiles of tsRNAs and miRNAs in both groups, which was followed by validation using reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). Bioinformatics analyses were conducted to predict the target genes and their potential biological functions and signaling pathways.ResultsA total of 37 tsRNAs and 70 miRNAs with significant differences were screened out from the vitreous humor samples of PDR patients compared to controls. Following validation by RT-qPCR, the target genes of the validated tsRNAs and miRNAs were predicted, and Gene Ontology analysis indicated that the target genes of the tsRNAs were most enriched in the cellular macromolecule metabolic process, cytoplasm, and ion-binding, while those of the miRNAs were most abundant in the regulation of major metabolic process, cytoplasm, and protein-binding. In addition, Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the target genes of said tsRNAs and miRNAs were most enriched in the adenosine monophosphate-activated protein kinase signaling pathway and Th17 cell differentiation, respectively.ConclusionsThe present study identified altered tsRNAs and miRNAs in vitreous humor samples of PDR patients, which may play important roles in the pathogenesis of PDR and could be considered potential therapeutic targets in the treatment of PDR.

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Section: Discussionmentioning

confidence: 99%

Altered Expressions of Transfer RNA-Derived Small RNAs and microRNAs in the Vitreous Humor of Proliferative Diabetic Retinopathy

et al. 2022

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“… 40 Overexpression of hsa-miR-185-5p occurs in the vitreous of proliferative diabetic retinopathy patients, 41 in the pericardiac adipose tissue of patients with coronary artery disease, 42 and significantly enhances endothelial cell angiogenesis. 43 hsa-miR-197-3p is highly expressed in proliferative diabetic retinopathy patients, 44 , 45 is involved in the development of colorectal cancer 46 and non-small cell lung cancer, 47 and may act as a biomarker for follicular thyroid cancer. 48 Further, hsa-miR-485-5p is associated with lupus nephritis, 49 pulmonary tuberculosis, 50 and cancers such as lung cancer, 51 hepatocellular carcinoma, 52 oral tongue squamous cell carcinoma, 53 and glioblastoma.…”

Section: Discussionmentioning

confidence: 99%

The Network of miRNA–mRNA Interactions in Circulating T Cells of Patients Following Major Trauma – A Pilot Study

Rau

Kuo

Lin

et al. 2022

JIR

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Purpose Following major trauma, genes involved in adaptive immunity are downregulated, which accompanies the upregulation of genes involved in systemic inflammatory responses. This study investigated microRNA (miRNA)-mRNA interactome dysregulation in circulating T cells of patients with major trauma. Patients and Methods This study included adult trauma patients who had an injury severity score ≥16 and required ventilator support for more than 48 h in the intensive care unit. Next-generation sequencing was used to profile the miRNAs and mRNAs expressed in CD3+ T cells isolated from patient blood samples collected during the injury and recovery stages. Results In the 26 studied patients, 9 miRNAs (hsa-miR-16-2-3p, hsa-miR-16-5p, hsa-miR-185-5p, hsa-miR-192-5p, hsa-miR-197-3p, hsa-miR-23a-3p, hsa-miR-26b-5p, hsa-miR-223-3p, and hsa-miR-485-5p) were significantly upregulated, while 58 mRNAs were significantly downregulated in T cells following major trauma. A network consisting of 8 miRNAs and 22 mRNAs interactions was revealed by miRWalk, with three miRNAs (hsa-miR-185-5p, hsa-miR-197-3p, and hsa-miR-485-5p) acting as hub genes that regulate the network. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis suggested that “chemokine signaling pathway” was the predominant pathway. Conclusion The study revealed a miRNA-mRNA interactome consisting of 8 miRNAs and 22 mRNAs that are predominantly involved in chemokine signaling in circulating T cells of patients following major trauma.

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“…Plasma results among T2DM patients gave an insight into lower levels of miRNA-29b in the DR group and miRNA-21 as biomarkers that were significantly associated with PDR. Other parameters that were increased in T2DM patients with DR were miRNA-93 via SIRT1 and miRNA-21, as well as miRNA-152 [126,127]. On the contrary, miRNA-15a, miRNA-20b, miRNA-21, miRNA-24, miRNA-320, miRNA-486, and miRNA-150, miRNA-126, miRNA-191, miRNA-197 are downregulated in that group of patients' plasma samples [128].…”

Section: Micrornasmentioning

confidence: 92%

Advances and Perspectives in Relation to the Molecular Basis of Diabetic Retinopathy—A Review

Błaszkiewicz,

Walulik,

Florek

et al. 2023

Biomedicines

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Diabetes mellitus (DM) is a growing problem nowadays, and diabetic retinopathy (DR) is its predominant complication. Currently, DR diagnosis primarily relies on fundoscopic examination; however, novel biomarkers may facilitate that process and make it widely available. In this current review, we delve into the intricate roles of various factors and mechanisms in DR development, progression, prediction, and their association with therapeutic approaches linked to the underlying pathogenic pathways. Specifically, we focus on advanced glycation end products, vascular endothelial growth factor (VEGF), asymmetric dimethylarginine, endothelin-1, and the epigenetic regulation mediated by microRNAs (miRNAs) in the context of DR.

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The Aflibercept-Induced MicroRNA Profile in the Vitreous of Proliferative Diabetic Retinopathy Patients Detected by Next-Generation Sequencing

Cited by 5 publications

References 48 publications

Altered Expressions of Transfer RNA-Derived Small RNAs and microRNAs in the Vitreous Humor of Proliferative Diabetic Retinopathy

Altered Expressions of Transfer RNA-Derived Small RNAs and microRNAs in the Vitreous Humor of Proliferative Diabetic Retinopathy

The Network of miRNA–mRNA Interactions in Circulating T Cells of Patients Following Major Trauma – A Pilot Study

Advances and Perspectives in Relation to the Molecular Basis of Diabetic Retinopathy—A Review

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