The agar microdilution method - a new method for antimicrobial susceptibility testing for essential oils and plant extracts

Golus, Joanna; Sawicki, Rafał; Widelski, Jarosław; Ginalska, Grażyna

doi:10.1111/jam.13253

Cited by 64 publications

(39 citation statements)

References 22 publications

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“…MIC was determined according to the method described by Golus et al [ 45 ]. Briefly, freshly prepared two-fold serial dilutions of the test essential oil (E.O.…”

Section: Methodsmentioning

confidence: 99%

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

et al. 2020

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Bacterial biofilm contributes to antibiotic resistance. Developing antibiofilm agents, more favored from natural origin, is a potential method for treatment of highly virulent multidrug resistant (MDR) bacterial strains; The potential of Pimenta dioica and Pimenta racemosa essential oils (E.Os) antibacterial and antibiofilm activities in relation to their chemical composition, in addition to their ability to treat Acinetobacter baumannii wound infection in mice model were investigated; P. dioica leaf E.O at 0.05 µg·mL−1 efficiently inhibited and eradicated biofilm formed by A. baumannii by 85% and 34%, respectively. Both P. diocia and P. racemosa leaf E.Os showed a bactericidal action against A. baumanii within 6h at 2.08 µg·mL−1. In addition, a significant reduction of A. baumannii microbial load in mice wound infection model was found. Furthermore, gas chromatography mass spectrometry analysis revealed qualitative and quantitative differences among P. racemosa and P. dioica leaf and berry E.Os. Monoterpene hydrocarbons, oxygenated monoterpenes, and phenolics were the major detected classes. β-Myrcene, limonene, 1,8-cineole, and eugenol were the most abundant volatiles. While, sesquiterpenes were found as minor components in Pimenta berries E.O; Our finding suggests the potential antimicrobial activity of Pimenta leaf E.O against MDR A. baumannii wound infections and their underlying mechanism and to be further tested clinically as treatment for MDR A. baumannii infections.

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“…MIC was determined according to the method described by Golus et al [ 45 ]. Briefly, freshly prepared two-fold serial dilutions of the test essential oil (E.O.…”

Section: Methodsmentioning

confidence: 99%

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

et al. 2020

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“…The minimum inhibitory concentration (MIC) was determined by microdilution method (Golus et al, 2016) using serially diluted (2-fold) of plant extract.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical standardization and biological activities of certain desert plants growing in Saudi Arabia

Al-Saleem

Awaad

Al‐Othman

et al. 2018

Saudi Pharmaceutical Journal

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The phytochemical screening, antimicrobial and antitumor activities of ,, ,, , and determined. The best antibacterial activity; 41.8 ± 0.23 mm, 39.7 ± 0.25 mm, 35.8 ± 0.58 mm, 34.7 ± 0.51 mm and 32.7 ± 0.25 mm was obtained by against , against , against , against and against , respectively. While the highest antifungal activity; 35.9 ± 1.15 mm, 34.6 ± 0.34, 30.6 ± 0.26 mm and 29.9 ± 0.63 mm was obtained by against ,, and, respectively. The antitumor activity (IC) obtained by ; 3.1 ± 6.9 µg/ml, 14.3 ± 3.1 µg/ml and 22.7 ± 4.1 µg/ml was better than activity of vinblastine sulphate; 5.9 ± 0.4 µg/ml, 59.7 ± 2.1 µg/ml and 30.3 ± 1.4 µg/ml against breast carcinoma (MCF-7), cervical carcinoma (Hela) and colorectal carcinoma (CACO), respectively. alcoholic extract showed higher antitumor activity; 15.3 ± 5.3 µg/ml, 28.6 ± 3.6 µg/ml and 24.3 ± 4.1 µg/ml than vinblastine; 21.2 ± 0.9 µg/ml, 59.7 ± 2.1 µg/ml and 30.3 ± 1.4 µg/ml against prostate carcinoma (Pc3), cervical carcinoma (Hela) and colorectal carcinoma (CACO), respectively. Also, the antitumor activity of against cervical carcinoma (Hela) (37.1 ± 2.6 µg/ml) was potent than vinblastine sulphate (59.7 ± 2.1 µg/ml). The obtained results of LD and sub-chronic toxicity revealed that the plants have no toxicity.

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“…The minimum inhibitory concentration (MIC) was defined as the lowermost concentration of compounds that did not show visible growth of bacteria. Extracts and compounds with MICs of less than or equal to 250 μg / mL were considered of concern Golus et al (2016).…”

Section: Extractionmentioning

confidence: 99%

Inhibition of Pathogenic Bacteria Isolated from Some Nuts Seeds by Natural and Some Commercial Coumarins

Aldayel¹

2020

SJKFU

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The antibacterial potential of a natural coumarin compound that was extracted from the root of Lotus lalambensis Schweinf and several commercial coumarin compounds (herniarin, xanthotoxin, umbelliferone and scopoletin) were investigated against five pathogenic bacteria (Staphylococcus epidermidis, Bacillus mycoides, Escherichia coli, Staphylococcus aureus and Bacillus cereus) that were isolated from different nut products. The natural coumarin showed the best antibacterial potential against the most sensitive bacteria (Staphylococcus aureus and Bacillus cereus) with a minimum inhibitory concentration of 62.5 μg ml-1. The antibacterial mechanism of different coumarin compounds was investigated by assessing the effect of coumarin on the membrane potential to quantify the alkaline phosphatase and lactate dehydrogenase activities of tested bacteria. The membrane potential of Staphylococcus aureus was higher in natural coumarin than in the control and commercial coumarin, followed by Bacillus cereus. The concentration of extracellular protein and the activity of lactate dehydrogenase and alkaline phosphtase were evaluated, and the bacteria that was treated with the natural coumarin had the highest antibacterial activity. The results showed the significant potential of coumarin in isolated DNA cleavage reactions. Our data also showed that the addition of natural coumarin and commercial coumarins resulted in a higher discharge of membrane potential in the supernatant. The conclusion revealed that the natural coumarin of Lotus lalambensis is potentially a significant source of useful molecules for the expansion of new antibacterial agents and the promising manipulation of natural coumarin to protect contaminated nuts and other foods against the pathogenic bacteria.

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The agar microdilution method - a new method for antimicrobial susceptibility testing for essential oils and plant extracts

Cited by 64 publications

References 22 publications

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

Phytochemical standardization and biological activities of certain desert plants growing in Saudi Arabia

Inhibition of Pathogenic Bacteria Isolated from Some Nuts Seeds by Natural and Some Commercial Coumarins

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