The Agaricus bisporus mannitol pathway during sporophore growth

N, Morton; Hammond, John B. W.; Dickerson, A. G.

doi:10.1016/s0007-1536(85)80262-x

Cited by 12 publications

(12 citation statements)

References 11 publications

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“…Although a functional mannitol cycle was present in P. ostreatus, F6P activity was detected in all the stages of development. The enzyme activity was low in sporulating fruit bodies as observed in A. bisporus and L. edodes [7,8].…”

Section: Fructose-6-phosphatasementioning

confidence: 99%

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The mannitol cycle in Pleurotus ostreatus (Florida)

Chakraborty

2004

FEMS Microbiology Letters

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In mushroom, presence of the mannitol cycle has not been reported so far although the polyol is supposed to be generated by the reduction of fructose by mannitol dehydrogenase. This study submits evidence for the presence of the mannitol cycle in Pleurotus ostreatus. The key enzyme of the cycle, mannitol-1-phosphate dehydrogenase (M1PDH), was present appreciably in all the developmental stages of the mushroom. However, the enzyme level dropped significantly at the onset of sporulation. The presence of M1DPH was confirmed by isozyme analysis and RT-PCR mediated amplification of a approximately 400 bp DNA fragment.

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Section: Fructose-6-phosphatasementioning

confidence: 99%

“…Synthesis of mannitol in fungi takes place through two established pathways. In one pathway, fructose is reduced to mannitol as reported for edible mushrooms, Agaricus bisporus and Lentinus edodes [8,9]. This reaction is catalysed by mannitol dehydrogenase (MtDH).…”

Section: Introductionmentioning

confidence: 99%

The mannitol cycle in Pleurotus ostreatus (Florida)

Chakraborty

2004

FEMS Microbiology Letters

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“…Although a functional mannitol cycle was present in P. ostreatus , F6P activity was detected in all the stages of development. The enzyme activity was low in sporulating fruit bodies as observed in A. bisporus and L. edodes [7,8].…”

Section: Resultsmentioning

confidence: 83%

The mannitol cycle in Pleurotus ostreatus (Florida)

Chakraborty

Basu

Das

et al. 2004

FEMS Microbiology Letters

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In mushroom, presence of the mannitol cycle has not been reported so far although the polyol is supposed to be generated by the reduction of fructose by mannitol dehydrogenase. This study submits evidence for the presence of the mannitol cycle in Pleurotus ostreatus. The key enzyme of the cycle, mannitol‐1‐phosphate dehydrogenase (M1PDH), was present appreciably in all the developmental stages of the mushroom. However, the enzyme level dropped significantly at the onset of sporulation. The presence of M1DPH was confirmed by isozyme analysis and RT‐PCR mediated amplification of a ∼400 bp DNA fragment.

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“…The first occurs via reduction of fructose, which is mediated by the enzyme mannitol dehydrogenase (8). Fructose may be generated from fructose-6-phosphate by the action of fructose-6-phosphatase (12). Second, synthesis may occur via dephosphorylation of mannitol-1-phosphate, which is mediated by mannitol-1-phosphatase.…”

mentioning

confidence: 99%

“…In basidiomycetes, the mannitol pathway was investigated in the button mushroom, Agaricus bisporus, during sporophore growth (12). Mannitol dehydrogenase activity was high in early stages of mushroom development and declined during sporophore growth to a very low level after sporulation.…”

mentioning

confidence: 99%

Mannitol Metabolism in Lentinus edodes , the Shiitake Mushroom

Kulkarni

1990

Appl Environ Microbiol

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Mannitol metabolism was evaluated in fruiting bodies of Lentinus edodes. Cell extracts were prepared from fruiting bodies, and key enzymes involved in mannitol metabolism were assayed, including hexokinase, mannitol dehydrogenase, mannitol-1-phosphate dehydrogenase, mannitol-1-phosphatase, and fructose-6phosphatase. Mannitol dehydrogenase, fructose-6-phosphatase, mannitol-1-phosphatase, and hexokinase activities were found in extracts of fruiting bodies. However, mannitol-1-phosphate dehydrogenase activity was not detected. Mycelial cultures were grown in an enriched liquid medium, and enzymes of the mannitol cycle were assayed in cell extracts of rapidly growing cells. Mannitol-1-phosphate dehydrogenase activity was also not found in mycelial extracts. Hence, evidence for a complete mannitol cycle both in vegetative mycelia and during mushroom development was lacking. The pathway of mannitol synthesis in L. edodes appears to utilize fructose as an intermediate.

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The Agaricus bisporus mannitol pathway during sporophore growth

Cited by 12 publications

References 11 publications

The mannitol cycle in Pleurotus ostreatus (Florida)

The mannitol cycle in Pleurotus ostreatus (Florida)

The mannitol cycle in Pleurotus ostreatus (Florida)

Mannitol Metabolism in Lentinus edodes , the Shiitake Mushroom

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