The aggressive spiegeldanio, carrying a mutation in the fgfr1a gene, has no advantage in dyadic fights with zebrafish of the AB strain

Mustafa, Arshi; Thörnqvist, Per‐Ove; Roman, Erika; Winberg, Svante

doi:10.1016/j.bbr.2019.111942

Cited by 8 publications

(10 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Norton et al (2011) also reported that in mirror tests, fgfr1a −/− mutants were more aggressive than Tübingen wild-type fish. Recently, it was shown that fgfr1a −/− males were more aggressive than AB males, but only if tested in a mirror test (Mustafa et al, 2019). In dyadic fights, fgfr1a −/− males were not more aggressive than AB males, and they had no advantage in fights for social dominance with size-matched AB males (Mustafa et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it was shown that fgfr1a −/− males were more aggressive than AB males, but only if tested in a mirror test (Mustafa et al, 2019). In dyadic fights, fgfr1a −/− males were not more aggressive than AB males, and they had no advantage in fights for social dominance with size-matched AB males (Mustafa et al, 2019). Boldness is often correlated with aggression, forming a behavioral syndrome (Sih et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Boldness in Male and Female Zebrafish (Danio rerio) Is Dependent on Strain and Test

Mustafa

Roman

Winberg

2019

Front. Behav. Neurosci.

Self Cite

View full text Add to dashboard Cite

Differences in selection pressure in nature and labs have profound effects on zebrafish strains. The widely used AB strain of zebrafish has been domesticated over several decades. Recently, there has been an upsurge in the availability of genetically modified lines, e.g., the spiegeldanio (spd), which has a mutation in the fibroblast growth factor receptor 1a (fgfr1a) gene. This mutant strain (fgfr1a) has previously been reported to be bolder than fish of the Tübingen strain, from which it was generated. Our knowledge on behavioral differences between different zebrafish strains, relative to wild-caught zebrafish, is limited. In the present study we compare behaviors related to interpretation of boldness in male and female offspring (F1) of wild-caught fish, AB and fgfr1a−/− zebrafish. A second aim of the study was to compare the behavior of fish from these strains when tested in different behavioral assays, i.e., shelter seeking, novel tank diving and scototaxis tests. The results demonstrate that behavioral variation exists both within and between the strains, but interpretation of boldness reveals a complex pattern in which behavior differs between strains but is also related to sex and test. Therefore, a careful assessment of various strains of fish using both males and females is warranted in order to strengthen interpretation of results. This is especially important in studies where zebrafish are used as model organisms for human conditions as well as studies evaluating the effects of pharmacological or toxicological substances on behavior.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Boldness in Male and Female Zebrafish (Danio rerio) Is Dependent on Strain and Test

Mustafa

Roman

Winberg

2019

Front. Behav. Neurosci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Wildtype (AB) zebrafish were purchased from the China Zebrafish Resource Center, and bred in the zebrafish circulating aquaculture system (ESEN, China) according to Mustafa et al [ 42 ]. Larval zebrafish (5 days post-fertilization) with normal development were selected under a stereomicroscope and randomly placed in 6-well plates containing 3 mL of culture medium, with 10 fishes in each well.…”

Section: Methodsmentioning

confidence: 99%

Bioevaluation of Pheretima vulgaris Antithrombotic Extract, PvQ, and Isolation, Identification of Six Novel PvQ-Derived Fibrinolytic Proteases

Yang

Wang

et al. 2021

Molecules

View full text Add to dashboard Cite

Thrombosis is a disease that seriously endangers human health, with a high rate of mortality and disability. However, current treatments with thrombolytic drugs (such as recombinant tissue-plasminogen activator) and the oral anticoagulants (such as dabigatran and rivaroxaban) are reported to have a tendency of major or life-threatening bleeding, such as intracranial hemorrhage or massive gastrointestinal bleed with non-specific antidotes. In contrast, lumbrokinase is very specific to fibrin as a substrate and does not cause excessive bleeding. It can dissolve the fibrin by itself or convert plasminogen to plasmin by inducing endogenous t-PA activity to dissolve fibrin clots. Therefore, searching for potentially new therapeutic molecules from earthworms is significant. In this study, we first collected a strong fibrinolytic extract (PvQ) from the total protein of the Pheretima vulgaris with AKTA pure protein purification systems; its fibrinolytic bioactivity was verified by the fibrin plate assay and zebrafish thrombotic model of vascular damage. Furthermore, according to the cell culture model of human umbilical vein endothelial cells (HUVECs), the PvQ was proven to exhibit the ability to promote the secretion of tissue-type plasminogen activator (t-PA), which further illustrated that it has an indirect thrombolytic effect. Subsequently, extensive chromatographic techniques were applied to reveal the material basis of the extract. Fortunately, six novel earthworm fibrinolytic enzymes were obtained from the PvQ, and the primary sequences of those functional proteins were determined by LC-MS/MStranscriptome cross-identification and the Edman degradation assay. The secondary structures of these six fibrinolytic enzymes were determined by circular dichroism spectroscopy and the three-dimensional structures of these proteases were predicted by MODELLER 9.23 based on multi-template modelling. In addition, those six genes encoding blood clot-dissolving proteins were cloned from P. vulgaris by RT-PCR amplification, which further determined the accuracy of proteins primary sequences identifications and laid the foundation for subsequent heterologous expression.

show abstract

“…The aforementioned spiegeldanio zebrafish mutant sheds some light on the aggression-exploration syndrome (Mustafa, Roman, et al, 2019 ; Mustafa, Thörnqvist, Roman, & Winberg, 2019 ; Norton et al, 2011 ; Norton & Bally-Cuif, 2012 ). As described above, spiegeldanio show increased aggressiveness in a mirror test (Mustafa, Thörnqvist, et al, 2019 ; Norton et al, 2011 ), an effect that has been attributed to population-level differences in histaminergic signaling; this aggressiveness does not translate into more success in dyadic fights, however, as fgfr1a mutant fish did not have any advantage in fights for social dominance, and agonistic behavior of these mutants did not differ from that of AB fish during dyadic interactions (Mustafa, Thörnqvist, et al, 2019 ). Thus, the aggression-exploration syndrome of fgfr1a mutants is not associated with social plasticity, the ability to rapidly switch between behaviors in response to changing social conditions (Taborsky & Oliveira, 2012 ).…”

Section: Dimensionality Of Personality In Fishmentioning

confidence: 99%

Fish personality: meta-theoretical issues, personality dimensions, and applications to neuroscience and psychopathology

Luchiari,

Maximino

2023

Personality Neuroscience

View full text Add to dashboard Cite

While the field of personality neuroscience has extensively focused on humans and, in a few cases, primates and rodents, a wide range of research on fish personality has emerged in the last decades. This research is focused mainly on the ecological and evolutionary causes of individual differences and also aimed less extensively at proximal mechanisms (e.g., neurochemistry or genetics). We argue that, if consistent and intentional work is made to solve some of the meta-theoretical issues of personality research both on fish and mammals, fish personality research can lead to important advances in personality neuroscience as a whole. The five dimensions of personality in fish (shyness-boldness, exploration-avoidance, activity, aggressiveness, and sociability) need to be translated into models that explicitly recognize the impacts of personality in psychopathology, synergizing research on fish as model organisms in experimental psychopathology, personality neuroscience, and ecological-ethological approaches to the evolutionary underpinnings of personality to produce a powerful framework to understand individual differences.

show abstract

The aggressive spiegeldanio, carrying a mutation in the fgfr1a gene, has no advantage in dyadic fights with zebrafish of the AB strain

Cited by 8 publications

References 25 publications

Boldness in Male and Female Zebrafish (Danio rerio) Is Dependent on Strain and Test

Boldness in Male and Female Zebrafish (Danio rerio) Is Dependent on Strain and Test

Bioevaluation of Pheretima vulgaris Antithrombotic Extract, PvQ, and Isolation, Identification of Six Novel PvQ-Derived Fibrinolytic Proteases

Fish personality: meta-theoretical issues, personality dimensions, and applications to neuroscience and psychopathology

Contact Info

Product

Resources

About