The Agrobacterium-Plant Cell Interaction. Taking Biology Lessons from a Bug

Tzfira, Tzvi; Citovsky, Vitaly

doi:10.1104/pp.103.032821

Cited by 38 publications

(15 citation statements)

References 62 publications

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“…Several investigators have recently suggested that particle bombardment will inevitably be supplanted by transformation methods using the soil bacterium Agrobacterium tumefaciens and its relatives, at least for the production of commercial genetically enhanced crops (Gelvin 2003;Tzfira and Citovsky 2003;Valentine 2003). The subject of genetically enhanced crops has ignited an intense, polarized and often hostile debate in many countries, with emotive issues taking the lead over scientific rigor and common sense.…”

Section: Introductionmentioning

confidence: 99%

Particle bombardment and the genetic enhancement of crops: myths and realities

et al. 2005

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DNA transfer by particle bombardment makes use of physical processes to achieve the transformation of crop plants. There is no dependence on bacteria, so the limitations inherent in organisms such as Agrobacterium tumefaciens do not apply. The absence of biological constraints, at least until DNA has entered the plant cell, means that particle bombardment is a versatile and effective transformation method, not limited by cell type, species or genotype. There are no intrinsic vector requirements so transgenes of any size and arrangement can be introduced, and multiple gene cotransformation is straightforward. The perceived disadvantages of particle bombardment compared to Agrobacterium-mediated transformation, i.e. the tendency to generate large transgene arrays containing rearranged and broken transgene copies, are not borne out by the recent detailed structural analysis of transgene loci produced by each of the methods. There is also little evidence for major differences in the levels of transgene instability and silencing when these transformation methods are compared in agriculturally important cereals and legumes, and other non-model systems. Indeed, a major advantage of particle bombardment is that the delivered DNA can be manipulated to influence the quality and structure of the resultant transgene loci. This has been demonstrated in recently reported strategies that favor the recovery of transgenic plants containing intact, singlecopy integration events, and demonstrating high-level transgene expression. At the current time, particle bombardment is the most efficient way to achieve plastid transformation in plants and is the only method so far used to achieve mitochondrial transformation. In this review, we discuss recent data highlighting the positive impact of particle bombardment on the genetic transformation of plants, focusing on the fate of exogenous DNA, its organization and its expression in the plant cell. We also discuss some of the most important applications of this technology including the deployment of transgenic plants under field conditions.

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Section: Introductionmentioning

confidence: 99%

Particle bombardment and the genetic enhancement of crops: myths and realities

et al. 2005

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“…The T-strand is wrapped with numerous VirE2 proteins to produce a mature transfer complex (T-complex) (3), which is then imported into the host cell nucleus. Additional bacterial proteins and host factors are involved in genomic integration and expression of the encoded genes (1)(2)(3)(4)(5)(6).…”

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confidence: 99%

Plant Transformation by Agrobacterium tumefaciens

Frenkiel-Krispin

Wolf

Albeck

et al. 2007

Journal of Biological Chemistry

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Agrobacterium tumefaciens infects plant cells by the transfer of DNA. A key factor in this process is the bacterial virulence protein VirE2, which associates stoichiometrically with the transported single-stranded (ss) DNA molecule (T-strand). As observed in vitro by transmission electron microscopy, VirE2-ssDNA readily forms an extended helical complex with a structure well suited to the tasks of DNA protection and nuclear import. Here we have elucidated the role of the specific molecular chaperone VirE1 in regulating VireE2-VirE2 and VirE2-ssDNA interactions. VirE2 alone formed functional filamentous aggregates capable of ssDNA binding. In contrast, co-expression with VirE1 yielded monodisperse VirE1-VirE2 complexes. Cooperative binding of VirE2 to ssDNA released VirE1, resulting in a controlled formation mechanism for the helical complex that is further promoted by macromolecular crowding. Based on this in vitro evidence, we suggest that the constrained volume of the VirB channel provides a natural site for the exchange of VirE2 binding from VirE1 to the T-strand.

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“…The Agrobacterium-based DNA transformation method offers many unique advantages: (1) precise integration of genes with defined end, (2) transfer of desired DNA along with the marker gene, (3) high frequency of stable and intact gene transfer, and (4) ability to transfer long-stretch DNA (>150 kb) and reduced rate of transgene silencing (Tzfira and Citovsky 2003). Although, Agrobacterium-mediated gene transfer is a widely and commonly used method, it has several drawbacks (Tzfira and Citovsky 2003;Shrawat and Good 2011). These include (1) limitation to carry size base pair (<500 kb), (2) chances of transgene silencing, (3) poor gene transfer efficiency, and (4) effectiveness against dicot plants.…”

Section: Conventional Biotransformation Technologies In Plantsmentioning

confidence: 99%

Nanoparticles-Based Delivery Systems in Plant Genetic Transformation

Rai

Bansod

Bawaskar

et al. 2015

Nanotechnologies in Food and Agriculture

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The production of transgenic plants is considered as a valuable tool in plant research and the technology is comprehensively useful in agricultural research. Gene transfer in plants is generally carried out by Agrobacterium sp., application of some chemicals, and physical techniques (electroporation, microprojectile, etc.). Now-a-days with better efficacy and stability, new methods for the gene transfer in plants are coming up. The advent of nanotechnology, the nanoparticles-based delivery systems for genetic transformation of plants, is coming in a big way.In this chapter, we have discussed the novel nanotechnologies like nucleic acidconjugated nanoparticles with their current status and future prospects in the development of gene transfer methods in plants. We have also highlighted the shortcomings of conventional techniques of gene transfer in plants and discussed the role of established nanotechnology and chemical-based strategy for surface

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The Agrobacterium-Plant Cell Interaction. Taking Biology Lessons from a Bug

Cited by 38 publications

References 62 publications

Particle bombardment and the genetic enhancement of crops: myths and realities

Particle bombardment and the genetic enhancement of crops: myths and realities

Plant Transformation by Agrobacterium tumefaciens

Nanoparticles-Based Delivery Systems in Plant Genetic Transformation

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