The AKT2 potassium channel mediates NaCl induced depolarization in the root of<i>Arabidopsis thaliana</i>

Salvador-Recatalà, Vicenta

doi:10.1080/15592324.2016.1165381

Cited by 13 publications

(14 citation statements)

References 16 publications

(15 reference statements)

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“…In Spartina , the reduction in expression of SaAKT2 but no change of SaAKT1 (Table S9 ) may limit the K + efflux under high salt stress and the results supported the physiologic observation that Spartina has a higher K + accumulation level (Hester et al, 2001; Subudhi & Baisakh, 2011). Moreover, the down-regulation of SaAKT2 may also reduce the Na + uptake root cells initially through the K + channel via SaAKT2 (Salvador-Recatala, 2016). KAT2 and KAT1 have been reported to form inward K + channels in the form of heterodimers and KAT2 can also form active homotrimeric channels in the plasma membrane (Nieves-Cordones et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

The full-length transcriptome of Spartina alterniflora reveals the complexity of high salt tolerance in monocotyledonous halophyte

Fraser

Wang

Wei

et al. 2019

Preprint

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18 Spartina alterniflora (Spartina) is the only halophyte in the salt marsh. However, the 19 molecular basis of its high salt tolerance remains elusive. In this study, we used 20 PacBio full-length single molecule long-read sequencing and RNA-seq to elucidate 21 the transcriptome dynamics of high salt tolerance in Spartina by salt-gradient 22 Spartina. Overall, this study sheds light on the high salt tolerance mechanisms of 34 monocotyledonous-halophyte and demonstrates the potential of Spartina genes for 35 engineering salt-tolerant plants. 36 Running title: Spartina transcriptome under salt stress 37 Keywords: Spartina alterniflora; high salt tolerance; ion transporter; protein kinase; 38 regulatory hub genes; alternative splicing; single molecule real-time sequencing 39 40 41 42 43 44 45 46 47 48 alterniflora exhibits a delicate ion exclusion system from physiology aspects to 66 survive hash saline environment and it is a good model plant to study high salt 67 tolerance. However, the molecular basis of salt tolerance in Spartina alterniflora 68 remains unexplored, and the post-transcriptional regulation of high salt tolerance has 69 not been characterized in plants. 70

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Section: Discussionmentioning

confidence: 99%

The full-length transcriptome of Spartina alterniflora reveals the complexity of high salt tolerance in monocotyledonous halophyte

Fraser

Wang

Wei

et al. 2019

Preprint

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“…In the research of changes in excitability in sunflowers, we used KCl and NaCl. A treatment with the KCl solution was applied because K + is an important component of the AP mechanism and transmembrane potential maintenance (Trebacz et al, 1989 , 1994 ; Shabala, 2003 ; Johansson et al, 2006 ; Shabala and Cuin, 2008 ; Aleman et al, 2011 ; Dreyer and Uozumi, 2011 ; Shabala and Pottosin, 2014 ; Salvador-Recatala, 2016 ). In turn, NaCl was used because it is the most commonly occurring salt in the environmental salt stress (Munns and Tester, 2008 ; Maathuis, 2014 ; Foster and Miklavcic, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…This shows that only the mild stress induced by KCl increases the appearance of SAPs in sunflower seedlings. Potassium ions are an important part of the mechanism of maintaining the excitability of the cell membrane and we can assume that its slight increase in the environment affects the membrane transport (Trebacz et al, 1989 , 1994 ; Shabala, 2003 ; Johansson et al, 2006 ; Shabala and Cuin, 2008 ; Shabala and Pottosin, 2014 ; Salvador-Recatala, 2016 ). The mild stress induced by NaCl slightly increases the number of SAPs and increases SAP velocity in a statistically significant way.…”

Section: Discussionmentioning

confidence: 99%

“…A treatment with KCl combined with prior pricking induced series of APs in C. conicum (Favre et al, 1999 ) and A. thaliana (Favre et al, 2011 ). Recently, Salvador-Recatala ( 2016 ) used NaCl and KCl solutions to evoke depolarization of root cells of A. thaliana . The salts were also administered in Vicia faba and Hordeum vulgare to evoke system potentials (SPs) (Zimmermann et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

“…Complete electrophysiological characterization of APs (threshold, refractory periods, “all-or-none” law, velocity of propagation, chronaxie, reobase) has been carried out for Helianthus annuus, Lupinus angustifolius , and Conocephalum conicum (Paszewski and Zawadzki, 1973 , 1974 , 1976a , b ; Zawadzki, 1979 , 1980 ; Zawadzki et al, 1991 , 1995 ; Favre et al, 1999 ). It is known that APs are involved in rapid plant movement and regulate many physiological processes and the circumstances of their appearance are still studied (Sibaoka, 1991 ; Stankovic et al, 1998 ; Dziubinska, 2003 ; Stahlberg et al, 2006 ; Zimmermann et al, 2009 , 2016 ; Król et al, 2010 ; Stolarz et al, 2010 ; Salvador-Recatala et al, 2014 ; van Bel et al, 2014 ; Kiep et al, 2015 ; Macedo et al, 2015 ; Salvador-Recatala and Tjallingii, 2015 ; Hedrich et al, 2016 ; Salvador-Recatala, 2016 ). A spontaneous action potential (SAP) is an action potential in which exogenous or endogenous stimuli evoking them are not known.…”

Section: Introductionmentioning

confidence: 99%

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Osmotic and Salt Stresses Modulate Spontaneous and Glutamate-Induced Action Potentials and Distinguish between Growth and Circumnutation in Helianthus annuus Seedlings

Stolarz

Dziubińska

2017

Front. Plant Sci.

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Action potentials (APs), i.e., long-distance electrical signals, and circumnutations (CN), i.e., endogenous plant organ movements, are shaped by ion fluxes and content in excitable and motor tissues. The appearance of APs and CN as well as growth parameters in seedlings and 3-week old plants of Helianthus annuus treated with osmotic and salt stress (0–500 mOsm) were studied. Time-lapse photography and extracellular measurements of electrical potential changes were performed. The hypocotyl length was strongly reduced by the osmotic and salt stress. CN intensity declined due to the osmotic but not salt stress. The period of CN in mild salt stress was similar to the control (~164 min) and increased to more than 200 min in osmotic stress. In sunflower seedlings growing in a hydroponic medium, spontaneous APs (SAPs) propagating basipetally and acropetally with a velocity of 12–20 cm min−1 were observed. The number of SAPs increased 2–3 times (7–10 SAPs 24 h−1plant−1) in the mild salt stress (160 mOsm NaCl and KCl), compared to the control and strong salt stress (3–4 SAPs 24 h−1 plant−1 in the control and 300 mOsm KCl and NaCl). Glutamate-induced series of APs were inhibited in the strong salt stress-treated seedlings but not at the mild salt stress and osmotic stress. Additionally, in 3-week old plants, the injection of the hypo- or hyperosmotic solution at the base of the sunflower stem evoked series of APs (3–24 APs) transmitted along the stem. It has been shown that osmotic and salt stresses modulate differently hypocotyl growth and CN and have an effect on spontaneous and evoked APs in sunflower seedlings. We suggested that potassium, sodium, and chloride ions at stress concentrations in the nutrient medium modulate sunflower excitability and CN.

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Spontaneous action potentials and circumnutation in Helianthus annuus

Stolarz

Dziubińska

2017

Acta Physiol Plant

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Action potentials (APs) in plants are involved in fast leaf or trap closure as well as elongation, respiration, photosynthesis, and fertilization regulation. Here, spontaneous APs (SAPs) in relation to endogenous stem movement named circumnutation (CN) have been investigated in Helianthus annuus in different light conditions in freely circumnutating and immobilized plants. Extracellular electrical measurements and time-lapse photography were carried out simultaneously. The parameters of CN (trajectory length, period, and direction) and the number and transmission direction of SAPs were analysed. In continuous light (25-40 lmol m -2 s -1), all plants circumnutating vigorously in a regular elliptical manner and no SAPs were observed. In light/dark conditions, the plants circumnutated in a daily pattern, most SAPs were observed in the dark and freely circumnutated sunflowers had two times more SAPs (10 SAPs/24 h/plant) than the immobilized plants (5 SAPs/24 h/plant). In continuous very low light (5 lmol m -2 s -1 ), the plants circumnutated weakly and irregularly and SAPs appeared without the circadian pattern. 3-5 SAPs/24/plant occurred in the freely circumnutating and immobilized plants. In light/dark and continuous very low light conditions, an ultradian rhythm of SAPs was observed and the mean spacing between SAPs was approx. 121-271 min in the freely circumnutating and immobilized plants. Under all light conditions, more SAPs were transmitted basipetally than acropetally. One-hour lasting series of 3-4 min spaced SAPs locally propagated were observed as well in very low light. Basipetal and acropetal SAPs passing along the stem motor region accompany irregularity, changes in the CN trajectory direction, and stem torsion. These results demonstrate that APs and CN changes play a role in plant adaptation to light conditions and that there is an ultradian rhythm of SAPs beside ultradian CN rhythm.

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The AKT2 potassium channel mediates NaCl induced depolarization in the root ofArabidopsis thaliana

Cited by 13 publications

References 16 publications

The full-length transcriptome of Spartina alterniflora reveals the complexity of high salt tolerance in monocotyledonous halophyte

The full-length transcriptome of Spartina alterniflora reveals the complexity of high salt tolerance in monocotyledonous halophyte

Osmotic and Salt Stresses Modulate Spontaneous and Glutamate-Induced Action Potentials and Distinguish between Growth and Circumnutation in Helianthus annuus Seedlings

Spontaneous action potentials and circumnutation in Helianthus annuus

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