The Allergenic Activity and Stability of Purified Allergens from the Pollen of Common Rye Grass (Lolium perenne)

Marsh, David G.; Milner, F. H.; Johnson, Phil

doi:10.1159/000229739

Cited by 59 publications

(25 citation statements)

References 1 publication

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“…The dose-response relationships of histamine and arginine esterase release have been studied with leukocyte preparations from 25 allergic and 23 normal individuals challenged with either the purified protein antigens from ragweed (AgE) (18) or grass (19) or with highly specific anti-IgE (20). An arginine esterase dose-response curve is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Anaphylactic relase of a basophil kallikrein-like activity. II. A mediator of immediate hypersensitivity reactions.

Newball¹,

Talamo²,

Lichtenstein³

1979

J. Clin. Invest.

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A B S T R A C T This report describes the immune release of a new mediator from human peripheral leukocytes, a basophil kallikrein-like activity (BK-A). The release process is initiated by the interaction of antigen or anti-IgE with cell-bound IgE, and appears to be similar in mechanism to the release of histamine and other mediators of the immediate hypersensitivity reaction. The dose-response relationships arid kinetics ofhistamine and BK-A release from antigen-challenged peripheral leukocytes are similar. The release of the BK-A is calcium and temperature dependent, requires metabolic energy, and is controlled by hormone-receptor interactions that influence the cellular level of cyclic AMP, as has been described for other mediators of immediate hypersensitivity reactions. The data indicate that the interaction of BK-A with human plasma kininogen, generates immunoreactive kinin. We conclude that the antigen-IgE interaction leads to the release from human basophils of a new mediator, a basophil kallikrein-like activity which may well be a link between reactions of immediate hypersensitivity and the plasma and/or tissue kinin-generating systems.

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Section: Resultsmentioning

confidence: 99%

Anaphylactic relase of a basophil kallikrein-like activity. II. A mediator of immediate hypersensitivity reactions.

Newball¹,

Talamo²,

Lichtenstein³

1979

J. Clin. Invest.

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“…Allergies to grass pollen are widespread, afflicting an estimated 200-400 million people, and numerous studies have concluded that the group-1 allergens are the most important allergenic components of grass pollen (23,23,54,55). Maize EXPB1 and its orthologs in turf grasses share common epitopes, as judged by antibody cross-reactivity, with the predominant epitopes found in the protein portion of the molecule and the glycosyl residues being of secondary antigenic significance (52,56,57).…”

Section: Expansins As Cysteine Proteases?mentioning

confidence: 99%

Crystal structure and activities of EXPB1 (Zea m 1), a β-expansin and group-1 pollen allergen from maize

Yennawar¹,

Li²,

Dudzinski

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

232

247

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Expansins are small extracellular proteins that promote turgordriven extension of plant cell walls. EXPB1 (also called Zea m 1) is a member of the ␤-expansin subfamily known in the allergen literature as group-1 grass pollen allergens. EXPB1 induces extension and stress relaxation of grass cell walls. To help elucidate expansin's mechanism of wall loosening, we determined the structure of EXPB1 by x-ray crystallography to 2.75-Å resolution. EXPB1 consists of two domains closely packed and aligned so as to form a long, shallow groove with potential to bind a glycan backbone of Ϸ10 sugar residues. The structure of EXPB1 domain 1 resembles that of family-45 glycoside hydrolase (GH45), with conservation of most of the residues in the catalytic site. However, EXPB1 lacks a second aspartate that serves as the catalytic base required for hydrolytic activity in GH45 enzymes. Domain 2 of EXPB1 is an Ig-like ␤-sandwich, with aromatic and polar residues that form a potential surface for polysaccharide binding in line with the glycan binding cleft of domain 1. EXPB1 binds to maize cell walls, most strongly to xylans, causing swelling of the cell wall. Tests for hydrolytic activity by EXPB1 with various wall polysaccharides proved negative. Moreover, GH45 enzymes and a GH45-related protein called ''swollenin'' lacked wall extension activity comparable to that of expansins. We propose a model of expansin action in which EXPB1 facilitates the local movement and stress relaxation of arabinoxylancellulose networks within the wall by noncovalent rearrangement of its target.plant cell wall

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“…Ragweed antigen E was provided by Dr. T. P. King (22) and rye grass group I antigen by Dr. D. G. Marsh (23). D20 was obtained from BioRad Laboratories (Richmond, Calif.), demecolcine (Colcemid) from Ciba Pharmaceutical Co. (Summit, N.…”

Section: Introductionmentioning

confidence: 99%

Histamine release from human leukocytes: studies with deuterium oxide, colchicine, and cytochalasin B

Gillespie¹,

Lichtenstein²

1972

J. Clin. Invest.

178

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A B S T R A C T Agents known to interact with either microtubules or microfilaments influenced the antigen-induced release of histamine from the leukocytes of allergic individuals. Deuterium oxide (D20) which stabilizes microtubules and thereby favors their formation enhanced histamine release markedly. Concentrations as low as 5% increased antigen-induced release somnewhat while concentrations as high as 75% had no effect on release in the absence of antigen. Enhancement occurred over a wide range of antigen concentrations and was also seen when release was initiated by antibody to IgE or IgG. When the release process was divided into two stages a D20 activity could be demonstrated only in the second stage. However, when D20 was present in the first stage together with agents which raise cyclic AMP levels and thereby inhibit release it partially reversed this inhibition. Colchicine, demecolcine, and vinblastine, compounds known to disaggregate microtubules, i.e., have an effect opposite to that of D20, inhibited the release of histamine and counteracted the effects of D20. The inhibitory action of colchicine was greater if cells were treated with colchicine before rather than after activation with antigen. Cytochalasin B, a compound which causes the disappearance of microfilaments, had variable effects on histamine release. The most frequently seen response was slight enhancement. Neither D20 nor cytochalasin B altered cyclic AMP levels in leukocytes. These observations support and strengthen the view that an intact and functioning microtubule system is directly important for the secretion of histamine from leukocytes and suggest that microfilaments might have multiple indirect effects.

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The Allergenic Activity and Stability of Purified Allergens from the Pollen of Common Rye Grass (Lolium perenne)

Cited by 59 publications

References 1 publication

Anaphylactic relase of a basophil kallikrein-like activity. II. A mediator of immediate hypersensitivity reactions.

Anaphylactic relase of a basophil kallikrein-like activity. II. A mediator of immediate hypersensitivity reactions.

Crystal structure and activities of EXPB1 (Zea m 1), a β-expansin and group-1 pollen allergen from maize

Histamine release from human leukocytes: studies with deuterium oxide, colchicine, and cytochalasin B

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