The Alleviative Effect of Vitamin B₂ on Potassium Bromate-Induced Hepatotoxicity in Male Rats

Abstract

Potassium bromate (PB) is a food enhancer, water disinfection by-product, and a proven carcinogen. It elicits toxicities in the living organism due to exposure and in a dose-dependent manner. The present study discourses the ameliorative efficacy of riboflavin (RF) in PB-administered rodents. The animals were distributed into five treatment groups: control (group I), PB alone (group II, 150 mg/kg), RF alone (group III, 2 mg/kg), PB+RF1 (group IV, 150 mg/kg+2 mg/kg), and PB+RF2 (group V, 150 mg/kg+4 mg/kg). After the round of the treatment, the animals were sacrificed to collect their blood and liver samples for the detailed analysis. Group II depicted perturbed liver functions evidenced by altered serum and toxicity markers along with the disturbed redox balance. Also, these biochemical results were found harmonious with histopathological analysis and comet assay. However, group III showed no noticeable alteration in the same parameters, whereas the combination groups (IV and V) exhibited dose-dependent amelioration in the PB-induced toxicities. Interestingly, RF favored apoptosis concomitant with suppressing the necrosis in the PB-challenged groups, as shown by the activity of caspase-3 and lactate dehydrogenase. Histopathological analysis and comet assay further consolidate these results. Hence, RF has significant alleviative property against PB-induced hepatotoxicity in vivo that can be used in the consumer items containing the toxicant.

Study the Protective Effect of Vitamin E Against Potassium Bromate Toxicity on Some Hematological, Renal, and Hepatic Functions in Male Rats

“…So, the elevation of liver enzymes activities may be due to the failure of parenchymal cells of the liver under pathological conditions to ensure the vital functions, causing distribution of metabolism. As, it was shown that KBrO 3 can induce hepatic impairment 45 . Herein, we found that VE treatment together with KBrO 3 alleviated the elevated activities of the markers of hepatocellular injury AST, ALT and ALP comparing with KBrO 3 treated rats.…”

Hepatocellular Injury Ameliorated by a Common African Food, Parkia biglobosa

“…Similar results were obtained in a study by Hassan et al [31] where rats given potassium bromate displayed elevated ALT, AST, and ALP activity. Numerous risk factors, such as potassium bromate, alcohol, environmental contaminants, and medications cause oxidative stress on liver cells [25,32]. The use of natural antioxidants found in medicinal plants has the power to scavenge free radicals created by oxidative stress, hence lessening liver damage caused by reactive oxygen species (ROS) [32].…”

“…Numerous risk factors, such as potassium bromate, alcohol, environmental contaminants, and medications cause oxidative stress on liver cells [25,32]. The use of natural antioxidants found in medicinal plants has the power to scavenge free radicals created by oxidative stress, hence lessening liver damage caused by reactive oxygen species (ROS) [32]. One of these therapeutic plants with antioxidant properties is Parkia biglobosa, which is an everyday diet in Africa [16,33].…”

Silver Nanoparticles Decorated with Curcumin Enhance the Efficacy of Metformin in Diabetic Rats via Suppression of Hepatotoxicity

“…The blood and liver tissue samples were prepared after sacrificing, as previously reported [32,33]. The serum samples were retrieved by centrifuging at 1000× g while the supernatants from tissue samples were retrieved from 3000× g. The samples were kept at −80 • C (Eppendorff, Stevenage, UK) till further analysis [30].…”