The alleviative effects of green synthesized copper oxide nanoparticles against oxidative stress, hepato-renal alterations, and immune suppression induced by Staphylococcus aureus infection in Clarias gariepinus

Rahman, Afaf N. Abdel; Abdelwarith, Abdelwahab A.; Younis, Elsayed M.; Rhouma, Nasreddin R.; Zaki, Haidy T.; Khalil, Samah S.; El-Saber, Mahmoud M.; Davies, Simon J.; El-Murr, Abdelhakeem; Ibrahim, Rowida E.

doi:10.1016/j.aqrep.2023.101746

Cited by 6 publications

(2 citation statements)

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“…Therefore, our results indicated kidney dysfunction as the renal markers, particularly serum creatinine, is mostly a diagnostic tool for kidney function. The same results were achieved by Rahman et al [60], who showed that S. aureus challenge increased the kidney function markers (creatinine and urea). In addition, Baraaj [64] stated that the blood collected from male rabbits injected by P. aeruginosa showed an elevation of plasma creatinine and urea levels compared with the control group.…”

Section: Discussionsupporting

confidence: 82%

“…In the present study, serum biochemical analysis for liver functions revealed a significant increase in ALT and AST activities as well as a significant decrease in serum albumin levels in P. aeruginosa and S. aureus-infected rats compared to the negative control. The same results were achieved by Rahman et al [60], who showed that the S. aureus challenge lowered the protein profile indices and elevated the liver enzyme activities. The S. aureus toxins may be responsible for destroying the hepatocytes.…”

Section: Discussionsupporting

confidence: 81%

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Chitosan-Loaded Lagenaria siceraria and Thymus vulgaris Potentiate Antibacterial, Antioxidant, and Immunomodulatory Activities against Extensive Drug-Resistant Pseudomonas aeruginosa and Vancomycin-Resistant Staphylococcus aureus: In Vitro and In Vivo Approaches

Taha,

Abd El-Aziz,

Abdelkhalek

et al. 2024

Antioxidants

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Antimicrobial resistance poses considerable issues for current clinical care, so the modified use of antimicrobial agents and public health initiatives, coupled with new antimicrobial approaches, may help to minimize the impact of multidrug-resistant (MDR) bacteria in the future. This study aimed to evaluate the antimicrobial, antioxidant, and immunomodulatory activities of Lagenaria siceraria, Thymus vulgaris, and their chitosan nanocomposites against extensive drug-resistant (XDR) Pseudomonas aeruginosa and vancomycin-resistant Staphylococcus aureus (VRSA) using both in vitro and in vivo assays. The in vitro antimicrobial susceptibilities of P. aeruginosa and VRSA strains revealed 100% sensitivity to imipenem (100%). All P. aeruginosa strains were resistant to cefoxitin, cefepime, trimethoprim + sulfamethoxazole, and fosfomycin. However, S. aureus strains showed a full resistance to cefoxitin, amoxicillin, ampicillin, erythromycin, chloramphenicol, and fosfomycin (100% each). Interestingly, all S. aureus strains were vancomycin-resistant (MIC = 32–512 μg/mL), and 90% of P. aeruginosa and S. aureus strains were XDR. The antimicrobial potential of Lagenaria siceraria and Thymus vulgaris nanocomposites with chitosan nanoparticles demonstrated marked inhibitory activities against XDR P. aeruginosa and VRSA strains with inhibition zones’ diameters up to 50 mm and MIC values ranging from 0.125 to 1 μg/mL and 1 to 8 μg/mL, respectively. The results of the in vivo approach in male Sprague Dawley rats revealed that infection with P. aeruginosa and S. aureus displayed significant changes in biochemical, hematological, and histopathological findings compared to the negative control group. These values returned to the normal range after treatment by chitosan nanoparticles, either loaded with Lagenaria siceraria or Thymus vulgaris. Real-time quantitative polymerase chain reaction (RT-qPCR) findings presented significant upregulation of the relative expression of the IL10 gene and downregulation of the IFNG gene throughout the experimental period, especially after treatment with chitosan nanoparticles loaded either with Lagenaria siceraria or Thymus vulgaris in comparison to the positive control groups. In conclusion, this is the first report suggesting the use of Lagenaria siceraria and Thymus vulgaris nanocomposites with chitosan nanoparticles as a promising contender for combating XDR P. aeruginosa and VRSA infections as well as a manager for inflammatory situations and oxidative stress-related disorders.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 81%