The allograft valve in heart transplantation and valve replacement: Genetic assessment of the origin of the cells by means of deoxyribonucleic acid profiles

Melo, João; Monteiro, Carolino; Neves, José Pedro; Santos, R R; Martins, Ana Paula; Ramos, Sância; Calta, Carol; Matoso-Ferreira, A; Viana, João Faro; Rueff, José; Machado-Macedo, Manuel

doi:10.1016/s0022-5223(95)70382-9

Cited by 7 publications

(4 citation statements)

References 10 publications

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“…However, there have been some reports about the immunogenicity of cryopreserved allografts. Some studies comparing homografts and the valves of transplanted hearts showed that leaflet cellularity of transplanted hearts is superior to that of homografts (5,6,7). These differences were consistent with the occurrence of an immune response in the homografts which was prevented or abrogated by immunosuppressive therapy administered to the transplanted hearts.…”

Section: Discussionmentioning

confidence: 83%

“…Homografts explanted up to 10 years after implantation have revealed the presence of intact donor fibroblasts according to DNA fingerprint studies (1). Although other studies have revealed that the homograft was acellular (5–7) compared to the valve of a transplanted heart, previous investigations of donor‐specific immune responses in human and in animal models (8,9) have shown no evidence of rejection, nor have explanted homografts (6,10). Therefore, it has been concluded that cryopreservation causes the greatest delay and diminution of the expression of leukocyte adhesion molecules (11).…”

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confidence: 99%

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Histological and Immunohistological Study of Cryopreserved Aortic Valve Grafts: The Possibility of a Clinical Application for Cryopreserved Aortic Valve Xenograft

Yokose

Fukunaga²,

Tayama³

et al. 2002

Artificial Organs

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The influence of preservation techniques on xenogeneic immune responses was examined to investigate the possibility of their clinical use. Mongrel dogs and pigs were used as allogeneic and discordant xenogeneic donors of aortic valve grafts, respectively. The allografts and xenografts were implanted in the abdominal aorta of mongrel dogs after cryopreservation or after fresh preservation. Allografts and xenografts were explanted at Days 7 and 42 for histological and immunohistochemical examination. In the fresh preserved allografts and the fresh preserved xenografts, the media of aortic wall grafts were significantly acellular at 7 days. Significant aortic medial destruction and neointimal proliferation with significant destruction of the valve leaflets was observed at 42 days. However, no differences were found among the preservation techniques in the xenogeneic grafts. To alleviate the lack of homografts, approaches such as the tissue-engineered graft may become an alternative in patients who are acceptable candidates.

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Section: Discussionmentioning

confidence: 83%

mentioning

confidence: 99%

Histological and Immunohistological Study of Cryopreserved Aortic Valve Grafts: The Possibility of a Clinical Application for Cryopreserved Aortic Valve Xenograft

Yokose

Fukunaga²,

Tayama³

et al. 2002

Artificial Organs

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“…However, cells of recipient origin could be detected in the interior of these valves, as has been reported by us and others. 8,25 The results reported in this study suggest that HCVE may play an important role in the long-term function of allograft valve prostheses and that stimulation of a reendothelialization process in vivo or in vitro might be possible. This may limit degeneration due to mechanical stress.…”

Section: Discussionmentioning

confidence: 65%

Cardiac valve endothelial cells: Relevance in the long-term function of biologic valve prostheses

Simon

Wilhelmia

Steinhoff

et al. 1998

The Journal of Thoracic and Cardiovascular Surgery

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“…The same holds true for the use of DNA-profile analysis by PCR. 18 Chromosome banding techniques have also been used in this field of research. 5,19,20 This approach allows for an accurate sex determination, but it also requires tissue culture and therefore the outcome of studies using this technique should be interpreted with care.…”

Section: Discussionmentioning

confidence: 99%

Identification of host and donor cells in porcine homograft heart valve explants by fluorescencein situ hybridization

et al. 1997

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The pathogenesis of the primary tissue degeneration that limits the life‐span of aortic and pulmonary homografts has still not been revealed. Histopathological studies on homograft explants have not given definitive insight into the eventual fate of donor cells, nor have they demonstrated the assumed importance of host cell ingrowth into the graft tissue. In this experimental study, fluorescence in situ hybridization (FISH) is introduced as a new approach to examine the distribution of host and donor cells in homograft explants. Aortic valve replacement was performed with a cryopreserved porcine aortic homograft in three pigs; donor and recipient were of opposite sex. After 4 months, the grafts were explanted and examined by FISH using a biotinylated porcine Y‐chromosome‐specific library probe. Following probe detection with FITC‐conjugated avidin, a clear distinction could be made between cells of host and donor origin without distorting the histological integrity of the explants. There was ingrowth of donor cells into the graft aortic wall and into the valve leaflet, to some extent. In all explants, remaining donor cells were present, though decreased in number. The introduction of FISH in homograft heart valve research provides a powerful tool to study the fate of recipient and donor cellular elements in situ, and may therefore contribute to a better understanding of the histopathological processes that take place in transplanted homograft valves. © 1997 by John Wiley & Sons, Ltd.

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The allograft valve in heart transplantation and valve replacement: Genetic assessment of the origin of the cells by means of deoxyribonucleic acid profiles

Cited by 7 publications

References 10 publications

Histological and Immunohistological Study of Cryopreserved Aortic Valve Grafts: The Possibility of a Clinical Application for Cryopreserved Aortic Valve Xenograft

Histological and Immunohistological Study of Cryopreserved Aortic Valve Grafts: The Possibility of a Clinical Application for Cryopreserved Aortic Valve Xenograft

Cardiac valve endothelial cells: Relevance in the long-term function of biologic valve prostheses

Identification of host and donor cells in porcine homograft heart valve explants by fluorescencein situ hybridization

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