The alloherpesviral counterparts of interleukin 10 in European eel and common carp

Beurden, Steven J. van; Forlenza, Maria; Westphal, Adrie H.; Wiegertjes, G.F.; Haenen, O.L.M.; Engelsma, Marc

doi:10.1016/j.fsi.2011.08.004

Cited by 24 publications

(20 citation statements)

References 65 publications

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“…There are two potential N-glycosylation sites (N95 and N113), only one of which occurs in ebvIL-10 and cmvIL-10 (23, 61, 62). Although four conserved cysteine residues are present, ahvIL-10 contains no predicted N-glycosylation sites (57). Like cellular IL-10s and other vIL-10s, khvIL-10 and ahvIL-10 are each predicted to contain six ␣-helices (hA to hF) and appear to adopt a similar folded structure (57).…”

Section: Resultsmentioning

confidence: 99%

“…Among the Alloherpesviridae, genes with homology to IL-10 have been identified in both KHV and the eel herpesvirus Anguillid herpesvirus 1 (AngHV1), although none has yet been reported in the amphibian alloherpesviruses (3,56,57). In this study, we analyzed expression of the KHV IL-10 ortholog (khvIL-10) in koi fin (KF-1) cells and showed that it is highly expressed in infected carp tissue during the acute and reactivation phases of infection and at significantly lower levels during virus persistence at low temperature.…”

Section: K Oi Herpesvirus (Khv) Classified Taxonomically As the Typementioning

confidence: 99%

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Koi Herpesvirus Encodes and Expresses a Functional Interleukin-10

Sunarto

Liongue

McColl

et al. 2012

J Virol

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Section: Resultsmentioning

confidence: 99%

Section: K Oi Herpesvirus (Khv) Classified Taxonomically As the Typementioning

confidence: 99%

Koi Herpesvirus Encodes and Expresses a Functional Interleukin-10

Sunarto

Liongue

McColl

et al. 2012

J Virol

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“…Although the protein sequences of cyhv3Il10 and cIl10 share only limited (26.9%) sequence identity, sequence similarity is much higher (67.3%) and the predicted three-dimensional structure and position of amino acids crucially important for the presumed interaction with the Il10 receptor (Il10r) are highly conserved (36), suggesting a conservation of signaling by these two orthologous molecules, at least in carp. A previous study conducted in zebrafish embryos showed that downregulation of the il10r1 via a morpholino approach abrogated the response to both cyhv3Il10 and zebrafish Il10 (11).…”

Section: Discussionmentioning

confidence: 99%

Cyprinid Herpesvirus 3 Il10 Inhibits Inflammatory Activities of Carp Macrophages and Promotes Proliferation of Igm+ B Cells and Memory T Cells in a Manner Similar to Carp Il10

Piazzon

Wentzel

Tijhaar

et al. 2015

The Journal of Immunology

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Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of a lethal disease of carp and encodes for an Il10 homolog (ORF134). Our previous studies with a recombinant ORF134-deleted strain and the derived revertant strain suggested that cyprinid herpesvirus 3 Il10 (CyHV-3 Il10 [cyhv3Il10]) is not essential for viral replication in vitro, or virulence in vivo. In apparent contrast, cyhv3Il10 is one of the most abundant proteins of the CyHV-3 secretome and is structurally very similar to carp Il10 and also human IL10. To date, studies addressing the biological activity of cyhv3Il10 on cells of its natural host have not been performed. To address the apparent contradiction between the presence of a structurally conserved Il10 homolog in the genome of CyHV-3 and the lack of a clear phenotype in vivo using recombinant cyhv3Il10-deleted viruses, we used an in vitro approach to investigate in detail whether cyhv3Il10 exerts any biological activity on carp cells. In this study, we provide direct evidence that cyhv3Il10 is biologically active and, similarly to carp Il10, signals via a conserved Stat3 pathway modulating immune cells of its natural host, carp. In vitro, cyhv3Il10 deactivates phagocytes with a prominent effect on macrophages, while also promoting proliferation of Igm+ B cells and memory T cells. Collectively, this study demonstrates a clear biological activity of cyhv3Il10 on cells of its natural host and indicates that cyhv3Il10 is a true viral ortholog of carp Il10. Furthermore, to our knowledge, this is the first report on biological activities of a nonmammalian viral Il10 homolog.

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“…The Alloherpesviridae family is divided into the following four genera: Batrachovirus, Cyprinivirus, Ictalurivirus, and Salmonivirus [20]. The Cyprinivirus genus contains four species: three of them, cyprinid herpesvirus 1 (CyHV-1), cyprinid herpesvirus 2 (CyHV-2), and cyprinid herpesvirus 3 (CyHV-3), are associated with common carp or goldfish, while one of them, anguillid herpesvirus 1 (AngHV1), is associated with freshwater eels [127,128]. Cyprinid herpesviruses have been reported to cause serious mortality in common carp and crucian carp [129].…”

Section: Herpesvirus Genomesmentioning

confidence: 99%

Virus genomes and virus-host interactions in aquaculture animals

Zhang

Gui

2015

Sci. China Life Sci.

172

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Over the last 30 years, aquaculture has become the fastest growing form of agriculture production in the world, but its development has been hampered by a diverse range of pathogenic viruses. During the last decade, a large number of viruses from aquatic animals have been identified, and more than 100 viral genomes have been sequenced and genetically characterized. These advances are leading to better understanding about antiviral mechanisms and the types of interaction occurring between aquatic viruses and their hosts. Here, based on our research experience of more than 20 years, we review the wealth of genetic and genomic information from studies on a diverse range of aquatic viruses, including iridoviruses, herpesviruses, reoviruses, and rhabdoviruses, and outline some major advances in our understanding of virus-host interactions in animals used in aquaculture.

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The alloherpesviral counterparts of interleukin 10 in European eel and common carp

Cited by 24 publications

References 65 publications

Koi Herpesvirus Encodes and Expresses a Functional Interleukin-10

Koi Herpesvirus Encodes and Expresses a Functional Interleukin-10

Cyprinid Herpesvirus 3 Il10 Inhibits Inflammatory Activities of Carp Macrophages and Promotes Proliferation of Igm+ B Cells and Memory T Cells in a Manner Similar to Carp Il10

Virus genomes and virus-host interactions in aquaculture animals

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