The alteration of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) in the knee joints of osteoarthritis mice

Wang, Luling; Pi, Caixia; Sun, Jing; Cui, Yujia; Cai, Lu; Lan, Yuanchen; Gu, Jinning; Liu, Linfeng; Zhang, Geru; Guo, Lianyang; Zhang, Zhaowei; Guo, Qiang; Zheng, Liwei; Xie, Jing; Zhang, Demao

doi:10.1080/01478885.2020.1861908

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…It has been reported that a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs) and matrix metalloproteinases (MMPs) are critical to the degradation of the cartilage matrix in OA . Representative members of the ADAMTSs and MMPs family, including ADAMTS5, ADAMTS1, and MMP13, have been considered essential in OA cartilage erosion , and subchondral bone remodeling tissue. , Herein, RT-PCR of ADAMTS5, ADAMTS1, and MMP13 were subsequently performed to demonstrate changes in cartilage catabolism (Figure g). The stimulation of IL-1β activated the catabolic process of cartilage and induced the up-regulation of ADAMTS5, ADAMTS1, and MMP13 in chondrocytes.…”

Section: Resultsmentioning

confidence: 99%

Radical-Scavenging and Subchondral Bone-Regenerating Nanomedicine for Osteoarthritis Treatment

Wei

Liu

et al. 2023

ACS Nano

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Osteoarthritis (OA) is characterized by cartilage degradation and subchondral bone remodeling. However, most available studies focus on either cartilage degradation or subchondral bone lesion, alone, and rarely pay attention to the synergy of these two pathological changes. Herein, a dualfunctional medication is developed to simultaneously protect cartilage and achieve subchondral bone repair. Black phosphorus nanosheets (BPNSs), with a strong reactive oxygen species (ROS)scavenging capability and high biocompatibility, also present a notable promoting effect in osteogenesis. BPNSs efficiently eliminate the intracellular ROS and, thus, protect the inherent homeostasis between cartilage matrix anabolism and catabolism. RNA sequencing results of BPNSs-treated OA chondrocytes further reveal the restoration of chondrocyte function, activation of antioxidant enzymes, and regulation of inflammation. Additional in vivo assessments solidly confirm that BPNSs inhibit cartilage degradation and prevent OA progression. Meanwhile, histological evaluation and microcomputed tomography (micro-CT) scanning analysis verify the satisfying disease-modifying effects of BPNSs on OA. Additionally, the excellent biocompatibility of BPNSs enables them as a competitive candidate for OA treatment. This distinct disease-modifying treatment of OA on the basis of BPNSs provides an insight and paradigm on the dual-functional treatment strategy focusing on both cartilage degradation and subchondral bone lesion in OA and explores a broader biomedical application of BPNS nanomedicine in orthopedics.

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Section: Resultsmentioning

confidence: 99%

Radical-Scavenging and Subchondral Bone-Regenerating Nanomedicine for Osteoarthritis Treatment

Wei

Liu

et al. 2023

ACS Nano

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“…[ 94 ] Thus, peripheral complicated microenvironment factors and systemic hormone levels together determined the health and phenotype of chondrocytes. As an auxiliary, enhancement of metabolites such as creatinine, [ 95 ] overactivity of enzymes such as matrix metalloproteinase family (MMPs) targeted to collagen matrix, [ 96 ] enhanced hydrolysis of a disintegrin and metalloproteinase with thrombospondin motifs family (ADAMTS) targeted to aggrecan, [ 97 ] enhancement of xanthine oxidases to induced reactive oxygen species, [ 98 ] and excess of end products such as uric acid, [ 99 ] also cause damage to the cartilage and further complicate the patient's conditions.…”

Section: Discussionmentioning

confidence: 99%

Biomimetic Fibers Based on Equidistant Micropillar Arrays Determines Chondrocyte Fate via Mechanoadaptability

Zhou,

Yang,

Duan

et al. 2023

Adv Healthcare Materials

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It is recognized that the changes in the physical properties of extracellular matrix result in fine‐tuned cell responses including cell morphology, proliferation and differentiation. In this study, a novel patterned equidistant micropillar substrate based on polydimethylsiloxane (PDMS) was designed to mimic the collagen fiber‐like network of the cartilage matrix. By changing the component of the curing agent to an oligomeric base, we obtained micropillar substrates with the same topology but different stiffnesses and found that chondrocytes seeded onto the soft micropillar substrate maintain their phenotype by gathering type II collagen and aggrecan more effectively than those seeded onto the stiff micropillar substrate. Moreover, chondrocytes sensed and responded to micropillar substrates with different stiffnesses by altering the ECM‐cytoskeleton‐focal adhesion axis. We then found that the soft substrate‐preserved chondrocyte phenotype was dependent on the activation of Wnt/β‐catenin signaling at a high expression level. Finally, we indicated the changes in osteoid‐like region formation and cartilage phenotype loss in the stiffened sclerotic area of osteoarthritis mouse cartilage to validate the cell behavior changes triggered by micropillar substrates with different stiffnesses. This study provided the change in cell behaviors that are more similar to those of real chondrocytes at tissue level during the transition from a normal state to a state of osteoarthritis.This article is protected by copyright. All rights reserved

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Genome-wide identification of bovine ADAMTS gene family and analysis of its expression profile in the inflammatory process of mammary epithelial cells

Sheng

Zhang

Pan

et al. 2023

International Journal of Biological Macromolecules

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The alteration of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) in the knee joints of osteoarthritis mice

Cited by 4 publications

References 23 publications

Radical-Scavenging and Subchondral Bone-Regenerating Nanomedicine for Osteoarthritis Treatment

Radical-Scavenging and Subchondral Bone-Regenerating Nanomedicine for Osteoarthritis Treatment

Biomimetic Fibers Based on Equidistant Micropillar Arrays Determines Chondrocyte Fate via Mechanoadaptability

Genome-wide identification of bovine ADAMTS gene family and analysis of its expression profile in the inflammatory process of mammary epithelial cells

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