The ameliorating effect of silymarin against vancomycin-induced apoptosis and inflammation in rat liver

Güzel, Sevda; Şahinoğulları, Zuhal Uçkun; Canacankatan, Necmiye; Antmen, Şerife Efsun; Kibar, Deniz; Bayrak, Gülşen

doi:10.12991/jrp.2019.181

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…During the study, resveratrol was obtained from ProHealth in the USA (B094XH3W98), mixed with saline (0.9% NaCl) as suspension, and given to animals immediately, and vancomycin was obtained from Medis in Tunisia (AMM12/96860), dissolved in saline (0.9% NaCl). The dose of resveratrol was selected based on several studies that used 20 mg/kg to show its antioxidant and anti-inflammatory effects [ 38 , 39 , 40 ]; the vancomycin dose was selected based on its ability to produce toxic effects on several organs, including testicular tissues [ 41 , 42 , 43 ].…”

Section: Methodsmentioning

confidence: 99%

Resveratrol Ameliorates Vancomycin-Induced Testicular Dysfunction in Male Rats

Alshehri

2023

Medicina

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Background and Objectives: Numerous studies have indicated that antibiotics may adversely affect testicular and sperm function. As an alternative to penicillin, vancomycin is a glycopeptide antibiotic developed to treat resistant strains of Staphylococcus aureus. A few studies have suggested that vancomycin could cause testicular toxicity and apoptosis. Vancomycin, however, has not been investigated in terms of its mechanism of causing testicular toxicity. Materials and Methods: An experiment was conducted to investigate the effects of resveratrol (20 mg/kg, oral gavage) against vancomycin (200 mg/kg, i.p.) on the testicular function of Wistar rats for one week (7 days). There were three subgroups of animals. First, saline (i.p.) was administered to the control group. Then, in the second group, vancomycin was administered. Finally, vancomycin and resveratrol were administered in combination in the third group. Results: After seven days of vancomycin treatment, testosterone levels, sperm counts, and sperm motility were significantly reduced, but resveratrol attenuated the effects of vancomycin and restored the testosterone levels, sperm counts, and sperm motility to normal. In the presence of resveratrol, the vancomycin effects were attenuated, and the luteinizing hormone and follicular hormone levels were normalized after seven days of treatment with vancomycin. Histologically, vancomycin administration for seven days caused damage to testicular tissues and reduced the thickness of the basal lamina. However, the resveratrol administration with vancomycin prevented vancomycin’s toxic effects on testicular tissue. Conclusion: Resveratrol showed potential protective effects against vancomycin-induced testicular toxicity in Wistar rats.

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Section: Methodsmentioning

confidence: 99%

Resveratrol Ameliorates Vancomycin-Induced Testicular Dysfunction in Male Rats

Alshehri

2023

Medicina

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“…The experimental plan of the investigation is schematized in Figure 1 and illustrated in Table 1. The control and VCM groups were taken from our previous study (20). 7 VCM administration was applied at a dose of 400 mg/kg/day i.p.…”

Section: Experimental Designmentioning

confidence: 99%

“…A day after the initial administration of NAR, VCM administration (400 mg/kg/day) was initiated and proceeded along 7 days. a Control and VCM group were taken from our previous study (20); b VCM was administered 1 h after NAR administration; VCM: vancomycin; CMC: carboxymethyl cellulose; NAR: naringenin; VCM+NAR: VCM+NAR administered group.…”

Section: Experimental Designmentioning

confidence: 99%

Naringenin Reduces Hepatic Inflammation and Apoptosis Induced by Vancomycin in Rats

Şahinoğulları

Güzel

Canacankatan

et al. 2021

Clinical and Experimental Health Sciences

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Objective: This investigation aimed to detect the possible protective impacts of naringenin (NAR) on vancomycin (VCM)-induced liver toxicity through measuring caspase-3, -8 and -9 activities as markers of apoptosis and the levels of tumor necrosis factor-alpha, cyclooxygenase-2 and vascular endothelial growth factor as inflammation markers and assessing the histopathological alterations in rats. Methods:The rats were allocated into seven groups as, the control group (saline, intraperitoneally (i.p.)), VCM group (400 mg/kg/day, i.p.), carboxymethyl cellulose (CMC) group (0.5%, orally), NAR100 group (100 mg/kg/day, orally), VCM+NAR25 group (25 mg/kg/day, orally), VCM+NAR50 group (50 mg/kg/day, orally), VCM+NAR100 group (100 mg/kg/day, orally). The caspase enzyme activities and inflammation markers were measured using colorimetric methods and ELISA, respectively. Histopathological examinations were performed. Results:The caspase activities and levels of inflammation markers were significantly higher in the VCM group as opposed to the other groups. The caspase activities were significantly ameliorated in the VCM+NAR25 group compared to the VCM+NAR50 and VCM+NAR100 groups, but the levels of inflammation markers were significantly reduced in the VCM+NAR50 group and, especially, the VCM+NAR100 group compared to the VCM+NAR25 group. Conclusion:NAR has potential protective impact on liver injury caused by VCM, and the protective impacts of NAR at distinct doses may occur via different molecular mechanisms.

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