The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

Abstract

Context: The aqueous extracts of Cetraria islandica (L.) Ach. (Parmeliaceae) is traditionally used in many countries against a number of conditions, including inflammatory conditions. Objective: The present study aimed to assess, for the first time, the effectiveness of C. islandica in cultured primary blood cells of Type 1 diabetes subjects. Materials and methods: Diabetic and control blood samples were treated with or without aqueous lichen extract (5 and 10 mg mL À1) for 48 h. The activity of antioxidant enzymes in erythrocytes and also malondialdehyde levels in plasma were determined to evaluate the oxidative status. DNA damages were analyzed by SCE, MN and comet assays in cultured human lymphocytes. Additionally, proliferation index (PI) was evaluated in peripheral blood lymphocytes. Results: There were significant increases in observed total DNA damage (comet assay) (240.2%) and SCE (168.8%), but not in MN frequencies of cultures with diabetes as compared (p40.05) to controls. Whereas, the significant reductions of total DNA damage (69.2 and 65.3%) and SCE frequencies (17.7 and 12.3%) were determined when the 5 and 10 mg mL À1 lichen extract was added to the cell culture medium, respectively. However, lichen extract did not completely inhibit the induction of SCEs in lymphocytes of patients with diabetes. C. islandica extract was also useful on PI rates. Discussion: In conclusion, the antioxidant role of C. islandica in alleviating diabetes-induced genomic instability and for increasing cell viability was firstly indicated in the present study.

Biological activities of undescribed North American lichen species

“…Generally, antioxidant activity may be relevant as a mechanism for reversing cellular damage induced by metabolic dysfunction or by external factors. Radical scavenging capacity by several lichen extracts was reported recently, as well as in older work using extracts from cultured lichen material . Reports by Turkez and co‐workers suggest a protective role of lichen extracts against specific types of oxidative cell damage.…”

“…Radical scavenging capacity by several lichen extracts was reported recently, 3,4 as well as in older work using extracts from cultured lichen material. 5 Reports by Turkez and co-workers 6 suggest a protective role of lichen extracts against specific types of oxidative cell damage.…”

The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 Signalling

“…One of the previous studies was stated that C.islandica could be a scavenger of superoxide radical (Gulcin et al, 2002) based on the ingredient of lichen and linoleic acid. Lichen has also prevented mutagenesis and carcinogenesis in human (Colak et al, 2013).…”

“…So far, an enormous number of studies were conducted to identify novel, useful phototherapeutic agents including compounds derived from plants (Bontempo et al, 2015;Shweta, et al, 2013;Unnati Shah, 2013;Vaithiyanathan and Mirunalini, 2016), fungi (Shweta et al, 2013) and lichens (Kosanic, et al, 2013). It has suggested that lichens and their secondary metabolites can be used as an alternative method for the treatment of cancers (Kosanic et al, 2013(Kosanic et al, , 2014 due to its immune stimulation effect (Colak, et al, 2013). Recently, studies intended to show the effects of lichen extracts on various types of cancer are increasing (Coskun et al, 2015;Çelikler Kasımoğulları et al, 2014;He et al, 2010;Yang et al, 2014).…”

Efficiency of the Iceland moss extract in children with dry cough

“…В експерименті підтверджена антиоксидант на та антиканцерогенна дія цетрарії ісландської [19,22,24].…”