The Ameliorative Role of Acacia senegal Gum against the Oxidative Stress and Genotoxicity Induced by the Radiographic Contrast Medium (Ioxitalamate) in Albino Rats

Abstract

Arabic gum (Acacia senegal, AG) is proven effective antioxidant and cytoprotective agent. The present study was designed to test this notion by investigating the possible role of AG against the radiographic contrast medium (Ioxitalamate, Telebrix-35®, TBX)-induced oxidative stress and genotoxicity. Albino rats were divided into four groups and supplied with either; distilled water, daily 10% (w/v) AG, an intravenous dose of TBX (1600 mg I/kg b.wt) and co-administration of TBX and AG. Rats were sacrificed and blood samples were collected to assess the genotoxicity employing the peripheral blood leucocytes fluorescent double staining; namely the acridine orange/ethidium bromide (AO/EB) staining and alkaline comet assay. Further, chromosomal analyses were done in bone marrow cells. Serum urea and creatinine levels, in addition to malondialdehyde (MDA), nitric oxide (NO), catalase (CAT) and glutathione (GSH) levels in kidney tissues were measured. Liquid chromatography-mass spectrophotometry (LC-MS-MS) was performed to identify the chemical composition of AG extract. Kidney functions, single/double-stranded DNA damage, chromosomal aberrations, mitotic index, MDA and NO levels were significantly (p < 0.001) increased in TBX-treated group compared to the control and AG-treated one. Meanwhile, CAT and GSH activities were significantly diminished and the AG supplementation significantly (p < 0.001) ameliorated these effects compared with the control and AG-treated groups. Five compounds have been identified using GNPS networking including 7,3′,4′-Trihydroxyisoflavone, Noscapine, Tetrahydropapaveroline, Costunolide, Hesperidin. In conclusion, results of the present study suggest that AG exerted a protective role against TBX-induced oxidative stress and genotoxicity which may be attributed to the active metabolites in the gum.

Ameliorations in dyslipidemia and atherosclerotic plaque by the inhibition of HMG-CoA reductase and antioxidant potential of phytoconstituents of an aqueous seed extract of Acacia senegal (L.) Willd in rabbits

“…Several reports have provided the information about the ethnopharmacological applications of foods and herbal medicines of indigenous to the arid regions of African countries and the Indian subcontinent [ 16 – 18 ]. Acacia senegal (L.) Willd.is typically known by its common name, white gum tree, and is a member of the Leguminosae-Mimosoideae [ 11 , 19 ], while seed extracts of Acacia senegal (L.) Willd.is locally known as kumbat or kumatiya in Rajasthan [ 20 , 21 ]. The present study also identified the major phytoconstituents present in the seed extracts of Acacia senegal and assess its anti-atherosclerotic properties in hypercholesterolemic rabbits using a combination of in-vitro , in-silico , and in-vivo methodology.…”

Antibacterial, Antifungal, Cytotoxic and Genotoxic Activities of Different Extracts of Arabic and Myrrh Gums

“…Few studies have been carried out and showed that these gums were not genotoxic. 30 El-Garawani et al (2021) 31 suggested that Arabic gum exerted a protective role against TBX-induced oxidative stress and genotoxicity which may be attributed to its active metabolites. Primary scientific study done by Qureshi et al (1993), 32 aimed to assess cytotoxic and genotoxic effects of myrrha gum using normal and Ehrlich ascites carcinoma cell-bearing Swiss albino mice; results indicated that it had a significant cytotoxic potential and no genotoxic effect.…”

Optimization of Ultrasound-Assisted Extraction of Bioactive Compounds from Acacia Seyal Gum Using Response Surface Methodology and Their Chemical Content Identification by Raman, FTIR, and GC-TOFMS

“…However, the observed mass spectra consider the fingerprint of these compound that can be identified from the data library. It was widely reported that Arabic gum contained a considerable number of phytochemicals that exhibit antioxidant activity and can combat many diseases such as renal, cardiovascular, gastrointestinal, and respiratory diseases, due to its agonist effect on the oxidative stress and DNA damage [92]. Furthermore, Badreldin et al verified the antioxidative and anti-inflammatory properties of AG.…”