1993
DOI: 10.1111/j.1432-1033.1993.tb18208.x
|View full text |Cite
|
Sign up to set email alerts
|

The amino acid sequence of Ole e I, the major allergen from olive tree (Olea europaea) pollen

Abstract: The complete primary structure of the major allergen from Olea europaea (olive tree) pollen, Ole e I (IUIS nomenclature), has been determined. The amino acid sequence was established by automated Edman degradation of the reduced and alkylated molecule as well as of selected fragments obtained by proteolytic digestions. Ole e I contains a single polypeptide chain of 145 amino acid residues with a calculated molecular mass of 16331 Da. No free sulfhydryl groups have been detected in the native protein. The molec… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

8
188
0

Year Published

1994
1994
2021
2021

Publication Types

Select...
6
1

Relationship

3
4

Authors

Journals

citations
Cited by 137 publications
(196 citation statements)
references
References 50 publications
8
188
0
Order By: Relevance
“…Only one allergenic protein, Ole e 1, has been purified from olive-tree pollen, an important cause of pollinosis in the Mediterranean area. This allergen was characterized, cloned and expressed in Escherichia coli [4,26,281. Although several allergenic protein bands have been detected in olive pollen by immunoblotting assays [2,, the proteins have not been isolated.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Only one allergenic protein, Ole e 1, has been purified from olive-tree pollen, an important cause of pollinosis in the Mediterranean area. This allergen was characterized, cloned and expressed in Escherichia coli [4,26,281. Although several allergenic protein bands have been detected in olive pollen by immunoblotting assays [2,, the proteins have not been isolated.…”
Section: Discussionmentioning
confidence: 99%
“…Protein samples (1 -2 nmol) were hydrolyzed with 5.7 M HCI at 105°C for different lengths of time (24,48 and 96 h) as described [4]. Hydrolyzed samples were analyzed on a Beckman system 6300 amino acid analyzer.…”
Section: Methodsmentioning
confidence: 99%
“…The microheterogeneity observed in both olive [5] and lilac allergens is not exclusive to the Oleaceae family since it is also exhibited by a remarkable number of allergens from different natural sources. The most important pollen allergens, from a clinical point of view, show a polymorphic character; tree pollen allergens such as Cor a I [35], ragweed pollen allergens, such as Amb a I and Arnb a I1 [36] and grass allergens, such as Poa p IX and Lol p I [32, 371.…”
Section: E D V P Q P P I P Q F H I Q G Q V Y C D T C R a R F I T E Lmentioning
confidence: 99%
“…They obtained Ser and Tyr at positions 9 and 12, respectively, instead of Pro and His as in our case. Spectroscopic analysis (circular dichroism and fluorescence spectra; data not shown) of the isolated lilac protein indicates identical conformational characteristics to those of olive pollen allergen [5].…”
Section: Isolation and Characterization Of The Olive Allergen-like Prmentioning
confidence: 99%
See 1 more Smart Citation