The Amino Acid Transporter JhI-21 Coevolves with Glutamate Receptors, Impacts NMJ Physiology and Influences Locomotor Activity in Drosophila Larvae

Ziegler, Anna; Augustin, Hrvoje; Clark, Nathan L.; Berthelot-Grosjean, Martine; Simonnet, Mégane; Steinert, Joern R.; Geillon, Flore; Manière, Gérard; Featherstone, David E.; Grosjean, Yaël

doi:10.1038/srep19692

Cited by 23 publications

(22 citation statements)

References 62 publications

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“…BtAPC12 and BtAPC14 clustered with DmAPC7 (FBPP0088598), which helps regulate larval crawling behavior in Drosophila 57. For AAAP family genes, BtAAAP2 clustered with DmAAAP6 (CG3424), and BtAAAP11 clustered with ApAAAP7 (ACYPI001018).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide Identification and Expression Analysis of Amino Acid Transporters in the Whitefly, Bemisia tabaci (Gennadius)

et al. 2017

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The whitefly (Bemisia tabaci) is a cosmopolitan and devastating pest of agricultural crops and ornamentals. B. tabaci causes extensive damage by feeding on phloem and by transmitting plant viruses. Like many other organisms, insects depend on amino acid transporters (AATs) to transport amino acids into and out of its cells. We present a genome-wide and transcriptome-wide investigation of the following two families of AATs in B. tabaci biotype B: amino acid/auxin permease (AAAP) and amino acid/polyamine/organocation (APC). A total of 14 putative APCs and 25 putative AAAPs were identified, and a 10-paralog B. tabaci-specific expansion of AAAPs was found by maximum likelihood phylogeny. Detailed gene structure information revealed that 9 members of the B. tabaci-specific AAAP family expansion closely situated on a same scaffold. Expression profiling of the B. tabaci B APC and AAAP genes as affected by stage and plant host showed diverse expression patterns. The analysis of evolutionary rates indicated that purifying selection can explain the B. tabaci-specific AAAP expansion. RNA interference (RNAi)-mediated suppression of two AAAP genes (BtAAAP15 and BtAAAP21) significantly increased the mortality of B. tabaci B adults. The results provide a foundation for future functional analysis of APC and AAAP genes in B. tabaci.

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Section: Discussionmentioning

confidence: 99%

Genome-wide Identification and Expression Analysis of Amino Acid Transporters in the Whitefly, Bemisia tabaci (Gennadius)

et al. 2017

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“…Through computational methods such as ERC, we can exploit genetic divergence over evolutionary time to identify functional relationships between genes and pathways. Scanning for new genes exhibiting elevated ERC values with a specific pathway has led to the discovery of new genes affecting those functions (4,7,16). Because ERC has not yet been used to discover new DNA repair proteins, we demonstrate its potential through comparison to other screening methods.…”

Section: Significancementioning

confidence: 99%

Evolution-based screening enables genome-wide prioritization and discovery of DNA repair genes

Brunette

Jamalruddin

Baldock

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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DNA repair is critical for genome stability and is maintained through conserved pathways. Traditional genome-wide mammalian screens are both expensive and laborious. However, computational approaches circumvent these limitations and are a powerful tool to identify new DNA repair factors. By analyzing the evolutionary relationships between genes in the major DNA repair pathways, we uncovered functional relationships between individual genes and identified partners. Here we ranked 17,487 mammalian genes for coevolution with 6 distinct DNA repair pathways. Direct comparison to genetic screens for homologous recombination or Fanconi anemia factors indicates that our evolution-based screen is comparable, if not superior, to traditional screening approaches. Demonstrating the utility of our strategy, we identify a role for the DNA damage-induced apoptosis suppressor (DDIAS) gene in double-strand break repair based on its coevolution with homologous recombination. DDIAS knockdown results in DNA double-strand breaks, indicated by ATM kinase activation and 53BP1 foci induction. Additionally, DDIAS-depleted cells are deficient for homologous recombination. Our results reveal that evolutionary analysis is a powerful tool to uncover novel factors and functional relationships in DNA repair.

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“…JhI-21, a solute carrier 7-family amino acid transporter, is a novel marker found here in 5-HT neurons. This gene encodes for a protein necessary for protein nutrition signaling, which was recently described (42)(43)(44). However, these reports describe the importance of the JhI-21 protein peripherally, with no mechanism for the transmitting of nutritional information to the nervous system.…”

Section: Polyseq Defines Cell Typesmentioning

confidence: 99%

Comparative single-cell transcriptomics of complete insect nervous systems

Cocanougher

2019

Preprint

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SummaryMolecular profiles of neurons influence information processing, but bridging the gap between genes, circuits, and behavior has been very difficult. Furthermore, the behavioral state of an animal continuously changes across development and as a result of sensory experience. How behavioral state influences molecular cell state is poorly understood. Here we present a complete atlas of the Drosophila larval central nervous system composed of over 200,000 single cells across four developmental stages. We develop polyseq, a python package, to perform cell-type analyses. We use single-molecule RNA-FISH to validate our scRNAseq findings. To investigate how internal state affects cell state, we optogentically altered internal state with high-throughput behavior protocols designed to mimic wasp sting and over activation of the memory system. We found nervous system-wide and neuron-specific gene expression changes. This resource is valuable for developmental biology and neuroscience, and it advances our understanding of how genes, neurons, and circuits generate behavior.

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The Amino Acid Transporter JhI-21 Coevolves with Glutamate Receptors, Impacts NMJ Physiology and Influences Locomotor Activity in Drosophila Larvae

Cited by 23 publications

References 62 publications

Genome-wide Identification and Expression Analysis of Amino Acid Transporters in the Whitefly, Bemisia tabaci (Gennadius)

Genome-wide Identification and Expression Analysis of Amino Acid Transporters in the Whitefly, Bemisia tabaci (Gennadius)

Evolution-based screening enables genome-wide prioritization and discovery of DNA repair genes

Comparative single-cell transcriptomics of complete insect nervous systems

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