The amino acids of Autographa californica multiple nucleopolyhedrovirus P48 critical for the association with Ac93 are important for the nuclear egress of nucleocapsids and efficient formation of intranuclear microvesicles

Wang, Yan; He, Junjie; Mo, Mei Lan; Cai, Qingyun; Wu, Wenbi; Yuan, Meijin; Yang, Kai

doi:10.1016/j.virusres.2021.198644

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…Extensive research has shown that the formation of small NMVs isolated from INM requires at least four viral proteins including Ac75, Ac76, Ac93 and Ac103 [12,[18][19][20]; . These four proteins are also essential for the nuclear egress of nucleocapsids and ultimately regulate BV production.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to the NEC-mediated vesicle transport, baculovirus infection also induces the formation of NMVs to envelope virions in the nucleus [12]. As a well-studied large group of invertebrate-specific DNA viruses, baculoviruses possess a characteristic biphasic life cycle that produce two diverse phenotypic virions with identical genetic material, namely budded virion (BV) and occlusion-derived virion (ODV) [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of BmNPV Bm5 protein required for the formation of nuclear vesicle structures

Kong

Chen

et al. 2023

Journal of General Virology

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BmNPV infection induces nuclear vesicle-like structures and its Bm5 protein mediates the intranuclear lipid accumulation, which is thought to participate in the formation of nuclear vesicles. However, the relationship between viral-induced nuclear vesicles and Bm5 protein is still unclear. Here, our results indicated that BmNPV Bm5 protein participated in the baculovirus infection-induced nuclear vesicle-like structures' invagination thereby influencing the production of occlusion-derived virion (ODV) and occlusion body (OB). The process of nuclear vesicle-like structures' formation was dispensable for the transport or recruitment of ODV major envelope proteins, such as P74 and Bm14. Furthermore, baculovirus-induced nuclear F-actin might provide a direct mechanical force to mediate the scission of large vesicle-like structures from the nuclear membrane. Collectively, these findings illustrated a BmNPV Bm5 protein-induced nuclear membrane invagination pathway and revealed the function of nuclear vesicle-like structures in ODV production.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and characterization of BmNPV Bm5 protein required for the formation of nuclear vesicle structures

Kong

Chen

et al. 2023

Journal of General Virology

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CRISPR/Cas9-mediated disruption of orf76 as an antiviral therapy against BmNPV in the transgenic silkworm

Zhu,

Hu,

Chen

et al. 2024

International Journal of Biological Macromolecules

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Autographa californica multiple nucleopolyhedrovirus ac106 is required for the nuclear egress of nucleocapsids and intranuclear microvesicle formation

Mo,

Chen,

Yang

et al. 2024

J Virol

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Autographa californica multiple nucleopolyhedrovirus (AcMNPV) orf106 ( ac106 ) is highly conserved in baculoviruses. Previous studies have shown that ac106 is required for the production of infectious budded virions (BVs). However, the functional role of ac106 in virion morphogenesis remains unknown. In this report, an ac106 knockout virus and an ac106 repair virus were constructed. The effect of ac106 deletion on virion morphogenesis was investigated, and the expression and subcellular localization of the Ac106 protein were characterized. Our data indicated that ac106 is required for the nuclear egress of nucleocapsids and intranuclear microvesicle formation, as well as subsequent BV and occlusion-derived virion (ODV) production and the embedding of ODVs into polyhedra. Ac106 is a baculovirus late protein that is concentrated in discrete foci of virus-induced membrane structures in the intranuclear ring zone of virus-infected cells. Further studies on the relationship between Ac106 and four other proteins that are also required for intranuclear microvesicle formation, Ac75, Ac76, Ac93, and P48 (Ac103), revealed that Ac106 is associated with Ac75, Ac76, Ac93, P48, and itself. Ac106 is required for Ac75, Ac93, and P48 accumulation in foci of virus-induced intranuclear membrane structures and the intranuclear transport of Ac76. Analysis of the subcellular localization of ODV integral envelope proteins upon deletion of the genes required for intranuclear microvesicle formation indicated that intranuclear microvesicle formation may be essential for ODV integral envelope protein transport into the nucleus, supporting the hypothesis that intranuclear microvesicles originate from the nuclear membrane. IMPORTANCE Baculovirus occlusion-derived virions (ODVs) are known to acquire their envelopes from virus-induced intranuclear microvesicles within the nucleoplasm, and this strategy of intranuclear envelopment of nucleocapsids to form virions is unique among viruses. However, the mechanism of ODV morphogenesis, particularly intranuclear microvesicle formation, remains unclear. In this study, we identified ac106 as the fifth gene, in addition to ac75 , ac76 , ac93 , and p48 ( ac103 ), which are required for intranuclear microvesicle formation. Further studies on the relationship between ac106 and the other four genes, as well as the effect of ac106 or ac75 deletion on the localization of ODV integral envelope proteins, indicated that intranuclear microvesicle formation may be essential for the transport of ODV integral envelope proteins into the nucleus, which strongly supports the hypothesis that intranuclear microvesicles originate from the nuclear membrane. These findings greatly enhance our understanding of the molecular mechanism of baculovirus ODV morphogenesis.

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The amino acids of Autographa californica multiple nucleopolyhedrovirus P48 critical for the association with Ac93 are important for the nuclear egress of nucleocapsids and efficient formation of intranuclear microvesicles

Cited by 4 publications

References 39 publications

Identification and characterization of BmNPV Bm5 protein required for the formation of nuclear vesicle structures

Identification and characterization of BmNPV Bm5 protein required for the formation of nuclear vesicle structures

CRISPR/Cas9-mediated disruption of orf76 as an antiviral therapy against BmNPV in the transgenic silkworm

Autographa californica multiple nucleopolyhedrovirus ac106 is required for the nuclear egress of nucleocapsids and intranuclear microvesicle formation

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