The Amino Terminal Domain of HIV-1 Rev is Required for Discrimination of the RRE from Nonspecific RNA

Daly, Thomas J.; Doten, R C; Rusche, James R.; Auer, Manfred

doi:10.1006/jmbi.1995.0549

Cited by 34 publications

(29 citation statements)

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“…Figure 6A shows CD spectra of D-and L-RRE IIB in the absence of ligands. The D-RRE IIB spectrum is typical for A-form RNA (Daly et al 1995). As expected, the spectrum of L-RRE IIB appears as a mirror image of the D-RRE IIB spectrum.…”

Section: Differential CD Spectra Of L/d-rev and L/d-reversementioning

confidence: 62%

“…Binding occurred in the nanomolar range in all cases (Daly et al 1995). Whereas the interaction of complete Rev protein with RRE is relatively specific, a truncated Rev, lacking 20 aminoterminal residues, displays less ability to discriminate RRE from nonspecific RNA, yet still binds sense and anti-sense RNA species with high affinities (Daly et al 1995). It is reasonable to suggest that only when the peptides are highly structured within the intact protein, will they display a high degree of binding specificity for a particular RNA target.…”

Section: Discussionmentioning

confidence: 99%

“…For example, a 40-mer Rev peptide RP194, containing arginine-rich RRE-binding domain, failed to significantly discriminate with respect to binding affinities between native RRE, stem II, and a comparably sized antisense RRE construct, or even tRNA. Binding occurred in the nanomolar range in all cases (Daly et al 1995). Whereas the interaction of complete Rev protein with RRE is relatively specific, a truncated Rev, lacking 20 aminoterminal residues, displays less ability to discriminate RRE from nonspecific RNA, yet still binds sense and anti-sense RNA species with high affinities (Daly et al 1995).…”

Section: Discussionmentioning

confidence: 99%

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Stereospecificity of short Rev-derived peptide interactions with RRE IIB RNA

Litovchick¹,

Rando²

2003

RNA

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Section: Differential CD Spectra Of L/d-rev and L/d-reversementioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Stereospecificity of short Rev-derived peptide interactions with RRE IIB RNA

Litovchick¹,

Rando²

2003

RNA

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“…From the amino terminus of HIV-1 Rev, the ®rst 30 residues contain the NIS domain which is also required for discrimination of RRE from non-speci®c RNA binding (Daly et al, 1995). In the next 30 residues, the NOS domain is located, connected to the NIS by a proline-rich¯exible segment.…”

Section: Discussionmentioning

confidence: 99%

A cis-acting peptide signal in human immunodeficiency virus type I Rev which inhibits nuclear entry of small proteins

Kubota

Pomerantz

1998

Oncogene

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A peptide signal, which may control nucleo-cytoplasmic protein tracking, was newly identi®ed in human immunode®ciency virus type I (HIV-1) Rev, a lentiviral post-transcriptional transactivator. The sequence, in the amino-terminal portion of HIV-1 Rev, maintains a Rev mutant with a dysfunctional nuclear/nucleolar targeting signal outside of the nucleus, although this Rev molecule itself is small enough to pass through the nuclear pores. Transition of this sequence to the N-terminus of human T-lymphocytic leukemia/lymphoma virus type I (HTLV-I) p21 x , which is usually located evenly distributed throughout the cell, resulted in capture of p21x in the cytoplasm. Mutational analysis clari®ed that a 14 residue peptide sequence was sucient to display this inhibitory eect against nuclear entry. Furthermore, this HIV-1 Rev sequence was capable of inhibiting nuclear entry of a fragment of a human ribosomal protein, when it was fused to the carboxy terminus. The identi®ed nuclear entry inhibitory signal (NIS) contains a conserved hydrophilicity motif, which forms an amphipathic helix. Signi®cantly, this motif and its helical structure were shown to be important for NIS function and the HIV-1 Rev function itself. Possible roles for NIS as a molecular anchor are proposed herein.

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“…Based on these titration curves the dissociation constant for the ligand from Fe(III) in relation to the known dissociation constant for the competitor EDTA from Fe(III) was calculated. The K d for the reaction [3 ligand + Fe(III) ~ (ligand)3Fe(III)] determined by the algebraically correct description of the binding equilibria in a mixture of competing ligands [24] extended with the possibility for variable stochiometries between the ligands and substrate, is the intrinsic site dissociation constant for the case of the ligand binding to 3 equivalent non-interacting sites on Fe(Ill). The resulting K d values for the different chelators show that the estimation of equivalent extinction coefficients for 3 (ligand)3Fe(III) complexes provides an appropriate degree of sensitivity to structural variations between the ligands.…”

Section: Spectrophotometric Competition Titrationmentioning

confidence: 99%

Evaluation of the metal ion requirement of the human deoxyhypusine hydroxylase from HeLa cells using a novel enzyme assay

Csonga¹,

Ettmayer²,

Auer³

et al. 1996

FEBS Letters

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Hypusine synthesis in the eukaryotic initiation factor 5A is a unique two-step posttranslational modification. After deoxyhypusine is generated by the deoxyhypusine synthase, the deoxyhypusine hydroxylase (EC 1.14.99.29) catalyzes the formation of mature hypusine. A rapid assay for monitoring the deoxyhypusine hydroxylase activity was established, employing the oxidative cleavage of the hypusyl residue and subsequent extraction of the generated aldehydes. As metal ion chelators have been reported to inhibit the deoxyhypusine hydroxylase, the mechanism of this inhibition and the effect of transition metal ions on the enzyme activity were investigated. A ferric ion appears to be essential for enzymatic activity, the inhibition of which is entirely attributed to the metal ion binding capacity of the chelators.

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The Amino Terminal Domain of HIV-1 Rev is Required for Discrimination of the RRE from Nonspecific RNA

Cited by 34 publications

References 0 publications

Stereospecificity of short Rev-derived peptide interactions with RRE IIB RNA

Stereospecificity of short Rev-derived peptide interactions with RRE IIB RNA

A cis-acting peptide signal in human immunodeficiency virus type I Rev which inhibits nuclear entry of small proteins

Evaluation of the metal ion requirement of the human deoxyhypusine hydroxylase from HeLa cells using a novel enzyme assay

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