The amplifier role of T cells in the human in vitro B cell response to type 4 pneumococcal polysaccharide

Griffioen, Arjan W.; Toebes, Elly A.H.; Rijkers, Ger T.; Claas, Frans H.J.; Datema, Gert; Zegers, B. J. M.

doi:10.1016/0165-2478(92)90060-2

Cited by 25 publications

(13 citation statements)

References 23 publications

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“…3. As has been previously demonstrated, allogeneic CD4 + T lymphocytes were able to stimulate anti-caps-PS antibody production, albeit to a lesser extent than autologous CD4 + T lymphocytes [11]. CD4 + T lymphocytes obtained from a patient with the X-linked hyper-IgM syndrome completely failed to stimulate the immune response against caps-PS type 19F.…”

Section: Cd4 + T Lymphocytes Obtained From a Patient With Hyper-igm Smentioning

confidence: 59%

“…Up to now, only scarce evidence for a possible role of T lymphocytes and the CD40-CD40L interaction in the human immune response to caps-PS is available. Griffioen et al [11,20] provided evidence that CD4 + T lymphocytes stimulate and CD8 + T lymphocytes inhibit the human immune response to caps-PS in vitro. Leiva et al [12] showed induction of CD40L on T lymphocytes by stimulation with TI-2 antigens, which suggested that the CD40-CD40L interaction could play a role in the human immune response to caps-PS.…”

Section: Discussionmentioning

confidence: 99%

“…Already 10 years ago, Griffioen et al showed in in vitro studies that T lymphocytes are involved in the regulation of the human immune response to caps-PS [11]. Only more recently, Leiva et al showed that CD40L is upregulated in children immunized with caps-PS [12].…”

Section: Introductionmentioning

confidence: 99%

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The human antibody response to pneumococcal capsular polysaccharides is dependent on the CD40‐CD40 ligand interaction

et al. 2004

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Protection against infections with Streptococcus pneumoniae is mediated by antibodies against the capsular polysaccharides (caps-PS). Here we show that in in vitro experiments CD4 + T lymphocytes stimulate and CD8 + T lymphocytes inhibit the human anti-caps-PS antibody response. Using antagonistic anti-CD40 and antagonistic anti-CD40 ligand (CD40L) monoclonal antibodies, we showed that the CD4 + T lymphocyte-mediated stimulation is dependent on the CD40-CD40L interaction. The role of CD40L was further illustrated by the observation that CD4 + T lymphocytes obtained from a patient with hyper-IgM syndrome were unable to enhance the immune response to caps-PS. Furthermore, CD4 + T lymphocytes from cord blood, which did not express CD40L in response to stimulation with caps-PS, failed to stimulate the antibody response of adult B lymphocytes to caps-PS. These in vitro findings were confirmed by in vivo experiments in which SCID/SCID mice were reconstituted with human mononuclear cells. Furthermore, we showed that caps-PS induce production of IL-4, IL-6, IL-10, and IFN-+ , and that this enhanced production was inhibited by blocking the CD40-CD40L interaction. This is the first demonstration that the human immune response to caps-PS, which is markedly regulated by T lymphocytes, is dependent on the CD40-CD40L interaction.

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Section: Cd4 + T Lymphocytes Obtained From a Patient With Hyper-igm Smentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

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The human antibody response to pneumococcal capsular polysaccharides is dependent on the CD40‐CD40 ligand interaction

et al. 2004

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“…19 Although antipolysaccharide antibody responses are classically described as T-cell independent responses, there is increasing evidence to show that in humans at least, T cells are important in mounting a polysaccharide antibody response. 20 However, all patients had adequate classical protein T-cell dependent antibody responses and furthermore, all the non-SCID patients who have normal antipolysaccharide responses received chemotherapy. Thirdly, marrow TCD may play an important role in the subsequent immune development.…”

Section: Discussionmentioning

confidence: 99%

“…In all, 15 manipulated marrows were TCD in vitro with CAMPATH 1M, and two (patients 16, 21) received CD34+ enriched stem cells. Five patients received whole marrow infusions with no preparative chemotherapy (patients 8,9,10,19,20).…”

Section: Patient Populationmentioning

confidence: 99%

Polysaccharide antibody responses are impaired post bone marrow transplantation for severe combined immunodeficiency, but not other primary immunodeficiencies

Slatter

Bhattacharya

Flood

et al. 2003

Bone Marrow Transplant

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CD21 augments antigen presentation in immune individuals

1997

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CD21 (complement receptor 2, CR2) binds the C3 degradation products, iC3b and C3d, which are covalently linked to antigen or immune complexes in the process of complement activation. The ability of antigen-nonspecific B cells to present immune complexes containing high titers of acquired antibodies was tested. Influenza virus was incubated with serum from immune donors to create complement-containing complexes. These bound specifically to CD21 on transfected fibroblasts and B cell lines, as measured by microcytofluorimetry. Binding of immune complexes was ablated by inactivation of serum complement. In addition, the immunoglobulin in immune human serum blocked influenza binding to cells in the absence of complement, implying a minimal role for immunoglobulin-Fc receptor interactions in this system. Significant immune complex binding required a threshold level of CD21 expression, suggesting that only those cells with the highest levels of CD21 are likely to participate in the processing of macromolecular antigens. B cells pulsed with complement-influenza complexes elicited an augmented response from a panel of influenza-specific, class II-restricted T cell clones, as compared with those which had bound immunoglobulin-influenza complexes lacking complement. This enhanced response did not require CD35. In addition, B cell lines expressing higher levels of CD21 were more efficient in processing antigen than those with lower levels. These findings suggest that presentation of antigen by B cells in immune individuals is dependent on the binding of complement-antigen immune complexes to CD21.

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The amplifier role of T cells in the human in vitro B cell response to type 4 pneumococcal polysaccharide

Cited by 25 publications

References 23 publications

The human antibody response to pneumococcal capsular polysaccharides is dependent on the CD40‐CD40 ligand interaction

The human antibody response to pneumococcal capsular polysaccharides is dependent on the CD40‐CD40 ligand interaction

Polysaccharide antibody responses are impaired post bone marrow transplantation for severe combined immunodeficiency, but not other primary immunodeficiencies

CD21 augments antigen presentation in immune individuals

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